{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:57:18Z","timestamp":1793041038084,"version":"4.5.0"},"reference-count":52,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2022,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>OBJECTIVE<\/jats:title>\n                    <jats:p>\n                      To investigate the chondroprotective effects of autologous platelet-rich plasma (PRP), ampicillin-sulbactam (AmpS), or PRP combined with AmpS (PRP+AmpS) in an in vitro chondrocyte explant model of bovine\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      \u2013induced septic arthritis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>SAMPLE<\/jats:title>\n                    <jats:p>Autologous PRP and cartilage explants obtained from 6 healthy, adult, nonlactating Jersey-crossbred cows.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>ProcedureS<\/jats:title>\n                    <jats:p>\n                      Autologous PRP was prepared prior to euthanasia using an optimized double centrifugation protocol. Cartilage explants collected from grossly normal stifle joints were incubated in synovial fluid (SF) alone,\n                      <jats:italic>S aureus<\/jats:italic>\n                      \u2013inoculated SF (SA), or SA supplemented with PRP (25% culture medium volume), AmpS (2 mg\/mL), or both PRP (25% culture medium volume) and AmpS (2 mg\/mL; PRP+AmpS) for 24 hours. The metabolic activity, percentage of dead cells, and glycosaminoglycan content of cartilage explants were measured with a resazurin-based assay, live-dead cell staining, and dimethylmethylene blue assay, respectively. Treatment effects were assessed relative to the findings for cartilage explants incubated in SF alone.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Application of PRP, AmpS, and PRP+AmpS treatments significantly reduced\n                      <jats:italic>S aureus<\/jats:italic>\n                      \u2013induced chondrocyte death (ie, increased metabolic activity and cell viability staining) in cartilage explants, compared with untreated controls. There were no significant differences in chondrocyte death among explants treated with PRP, AmpS, or PRP+AmpS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CLINICAL RELEVANCE<\/jats:title>\n                    <jats:p>\n                      In this in vitro explant model of\n                      <jats:italic>S aureus<\/jats:italic>\n                      \u2013induced septic arthritis, PRP, AmpS, and PRP+AmpS treatments mitigated chondrocyte death. Additional work to confirm the efficacy of PRP with bacteria commonly associated with clinical septic arthritis in cattle as well as in vivo evaluation is warranted.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/ajvr.21.01.0007","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T11:49:06Z","timestamp":1638359346000},"page":"119-126","source":"Crossref","is-referenced-by-count":5,"title":["Autologous platelet-rich plasma effects on Staphylococcus aureus\u2013induced chondrocyte death in an in vitro bovine septic arthritis model"],"prefix":"10.2460","volume":"83","author":[{"given":"Andrew J.T.","family":"Muir","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Niehaus","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph W.","family":"Lozier","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara L.","family":"Cole","sequence":"additional","affiliation":[{"name":"Optical Microscopy Core, Notre Dame Integrated Imaging Facility, University of Notre Dame, Notre Dame, IN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zarah A.","family":"Belacic","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Ballash","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushmitha S.","family":"Durgam","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"8","key":"ref1","series-title":"Vet Rec.","first-page":"155","article-title":"Survey of lameness in British dairy cattle","volume":"111","author":"Russell AM","year":"1982","unstructured":"Russell AM, Rowlands GJ, Shaw SR, Weaver AD. 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