{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:51:29Z","timestamp":1775742689092,"version":"3.50.1"},"reference-count":44,"publisher":"American Veterinary Medical Association (AVMA)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2005,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine the immunogenicity of a\n<jats:italic>Moraxella bovis<\/jats:italic> cytolysin-enriched vaccine for prevention\nof infectious bovine keratoconjunctivitis (IBK).<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u2014104 mixed-breed beef calves ranging\nbetween 4 and 8 months of age.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Vaccines were prepared by the diafiltration\nof broth culture supernatant from hemolytic\n<jats:italic>M bovisor<\/jats:italic> or sterile media. The diafiltered retentate was\ncombined with Quil A adjuvant. Calves were randomly\nassigned to receive either the cytolysin vaccine\n(n = 35) or, as controls, adjuvant (35) or saline (0.9%\nNaCl) solution (34). Eyes of all calves were examined\nweekly for signs of IBK for 15 weeks. Calves that developed\nsevere IBK were treated SC with florfenicol.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Cytolysin vaccine contained 4 proteins with\nmolecular masses ranging between 65 and 90 kd.\nCytolysin-vaccinated calves had fewer instances of\nIBK than control calves. The time of onset of corneal\nlesions in cytolysin-vaccinated calves that developed\nIBK was delayed, compared with that of calves in\neither control group. The cytolysin-Quil A vaccine contained\nendotoxin, but calves did not have clinical signs\nof illness after vaccination.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Calves that\nwere vaccinated with a cytolysin-enriched vaccine had\nsome resistance to IBK. Vaccines containing concentrated\ndiafiltered <jats:italic>M bovis<\/jats:italic> cytolysin could protect beef\ncalves against IBK. 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