{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T21:10:08Z","timestamp":1775769008638,"version":"3.50.1"},"reference-count":22,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To determine the functionality of canine\nanti-\n                    <jats:italic>Malassezia<\/jats:italic>\n                    IgE via the passive transfer of immediate\nhypersensitivity localized to the skin (ie, cutaneous\nanaphylaxis) from atopic dogs with dermatitis\nattributable to overgrowth of\n                    <jats:italic>Malassezia pachydermatis<\/jats:italic>\n                    (\n                    <jats:italic>Malassezia<\/jats:italic>\n                    dermatitis [MD]) to healthy recipient\ndogs by use of the Prausnitz-K\u00fcstner (P-K) technique.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Animals<\/jats:bold>\n                    \u20147 clinically normal dogs, 32 atopic dogs\nwith MD, serum from 11 atopic dogs with MD, and 3\nhealthy dogs without prior sensitization to\n                    <jats:italic>M pachydermatis.<\/jats:italic>\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Serum from atopic dogs with MD was\nused for P-K tests in 3 clinically normal recipient\ndogs. Serial dilutions of untreated, heat-inactivated,\nIgE-absorbed, and bovine serum albumin (BSA)-\nabsorbed (control) aliquots of serum were injected\nID in triplicate for dermal sensitization. Twenty-four,\n48, and 72 hours later, a crude extract of\n                    <jats:italic>M pachydermatis<\/jats:italic>\n                    was injected ID into the sites used for\nsensitization injections, and immediate hypersensitivity\nreactions were graded on a 4-point scale.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014Untreated serum caused P-K reactivity\nbeginning 24 hours after passive sensitization and\npersisting through 72 hours (titers, 1:32 to 1:64).\nHeat inactivation and IgE-absorption of serum\neliminated P-K reactivity, whereas treatment of\nserum with BSA did not.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\n                    \u2014Analysis of\nP-K test results supports the passive transfer of cutaneous\nanaphylaxis by anti-\n                    <jats:italic>Malassezia<\/jats:italic>\n                    IgE and indicates\nit is functional in type-1 hypersensitivity reactions\nof atopic dogs with MD. Reduction or blockade\nof anti-\n                    <jats:italic>Malassezia<\/jats:italic>\n                    IgE in atopic dogs with MD may\nprovide better clinical control of the disease.\n(\n                    <jats:italic>Am J Vet Res<\/jats:italic>\n                    2003;64:262\u2013266)\n                  <\/jats:p>","DOI":"10.2460\/ajvr.2003.64.262","type":"journal-article","created":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T13:33:08Z","timestamp":1138023188000},"page":"262-266","source":"Crossref","is-referenced-by-count":23,"title":["Evaluation of serum obtained from atopic dogs with dermatitis attributable to Malassezia pachydermatis for passive transfer of immediate hypersensitivity to that organism"],"prefix":"10.2460","volume":"64","author":[{"given":"Daniel O.","family":"Morris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas 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