{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:41:41Z","timestamp":1773286901433,"version":"3.50.1"},"reference-count":47,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine matrix metalloproteinase\n(MMP) activity in synovial fluid (SF) obtained from the\njoints of dogs with degenerative joint disease (DJD)\nsecondary to various underlying conditions.<\/jats:p>\n\t\t\t\t<jats:p><jats:bold>Sample Population<\/jats:bold>\u201435 samples of SF obtained\nfrom 18 clinically normal (control) dogs and 34 samples\nof SF obtained from 17 dogs with DJD; dogs\nwith DJD were from 2 populations (client-owned\ndogs and research dogs that had DJD secondary to\nthe lysosomal storage disease mucopolysaccharidosis\nVII).<\/jats:p>\n\t\t\t\t<jats:p><jats:bold>Procedure<\/jats:bold>\u2014MMP activity in samples of SF was\nsemiquantitatively examined by use of gelatin or\ncasein zymography. Western blot analysis was performed\nby use of antibodies for MMP-2 and MMP-9.\nIn addition, in situ MMP activity was observed in sections\nof synovial membrane obtained from healthy\nand osteoarthritic joints.<\/jats:p>\n\t\t\t\t<jats:p><jats:bold>Results<\/jats:bold>\u2014Samples of SF from osteoarthritic joints\nhad higher MMP-2 activity and dramatically increased\nMMP-9 activity, compared with values for healthy\njoints. Substrate-overlay analyses indicated minimal\ngelatin-degrading activity in synoviocytes obtained\nfrom control dogs, whereas greater activity was seen\nin osteoarthritic synoviocytes, with additional activity\nin the underlying tissue.<\/jats:p>\n\t\t\t\t<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Higher\nMMP-2 activity and dramatic increases in MMP-9\nactivity were associated with the osteoarthritic\nstate, even though MMP-2 activity was detected\nin healthy joints. This study expands information\non MMP production in SF of osteoarthritic joints in\nother species and documents the similarity of\nMMP activity patterns regardless of the cause of\nDJD. 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