{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T21:10:22Z","timestamp":1775769022589,"version":"3.50.1"},"reference-count":50,"publisher":"American Veterinary Medical Association (AVMA)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To estimate receiver-operating characteristic\n(ROC) curves for a competitive ELISA (c-ELISA)\nthat is used in serodiagnosis of brucellosis in water\nbuffalo and cattle, to determine the most appropriate\npositive cutoff value for the c-ELISA in confirmation of\ninfection, and to evaluate species differences in\nc-ELISA function.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Sample population<\/jats:bold>\n                    \u2014Sera from 4 herds of cattle\n(n = 391) and 4 herds of water buffalo (381).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Serum samples were evaluated for\n                    <jats:italic>Brucella<\/jats:italic>\n                    -specific antibodies by use of a c-ELISA. On\nthe basis of previous serologic test results, iterative\nsimulation modeling was used to classify animals as\npositive or negative for\n                    <jats:italic>Brucella<\/jats:italic>\n                    infection without the\nuse of a gold standard. Accuracy of c-ELISA for diagnosis\nof infection was compared between cattle and\nwater buffalo by comparison of areas under ROC\ncurves.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014A positive cutoff value of 30% inhibition for\nc-ELISA yielded sensitivity and specificity estimates,\nrespectively, of 83.9 and 92.6% for cattle and 91.4 and\n95.4% for water buffalo. A positive cutoff value of\n35% inhibition yielded sensitivity and specificity estimates,\nrespectively, of 83.9 and 96.2% for cattle and\n88.0 and 97.4% for water buffalo. Areas under ROC\ncurves were 0.94 and 0.98 for cattle and water buffalo,\nrespectively.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion and Clinical Relevance<\/jats:bold>\n                    \u2014ROC curves\ncan be estimated by use of iterative simulation methods\nto determine optimal cutoff values for diagnostic\ntests with quantitative outcomes. A cutoff value of\n35% inhibition for the c-ELISA was found to be most\nappropriate for confirmation of\n                    <jats:italic>Brucella<\/jats:italic>\n                    infection in cattle\nand water buffalo. 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