{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:04:54Z","timestamp":1791291894404,"version":"4.1.0"},"reference-count":29,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To develop an aerosol exposure method\nfor induction of brucellosis in rhesus macaques\n( <jats:italic>Macaca mulatta<\/jats:italic> ).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u201410 adult rhesus macaques.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u20148 rhesus macaques were challenge\nexposed with 10<jats:sup>2<\/jats:sup> to 10<jats:sup>5<\/jats:sup> colony-forming units of\n<jats:italic>Brucella melitensis<\/jats:italic> 16M by use of an aerosol-exposure\ntechnique, and 2 served as control animals. All\nmacaques were euthanatized 63 days after challenge\nexposure. Gross and microscopic lesions, bacterial\nburden in target organs, and histologic changes in tissues\nwere evaluated.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Grossly, spleen weights were increased in\nexposed macaques, compared with spleen weights in\ncontrol macaques. Histologically, there was inflammation\nin the liver, kidneys, spleen, testes, and epididymides\nin exposed macaques. The spleen and\nlymph nodes had increased numbers of lymphohistiocytic\ncells. Morphometrically, the spleen also had an\nincreased ratio of white pulp to red pulp. Areas of\nhepatitis and amount of splenic white pulp increased\nwith increasing exposure dose.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Pathologic\nfindings in rhesus macaques after aerosol exposure\nto B <jats:italic>melitensis<\/jats:italic> are similar to those observed in\nhumans with brucellosis.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Impact for Human Medicine<\/jats:bold>\u2014These results may aid\nin the development of a vaccine against brucellosis that\ncan be used in humans. 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