{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:17:29Z","timestamp":1761707849364,"version":"3.38.0"},"reference-count":71,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2004,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To describe the distribution of mRNA that\ncodes for 8 subtypes of 5-hydroxytryptamine receptors\n(5-HTRs) in the digestive tract of dairy cows.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Fresh full-thickness wall specimens\nfrom the abomasum (fundus, corpus, and\nantrum), ileum, cecum, proximal loop of ascending\ncolon, and 4 locations of the spiral colon collected\nfrom 10 healthy cows at slaughter.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Concentrations of mRNA that code for\n5-HTR subtypes (5-HTR<jats:sub>1A<\/jats:sub>, 5-HTR<jats:sub>1B<\/jats:sub>, 5-HTR<jats:sub>1D<\/jats:sub>, 5-HTR<jats:sub>1F<\/jats:sub>,\n5-HTR<jats:sub>2A<\/jats:sub>, 5-HTR<jats:sub>2B<\/jats:sub>, 5-HTR<jats:sub>2C<\/jats:sub>, and 5-HTR<jats:sub>4<\/jats:sub>) in the bovine\ndigestive tract were measured by use of a quantitative\nreal-time reverse transcription-polymerase chain\nreaction assay. Results were reported in relation to\nmRNA expression of the housekeeping gene glyceraldehyde\nphosphate dehydrogenase (GAPDH).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Mean relative mRNA concentrations for 5-HTR were low (range, 0% to 1.32% of GAPDH), and\nmRNA that codes for 5-HTR<jats:sub>1A<\/jats:sub> was not detected. In\nthe abomasum, mRNA expression was highest for 5-HTR<jats:sub>1B<\/jats:sub> and 5-HTR<jats:sub>2B<\/jats:sub>, followed by subtypes 1F, 2A, 1D,\nand 4, whereas 5-HTR<jats:sub>2C<\/jats:sub> was not detected. In intestinal\nsamples, concentrations of subtypes 1B, 2B, and\n4 were highest, followed by 1D, 1F, 2A, and 2C.\nRelative concentrations of mRNA that code for 5-HTR<jats:sub>2A<\/jats:sub> were significantly higher in the abomasum\nthan the intestines, but lower for 5-HTR<jats:sub>2B<\/jats:sub>, 5-HTR<jats:sub>2C<\/jats:sub>,\nand 5-HT<jats:sub>R4<\/jats:sub>.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Relative concentrations\nof mRNA that code for 5-HTRs differ\namong locations in the gastrointestinal tract of cattle.\nUnderstanding differences in the distribution of 5-\nHTRs in healthy cattle and cattle with gastrointestinal\ntract disease may lead to improved therapeutic\napproaches for abomasal and cecal motility disorders.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2004;65:1151\u20131158)<\/jats:p>","DOI":"10.2460\/ajvr.2004.65.1151","type":"journal-article","created":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T09:00:20Z","timestamp":1135242020000},"page":"1151-1158","source":"Crossref","is-referenced-by-count":12,"title":["Distribution of mRNA that codes for 5-hydroxytryptamine receptor subtypes in the gastrointestinal tract of dairy cows"],"prefix":"10.2460","volume":"65","author":[{"given":"Mireille","family":"Meylan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teodora M.","family":"Georgieva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Reist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg 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