{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:34:08Z","timestamp":1767652448363,"version":"3.38.0"},"reference-count":31,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To perform molecular cloning of the\ncanine telomerase reverse transcriptase (TERT) gene\nand determine its expression in neoplastic and nonneoplastic\ncells.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u20149 canine tumor cell lines\nderived from various neoplasms, 16 primary canine\ntumors, and tissues from 15 normal canine organs.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Tumor cell lines were derived from\ncanine tumors that included osteosarcoma, mammary\ngland adenocarcinoma, melanoma, acute lymphoblastic\nleukemia, lymphoma, and mastocytoma\nand a canine primary fibroblast culture. Canine TERT\ncomplementary DNA (cDNA) was amplified by use of\npolymerase chain reaction (PCR) and sequenced.\nExpression of TERT mRNA was examined by reverse\ntranscription (RT)-PCR assay. Telomerase activity was\nmeasured by use of the telomeric repeat amplification\nprotocol assay.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014The canine TERT cDNA clone was 237 base\npairs in length and contained a central region encoding\nthe reverse transcriptase motif 2. Expression of\nTERT mRNA was detected in canine tumor cell lines\nthat had telomerase activity but not in telomerasenegative\ncanine primary fibroblasts. The TERT mRNA\nwas detected in 13 of 16 canine tumor tissues and\nseveral normal tissues such as liver, ovary, lymph\nnode, and thymus. A significant correlation between\nTERT expression level and telomerase activity was\nnoted.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Expression of\nTERT mRNA was closely associated with telomerase\nactivity in neoplastic cells as well as some non-neoplastic\ncells from dogs. In addition to telomerase activity,\nexpression of TERT mRNA can be used as a marker\nof tumor cells. 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