{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T23:07:34Z","timestamp":1785020854097,"version":"3.55.0"},"reference-count":22,"publisher":"American Veterinary Medical Association (AVMA)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To characterize the genetic diversity of\n<jats:italic>Haemophilus parasuis<\/jats:italic> field isolates with regard to\nserovar, herd of origin, and site of isolation.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample population<\/jats:bold>\u2014Isolates of <jats:italic>H parasuis<\/jats:italic> obtained\nfrom pigs in 15 North American herds and multi-farm\nsystems.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedur<\/jats:bold>e\u201498 <jats:italic>H parasuis<\/jats:italic> isolates were genotyped\nwith the enterobacterial repetitive intergeneic consensus\nbased-polymerase chain reaction (ERIC-PCR) technique\nand serotyped via agar gel precipitation test.\nGenomic fingerprints were analyzed and dendrograms\nwere constructed to identify strains from the same\nserovar group, herd of origin, or isolation site and to\nevaluate the genetic variability within these categories.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Serovar 4 (39%) and nontypeable (NT) isolates\n(27%) were most prevalent. Thirty-four distinct\nstrains were identified among the 98 isolates, using a\n90% similarity cutoff. Strains from serovar 4 and NT\nisolates had high genetic diversity (12 and 18 strains,\nrespectively). One to 3 major clusters of prevalent\nstrains could be identified in most of the evaluated\nherds. <jats:italic>Haemophilus parasuis<\/jats:italic> strains isolated from the\nupper respiratory tract were either serovar 3 or NT\nisolates. Potentially virulent strains (isolated from systemic\nsites) were either serovars 1, 2, 4, 5, 12, 13, or\n14, or NT isolates.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Although <jats:italic>H\nparasuis<\/jats:italic> had high genetic diversity overall, only a few\nstrains caused disease in these herds. The ERIC-PCR\ntechnique was more discriminative than serotyping,\nand a broad genetic variety was observed within particular\nserovar groups. (<jats:italic>Am J Vet Res<\/jats:italic> 2003;\n64:435\u2013442)<\/jats:p>","DOI":"10.2460\/ajvr.2003.64.435","type":"journal-article","created":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T12:21:06Z","timestamp":1135254066000},"page":"435-442","source":"Crossref","is-referenced-by-count":90,"title":["Characterization of the diversity of Haemophilus parasuis field isolates by use of serotyping and genotyping"],"prefix":"10.2460","volume":"64","author":[{"given":"Simone","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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