{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T05:10:04Z","timestamp":1749618604440,"version":"3.41.0"},"reference-count":58,"publisher":"American Veterinary Medical Association (AVMA)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec>\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>To investigate the time course of circulating neutrophil priming and activity in dogs with spinal cord injury secondary to intervertebral disk herniation that undergo decompressive surgery.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>ANIMALS<\/jats:title>\n<jats:p>9 dogs with spinal cord injury and 9 healthy dogs (controls).<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>PROCEDURES<\/jats:title>\n<jats:p>For dogs with spinal cord injury, blood samples were collected on the day of hospital admission and 3, 7, 30, and 90 days after injury and decompressive surgery. A single blood sample was collected from the control dogs. Flow cytometry analysis was performed on isolated neutrophils incubated with antibody against CD11b and nonfluorescent dihydrorhodamine 123, which was converted to fluorescent rhodamine 123 to measure oxidative burst activity.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>RESULTS<\/jats:title>\n<jats:p>Expression of CD11b was increased in dogs with spinal cord injury 3 days after injury and decompressive surgery, relative to day 7 expression. Neutrophils expressed high oxidative burst activity both 3 and 7 days after injury and decompressive surgery, compared with activity in healthy dogs.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>CLINICAL RELEVANCE<\/jats:title>\n<jats:p>For dogs with spinal cord injury, high CD11b expression 3 days after injury and decompressive surgery was consistent with findings for rodents with experimentally induced spinal cord injury. However, the high oxidative burst activity 3 and 7 days after injury and decompressive surgery was not consistent with data from other species, and additional studies on inflammatory events in dogs with naturally occurring spinal cord injury are needed.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.21.05.0073","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T19:16:24Z","timestamp":1642792584000},"page":"324-330","source":"Crossref","is-referenced-by-count":2,"title":["Circulating neutrophil activation in dogs with naturally occurring spinal cord injury secondary to intervertebral disk herniation"],"prefix":"10.2460","volume":"83","author":[{"given":"Rae L.","family":"Van Sandt","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"},{"name":"Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Jane","family":"Welsh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nick D.","family":"Jeffery","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin R.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dylan A.","family":"McCreedy","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gus A.","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"},{"name":"Flow Cytometry Facility, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Elizabeth","family":"Boudreau","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwendolyn J.","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan M.","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"issue":"5","key":"ref1","series-title":"Vet Surg","first-page":"884","article-title":"Extended durotomy to treat severe spinal cord injury after acute thoracolumbar disc herniation in dogs","volume":"49","author":"Jeffery ND","year":"2020","unstructured":"Jeffery ND, Mankin JM, Ito D,  Extended durotomy to treat severe spinal cord injury after acute thoracolumbar disc herniation in dogs. 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