{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T08:58:01Z","timestamp":1789721881114,"version":"4.0.1"},"reference-count":45,"publisher":"American Veterinary Medical Association (AVMA)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To evaluate the diagnostic value of serum\nconcentrations of total magnesium (tMg) and ionized\nmagnesium (iMg), concentrations of magnesium\n(Mg) in muscle, intracellular Mg (icMg) concentrations,\nurinary Mg excretion (E<jats:sub>Mg<\/jats:sub>), Mg clearance (C<jats:sub>Mg<\/jats:sub>),\nand fractional clearance of Mg (FC<jats:sub>Mg<\/jats:sub>) in horses fed\ndiets with Mg content above and below National\nResearch Council recommendations.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u20149 young female horses.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedures<\/jats:bold>\u20146 horses were fed a reduced-Mg diet\nfor 29 days followed by an Mg-supplemented diet for\n24 days. Control horses (n = 3) were fed grass hay\nexclusively. Blood, urine, and tissue samples were\ncollected, and an Mg retention test was performed\nbefore and after restriction and supplementation of\nMg intake. Serum tMg, serum iMg, muscle Mg,\nicMg, and urine Mg concentrations were measured,\nand 24-hour E<jats:sub>Mg<\/jats:sub>, C<jats:sub>Mg<\/jats:sub>, and FC<jats:sub>Mg<\/jats:sub> were calculated.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Reductions in urinary 24-hour E<jats:sub>Mg<\/jats:sub>, C<jats:sub>Mg<\/jats:sub>, and\nFC<jats:sub>Mg<\/jats:sub> were evident after 13 days of feeding a reduced-Mg diet. Serum tMg and iMg concentrations, muscle\nMg content, and results of the Mg retention test\nwere not affected by feeding the Mg-deficient diet.\nSpot urine sample FC<jats:sub>Mg<\/jats:sub> accurately reflected FC<jats:sub>Mg<\/jats:sub> calculated\nfrom 6- and 24-hour pooled urine samples.\nMean \u00b1 SD FC<jats:sub>tMg<\/jats:sub> of horses eating grass hay was 29\n\u00b1 8%, whereas mean FC<jats:sub>tMg<\/jats:sub> for horses fed a reduced-Mg diet for 29 days was 6 \u00b1 3%.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014The 24-hour\nE<jats:sub>Mg<\/jats:sub> was the most sensitive indicator of reduced Mg\nintake in horses. Spot sample FC<jats:sub>Mg<\/jats:sub> can be conveniently\nused to identify horses consuming a diet deficient\nin Mg. 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