{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:35:23Z","timestamp":1792200923345,"version":"4.4.0"},"reference-count":60,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2004,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine the activity of recombinant\nfeline erythropoietin (rfEPO) in murine bioassays and\nevaluate its efficacy and safety in cats with erythropoietin-dependent nonregenerative anemia.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201426 cats (group 1, 19 cats with anemia\nattributed to chronic kidney disease [CKD]; group 2, 7\ncats with CKD and recombinant human erythropoietin\n[rhEPO]-induced red cell aplasia [RCA]).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014The rfEPO was synthesized by use of\nChinese hamster ovary (CHO) cells transfected with\nfeline erythropoietin complementary DNA. Preclinical\nassessments of rfEPO included an erythroid cell proliferation\nassay and measurements of reticulocytosis\nin Balb\/C mice. Clinical assessments of cats included\nhematologic, biochemical, and clinical examinations\nduring 12 (group 1) or 6 (group 2) months of rfEPO\ntreatment.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Biological activity of rfEPO was broadly\nequivalent to rhEPO in preclinical murine bioassays.\nMedian Hct and absolute reticulocyte count in cats\nincreased significantly during the first 3 weeks of\nrfEPO treatment, and median Hct generally could be\nmaintained within a target range of 30% to 40% with\nperiodic adjustments of rfEPO doses. Unexpectedly,\n5 cats in group 1 and 3 cats in group 2 that initially\nresponded to rfEPO treatment again developed anemia\nthat was refractory to additional rfEPO treatments,\neven at higher doses.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Treatment with\nrfEPO can reestablish active erythropoiesis in most\ncats with CKD, even those with anemia attributable to\nrhEPO-induced RCA. Unfortunately, development of\nRCA during treatment with CHO cell-derived recombinant\nerythropoietin proteins was not eliminated as a\nserious safety concern, even for this feline-specific\npreparation. 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