{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T05:01:05Z","timestamp":1792645265990,"version":"4.5.0"},"reference-count":44,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2005,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Objective<\/jats:bold>\n                    \u2014To evaluate the efficacy of a novel monoclonal\nantibody (MAb)\u2013based dot-blot ELISA for\ndetection of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    antigens in urine samples of\ncattle.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Sample Population<\/jats:bold>\n                    \u2014Blood and urine samples of 45\ntest cattle from 5 farms in Chonburi province and 20\ncontrol cattle from 2 farms in Khon Kaen province in\nThailand.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Procedure<\/jats:bold>\n                    \u2014Blood and urine samples were assayed\n(microscopic agglutination test and urine antigen test)\nfor\n                    <jats:italic>Leptospira<\/jats:italic>\n                    infection by use of an MAb\u2013based dotblot\nELISA, and results for the ELISA were compared\nwith those for dark-field microscopy (DFM), microbial\nculture, and a polymerase chain reaction (PCR) assay.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    \u2014All urine samples with positive results for\nDFM, microbial culture, PCR assay, or &gt; 1 of these\ntests also had positive results when tested by use of\nthe MAb\u2013based dot-blot ELISA, except for 1 sample\nthat had positive results only for the PCR assay.\nDetection limits of the dot-blot ELISA were 10\n                    <jats:sup>3<\/jats:sup>\n                    leptospires\/\nmL of urine and 9.3 ng of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    homogenate. Comparison revealed that the diagnostic\nsensitivity, specificity, efficacy (accuracy), positive\npredictive value, and negative predictive value for the\nELISA were in agreement with results for DFM\n(100%, 72.72%, 80%, 57.14%, and 100%, respectively),\nmicrobial culture (100%, 61.54%, 66.62%,\n28.57%, and 100%, respectively), and PCR assay\n(95.45%, 100%, 91.77%, 100%, and 95.83%, respectively).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\n                    \u2014The\nMAb\u2013based dot-blot ELISA is suitable as a tool for\ndetecting leptospires in urine samples of cattle. (\n                    <jats:italic>Am\nJ Vet Res<\/jats:italic>\n                    2005;66:762\u2013766)\n                  <\/jats:p>","DOI":"10.2460\/ajvr.2005.66.762","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T10:16:44Z","timestamp":1135160204000},"page":"762-766","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of a monoclonal antibody\u2013based dot-blot ELISA for detection of Leptospira spp in bovine urine samples"],"prefix":"10.2460","volume":"66","author":[{"given":"Junpen","family":"Suwimonteerabutr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanpen","family":"Chaicumpa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patcharin","family":"Saengjaruk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pramuan","family":"Tapchaisri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manas","family":"Chongsa-nguan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thareerat","family":"Kalambaheti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pongrama","family":"Ramasoota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwaporn","family":"Sakolvaree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prachin","family":"Virakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"p_1_34","first-page":"955","volume":"43","author":"Prescott JF","year":"2002","journal-title":"Can Vet J"},{"key":"p_2_35","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/S1096-2867(96)80029-6","volume":"11","author":"Bolin CA","year":"1996","journal-title":"Semin Vet Med Surg (Small Anim)"},{"key":"p_3_36","unstructured":"Faine S, Adler B, Bolin C, etal Leptospira and leptospirosis. 2nd ed. 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