{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:43:43Z","timestamp":1762865023838,"version":"3.38.0"},"reference-count":22,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2004,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine the methicillin-resistant\nprofile of staphylococcal isolates from the skin of\ndogs with pyoderma.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201490 dogs with pyoderma.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Staphylococci isolated from dogs with\npyoderma were tested for susceptibility to methicillin\nby use of a standard disk diffusion test with oxacillin\ndisks. The DNA extracted from the isolates was tested\nfor the <jats:italic>mecA<\/jats:italic> gene that encodes the penicillinbinding\nprotein 2a (PBP2a) by use of a polymerase\nchain reaction (PCR) assay. The expression of PBP2a\nwas determined with a commercial latex agglutination\nassay. Species of staphylococcal isolates were\nidentified by use of morphologic, biochemical, and\nenzymatic tests.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Most of the isolated staphylococci were\nmethicillin-susceptible, coagulase-positive\n<jats:italic>Staphylococcus intermedius<\/jats:italic> isolates. Whereas only 2\nof 57 <jats:italic>S intermedius<\/jats:italic> isolates were resistant to methicillin,\napproximately half of the isolates had the <jats:italic>mecA<\/jats:italic>\ngene and produced PBP2a. S<jats:italic>taphylococcus schleiferi<\/jats:italic>\nwas the second most common isolate. Widespread\nresistance to methicillin was found among <jats:italic>S schleiferi<\/jats:italic>\nisolates. More coagulase-negative <jats:italic>S schleiferi<\/jats:italic> isolates\nwere identified with <jats:italic>mecA<\/jats:italic> gene-mediated resistance\nto methicillin, compared with coagulase-positive <jats:italic>S\nschleiferi<\/jats:italic> isolates.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014The latex\nagglutination assay for the detection of PBP2a\nexpression coupled with the PCR assay for the <jats:italic>mecA<\/jats:italic>\ngene may provide new information about emerging\nantimicrobial resistance among staphylococcal isolates.\n(<jats:italic>Am J Vet Res<\/jats:italic>2004;65:1265\u20131268)<\/jats:p>","DOI":"10.2460\/ajvr.2004.65.1265","type":"journal-article","created":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T09:43:47Z","timestamp":1135244627000},"page":"1265-1268","source":"Crossref","is-referenced-by-count":79,"title":["Methicillin resistance of staphylococci isolated from the skin of dogs with pyoderma"],"prefix":"10.2460","volume":"65","author":[{"given":"Stephen A.","family":"Kania","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola L.","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda A.","family":"Frank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca P.","family":"Wilkes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebekah D.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Bemis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"p_1_360","first-page":"274","author":"Scott DW","year":"2001","journal-title":"Philadelphia: WB Saunders Co"},{"key":"p_2_361","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00438-3"},{"key":"p_3_362","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2003.222.451"},{"key":"p_4_363","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1128\/JCM.31.4.812-818.1993","volume":"31","author":"Grattard F","year":"1993","journal-title":"J Clin Microbiol"},{"key":"p_5_364","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(89)90044-2"},{"key":"p_6_365","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1136\/vr.150.15.487","volume":"150","author":"Bes M","year":"2002","journal-title":"Vet Rec"},{"key":"p_7_366","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1099\/00207713-40-4-409","volume":"40","author":"Igimi S","year":"1990","journal-title":"Int J Syst Bacteriol"},{"key":"p_8_367","first-page":"2376","volume":"45","author":"Phillips","year":"1984","journal-title":"Am J Vet Res"},{"key":"p_9_368","doi-asserted-by":"crossref","first-page":"530","DOI":"10.2460\/javma.1993.203.04.0530","volume":"203","author":"Frank LA","year":"1993","journal-title":"J Am Vet Med Assoc"},{"key":"p_10_369","doi-asserted-by":"crossref","first-page":"2214","DOI":"10.1128\/JCM.36.8.2214-2219.1998","volume":"36","author":"Kluytmans J","year":"1998","journal-title":"J Clin Microbiol"},{"key":"p_11_370","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1093\/jac\/48.suppl_1.65","volume":"48","author":"Brown DF","year":"2001","journal-title":"J Antimicrob Chemother"},{"key":"p_12_371","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg325"},{"key":"p_13_372","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.18.5465-5472.2003"},{"key":"p_14_373","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.1128\/AAC.40.12.2680","volume":"40","author":"Kuwahara-Arai K","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"p_15_374","unstructured":"Jorgensen JH, Turnidge JD, Washington JA. 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