{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:10:03Z","timestamp":1791058203821,"version":"4.1.0"},"reference-count":36,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Objective<\/jats:bold>\u2014To determine whether cats in the northeastern\nUnited States develop serum antibodies\nagainst antigens of <jats:italic>Borrelia burgdorferi<\/jats:italic> and\n<jats:italic>Anaplasma phagocytophilum<\/jats:italic> and whether coinfection\nwith the 2 organisms occurs.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Sample Population<\/jats:bold>\u2014Serum samples from 84 healthy\ncats and 9 cats with lameness, fever, anorexia, or\nfatigue.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Procedure<\/jats:bold>\u2014Serum antibodies against <jats:italic>B burgdorferi<\/jats:italic>\nand <jats:italic>A phagocytophilum<\/jats:italic> were measured with an\nELISA incorporating a whole-cell preparation or purified\nrecombinant antigens, by means of western blot\nanalysis, or indirect fluorescent antibody (IFA) staining.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Results<\/jats:bold>\u2014ELISA results indicated that 44 of 93 (47%)\nsera contained antibodies against \u2265 3 <jats:italic>B burgdorferi<\/jats:italic>\nantigens, whereas 43 (46%) were reactive to wholecell\n<jats:italic>B burgdorferi<\/jats:italic>. Serum reactivity to protein 35, VlsE,\nand outer surface proteins A and F was most common.\nSeropositivity to \u2265 3 antigens occurred at the\nsame rate (5\/9) in the 9 ill cats as in the 84 healthy\ncats (46% [39\/84]). Of 13 sera reactive to recombinant\nantigens, 9 were seropositive as measured by\nwestern blot testing with whole-cell antigen.\nSeropositivity rates of 30% and 38% were detected\nfor antibodies against <jats:italic>A phagocytophilum<\/jats:italic> via IFA and\nELISA testing, respectively. Fifteen (16%) sera had\nantibodies against both pathogens.<\/jats:p>\n\t\t\t\t<jats:p>\n\t\t\t\t\t<jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Cats living in\nareas infested by <jats:italic>Ixodes scapularis<\/jats:italic> ticks are exposed\nto <jats:italic>B burgdorferi<\/jats:italic> and <jats:italic>A phagocytophilumand<\/jats:italic>, in some\ninstances, may be coinfected. Most cats appeared\nhealthy. An ELISA incorporating specific recombinant\nantigens may be used adjunctively with western blot\nand other assays to confirm <jats:italic>B burgdorferi<\/jats:italic> and\n<jats:italic>A phagocytophilum<\/jats:italic> infection in cats. (<jats:italic>Am J Vet Res<\/jats:italic>\n2005;66:1895\u20131899)<\/jats:p>","DOI":"10.2460\/ajvr.2005.66.1895","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T15:04:40Z","timestamp":1135177480000},"page":"1895-1899","source":"Crossref","is-referenced-by-count":40,"title":["Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats"],"prefix":"10.2460","volume":"66","author":[{"given":"Louis A.","family":"Magnarelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra L.","family":"Bushmich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob 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