{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:28:14Z","timestamp":1793042894264,"version":"4.5.0"},"reference-count":77,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2022,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p>Leptospirosis is an archetypal One Health problem as described in the companion Currents in One Health article in the October 2022 issue of the <jats:italic>Journal of the American Veterinary Medical Association<\/jats:italic> by Sykes et al. A thorough understanding of leptospirosis requires a detailed analysis of the elaborate interplay among pathogenic leptospiral strains, host species, and the environment. Such an understanding is required to inform appropriate preventative measures including vaccine design, prophylaxis efforts, educational programs that help to reduce exposure to pathogenic spirochetes, as well as policy development. Because of the complex epidemiology of leptospirosis, a One Health approach as defined by the One Health Initiative Task Force is critical\u2014an approach that calls for \u201cthe collaborative efforts of multiple disciplines working locally, nationally, and globally, to attain optimal health for people, animals and our environment.\u201d Over the last three decades, progressive advances in cutting-edge molecular typing techniques, as well as our ability to rapidly generate and share large amounts of sequence data through establishment and growth of databases, have been central to accelerating a One Health understanding of the epidemiology of leptospirosis. Nevertheless, our dependence on serotype information because of the serovar-specific nature of current vaccines means that laborious serotyping efforts continue. With the advent of new approaches such as mRNA vaccines that are based on lipopolysaccharide immunogens, sequence- and\/or proteomics-based typing methods may replace these methods.<\/jats:p>","DOI":"10.2460\/ajvr.22.06.0104","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T19:39:26Z","timestamp":1660851566000},"source":"Crossref","is-referenced-by-count":21,"title":["Understanding leptospirosis: application of state-of-the-art molecular typing tools with a One Health lens"],"prefix":"10.2460","volume":"83","author":[{"given":"Jane E.","family":"Sykes","sequence":"first","affiliation":[{"name":"Department of Medicine and Epidemiology, University of California-Davis, School of Veterinary Medicine, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandika D.","family":"Gamage","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Haake","sequence":"additional","affiliation":[{"name":"VA Greater Los Angeles Healthcare System, Los Angeles, CA"},{"name":"The David Geffen School of Medicine at UCLA, Los Angeles, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarlath E.","family":"Nally","sequence":"additional","affiliation":[{"name":"Infectious Bacterial Diseases Research Unit, USDA Agriculture Research Service, National Animal Disease Center, Ames, IA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"B1","series-title":"PLoS Negl Trop Dis","first-page":"e0003898","article-title":"Global morbidity and mortality of leptospirosis: a systematic review","volume":"9","year":"2015","unstructured":"Costa F, Hagan JE, Calcagno J, et al. 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Microb Genom. 2018;e000144."},{"key":"B70","series-title":"Front Microbiol","first-page":"816","article-title":"Biodiversity of environmental Leptospira: improving identification and revisiting the diagnosis","volume":"9","year":"2018","unstructured":"Thibeaux R, Girault D, Bierque E, et al. Biodiversity of environmental Leptospira: improving identification and revisiting the diagnosis. Front Microbiol. 2018;9:816. 29765361"},{"key":"B71","series-title":"Microbiol Immunol","first-page":"494","article-title":"A novel combination of selective agents for isolation of Leptospira species","volume":"55","year":"2011","unstructured":"Chakraborty A, Miyahara S, Villanueva SY, Saito M, Gloriani NG, Yoshida S. A novel combination of selective agents for isolation of Leptospira species. Microbiol Immunol. 2011;55:494\u2013501.21545510"},{"key":"B72","series-title":"Int J Environ Health Res","first-page":"107","article-title":"Detection of pathogenic Leptospira in ornamental water fountains from urban sites in Cali, Colombia","volume":"29","year":"2019","unstructured":"Escandon-Vargas K, Bustamante-Rengifo JA, Astudillo-Hernandez M. Detection of pathogenic Leptospira in ornamental water fountains from urban sites in Cali, Colombia. Int J Environ Health Res. 2019;29:107\u2013115.30213207"},{"key":"B73","series-title":"Water Res","first-page":"176","article-title":"Spatial and temporal dynamics of pathogenic Leptospira in surface waters from the urban slum environment","volume":"130","year":"2018","unstructured":"Casanovas-Massana A, Costa F, Riediger IN, et al. Spatial and temporal dynamics of pathogenic Leptospira in surface waters from the urban slum environment. 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