{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:55:13Z","timestamp":1789026913642,"version":"build-2803163510"},"reference-count":19,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2003,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine whether previously unidentified\nvariations of the SzP protein of <jats:italic>Streptococcus\nequi <\/jats:italic>subsp <jats:italic>zooepidemicus<\/jats:italic> were present in horses\nwith various clinical signs of infection and whether\nany relationship could be identified between SzP protein\nvariants and naturally occurring clinical conditions.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u201423 isolates of <jats:italic>S equi<\/jats:italic> subsp\n<jats:italic>zooepidemicus<\/jats:italic> were recovered from specimens of\nhorses with various clinical conditions and used as a\nrepresentative population of isolates for evaluation of\ndifferent SzP protein variants.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Genetic heterogeneity of the isolates\nwas demonstrated by repetitive extragenic palindromic-\npolymerase chain reaction analysis. The <jats:italic>SzP<\/jats:italic>\ngene was sequenced and the presumed protein\nsequence determined for each isolate. Characteristics\nof the SzP proteins were compared among the isolates\nand in relation to the clinical conditions of horses\nfrom which they were recovered.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014The signal peptide types, number of proline-\nglutamic acid-proline-lysine repeats, and anchor\nsequences were consistent with those previously\ndescribed for the SzP protein. Many of the isolates\nclustered with 5 previously described types on the\nbasis of the hypervariable region of the SzP protein.\nOne additional variant, which represented 8 of the isolates,\nwas identified. Particular motifs in the hypervariable\nregion accounted for many of the differences\namong hypervariable types.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014The SzP protein\nappears to be limited to a selected number of\ntypes. Variations in the SzP protein are frequently\ndetermined on the basis of different motifs rather\nthan random amino acid substitutions. There does not\nappear to be any association of SzP protein variations\nand clinical manifestations of infection in horses.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2003;64:976\u2013981)<\/jats:p>","DOI":"10.2460\/ajvr.2003.64.976","type":"journal-article","created":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T13:31:21Z","timestamp":1135258281000},"page":"976-981","source":"Crossref","is-referenced-by-count":13,"title":["Identification of variations in SzP proteins of Streptococcus equi subspecies zooepidemicus and the relationship between protein variants and clinical signs of infection in horses"],"prefix":"10.2460","volume":"64","author":[{"given":"Richard L.","family":"Walker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Runyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"p_1_109","first-page":"31","volume":"6","author":"Sweeney CR","year":"1988","journal-title":"Am Coll Vet Intern Med"},{"key":"p_2_110","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.2460\/javma.1997.210.12.1774","volume":"210","author":"Carr EA","year":"1997","journal-title":"J Am Vet Med Assoc"},{"key":"p_3_111","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1093\/infdis\/155.6.1145","volume":"155","author":"Skjold SA","year":"1987","journal-title":"J Infect Dis"},{"key":"p_4_112","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2002.63.1298"},{"key":"p_5_113","doi-asserted-by":"crossref","first-page":"3600","DOI":"10.1128\/IAI.65.9.3600-3605.1997","volume":"65","author":"Timoney JF","year":"1997","journal-title":"Infect Immun"},{"key":"p_6_114","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1128\/IAI.63.4.1440-1445.1995","volume":"63","author":"Timoney JF","year":"1995","journal-title":"Infect Immun"},{"key":"p_7_115","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2000.61.162"},{"key":"p_8_116","doi-asserted-by":"crossref","first-page":"2772","DOI":"10.1128\/JCM.37.9.2772-2776.1999","volume":"37","author":"Alam S","year":"1999","journal-title":"J Clin Microbiol"},{"key":"p_9_117","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.2460\/ajvr.1998.59.09.1129","volume":"59","author":"Walker JA","year":"1998","journal-title":"Am J Vet Res"},{"key":"p_10_118","unstructured":"Swiss Institute of Bioinformatics. 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