{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:53:02Z","timestamp":1790729582356,"version":"4.1.0"},"reference-count":30,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2005,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To evaluate gross, histopathologic, and\nserum biochemical findings caused by <jats:italic>Leptospira\ninterrogans<\/jats:italic> serovars pomona and bratislava inoculated\nin dogs.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Animals<\/jats:bold>\u2014Twenty-seven 8-week-old female Beagles.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Dogs were randomly assigned to challenge\nor control groups. Challenge groups were conjunctivally\ninoculated on 3 successive days with\n5 \u00d710<jats:sup>7<\/jats:sup>\n\t\t\t\t\t<jats:italic>L interrogans<\/jats:italic> serovar pomona (n = 12) or\nserovar bratislava (11). Clinical signs were recorded\nthroughout the experiment, and clinical pathology\nassays, bacteriologic culture, and necropsies (6 or 7\ndogs necropsied at each time point) were done on\npostinoculation day (PID) 7, 10, 14, and 20.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014Infection could not be confirmed in any\nserovar bratislava\u2013inoculated dog, and control dogs\nremained healthy throughout the experiment.\nPositive culture and fluorescent antibody test results\nwere confirmed in 11 of 12 serovar pomona\u2013inoculated\ndogs. Fever and lethargy starting at PID 7 were\nthe most common clinical signs in serovar\npomona\u2013infected dogs. On day 10, gross lesions\nincluded multifocal renal and pulmonary hemorrhage\nand perirenal edema. Serovar pomona\u2013inoculated\ndogs had histopathologic lesions including hepatitis,\ninterstitial nephritis, and pneumonia at PID 7, 10, 14,\nand 20. Increases in BUN, anion gap, and bilirubin\nconcentration occurred on PID 10, 14, and 20.\nPlatelet counts in dogs with positive results of bacteriologic\nculture were decreased from baseline values\non PID 10, 12, and 14.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014\nConjunctival inoculation with L interrogans serovar\npomona resulted in a high rate of infection with concomitant\nhemorrhagic and inflammatory lesions of\nthe kidneys, liver, and lungs. 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