{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T13:37:20Z","timestamp":1788183440478,"version":"build-2803163510"},"reference-count":29,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2005,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine relative detection rates and\ndetection limits for 6 published polymerase chain\nreaction (PCR) assays used for detection of feline herpesvirus\ntype 1 (FHV-1) DNA.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u20145 vaccines licensed for use in\npreventing FHV-1\u2013associated disease; 15 conjunctival\nbiopsy specimens collected from cats with keratitis,\nconjunctivitis, or both; and a plaque-purified field isolate\nof FHV-1 cultured in vitro.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Vaccines and clinical samples were\nassessed for FHV-1 DNA by use of all 6 assays.\nDetection rates were calculated by assuming that any\nsample in which FHV-1 DNA was detected was a true-positive\nresult. Detection limits were estimated by\nuse of serial dilutions of DNA extracted from cultured\nFHV-1 and 1 clinical sample.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Testing by use of all 6 assays resulted in\ndetection of FHV-1 DNA in all 5 vaccines. Testing by\nuse of all 6 assays yielded concordant results for 9 of\n15 conjunctival biopsy specimens (8 with negative\nresults and 1 with a positive result). Calculated detection\nrates for clinical samples ranged from 29% to\n86%. Assay sensitivity was ranked similarly by use of\ndetection rate or detection limit.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Testing by\nuse of all assays was equally likely to detect vaccine\nvirus. Therefore, a positive PCR result in a cat may\nreflect vaccine virus rather than wild-type virus. Test\nsensitivity as assessed by detection limits and detection\nrates varied greatly. Because FHV-1 can be shed\nin clinically normal animals, high detection rate will\nnot necessarily correlate with high diagnostic sensitivity.\n(<jats:italic>Am J Vet Res<\/jats:italic> 2005;66:1550\u20131555)<\/jats:p>","DOI":"10.2460\/ajvr.2005.66.1550","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T12:25:59Z","timestamp":1135167959000},"page":"1550-1555","source":"Crossref","is-referenced-by-count":71,"title":["Relative sensitivity of polymerase chain reaction assays used for detection of feline herpesvirus type 1 DNA in clinical samples and commercial vaccines"],"prefix":"10.2460","volume":"66","author":[{"given":"David J.","family":"Maggs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather 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