{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T10:38:03Z","timestamp":1780396683068,"version":"3.54.1"},"reference-count":31,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine the effect of immunization\nwith bovine luteinizing hormone receptor (LH-R) on\novarian function of cats.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u20149 adult female domestic cats.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u20147 cats were immunized with 0.5 mg of\nLH-R encapsulated in a silastic subdermal implant (3 \u00d7\n10 mm); 2 served as control cats. Receptors had 80%\nspecific binding to <jats:sup>125<\/jats:sup>I-human chorionic gonadotropin\nwith a binding capacity of 2,682 pM\/mg. Cats received\nbooster injections of LH-R. Cats were induced to ovulate\nwith luteinizing hormone (LH) releasing hormone\non day 345. Samples of venous blood and vaginal cells\nwere collected through day 395. Observation of estrus\nbehavior continued until day 516. Serum concentrations\nof estradiol, progesterone, thyroid gland hormones,\nLH, and LH-R antibody were determined.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014LH-R antibody was detected in the sera of\nimmunized cats within 21 days after implantation.\nDetection of LH-R antibody was associated with suppression\nof serum progesterone to \u2264 0.5 ng\/mL during\nthe study period, compared with concentrations of 5 to\n10 ng\/mL in control cats. Immunized cats did not display\nsigns of estrus. Release of LH after administration of LH releasing\nhormone indicated an intact hypothalamic-pituitary\naxis but poor corpus luteum function. Serum estradiol\nconcentrations remained between 30 to 40 pg\/mL\nin immunized and control cats. With the decrease antibody\ntiters, hormone concentrations returned to a pattern\nconsistent with that during fertility.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Active immunization\nwith LH-R suppressed corpus luteum function\nin cats. The effect was reversible. An LH-R-based\nantifertility vaccine may have clinical application in\nother vertebrates. 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