{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:46:57Z","timestamp":1777502817125,"version":"3.51.4"},"reference-count":28,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"sc_a1\">\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>To compare 2 point-of-care lateral flow assays (LFAs) with immunodiffusion (ID) IgG results for anti-coccidioidal antibody detection in dogs with coccidioidomycosis. A further aim was to compare the quantifiable output of 1 of the LFAs to ID antibody titers.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a2\">\n<jats:title>SAMPLE<\/jats:title>\n<jats:p>Serum banked from 73 client-owned dogs diagnosed with pulmonary or disseminated coccidioidomycosis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a3\">\n<jats:title>METHODS<\/jats:title>\n<jats:p>ID was used to determine antibody presence and titer against a coccidioidal antigen preparation. All sera were subsequently tested on an LFA based on recombinant chitinase 1 (CTS1) and the commercially available s\u014dna LFA. LFA results were analyzed and compared to ID IgG results and clinical diagnosis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a4\">\n<jats:title>RESULTS<\/jats:title>\n<jats:p>All assays showed similar sensitivities in detecting anti-coccidioidal antibodies (83.6% to 89.0%). When compared with ID IgG, the CTS1 LFA had a positive percent agreement of 100%, while the s\u014dna LFA had a positive percent agreement of 91.4%. Since the CTS1 LFA is semiquantitative, we were able to compare test line densities with ID titers and found a strong correlation between the 2 assays (Spearman \u03c1 = 0.82).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a5\">\n<jats:title>CLINICAL RELEVANCE<\/jats:title>\n<jats:p>This is the first side-by-side evaluation of a commercially available LFA (s\u014dna) and a newer more rapid anti-CTS1 antibody LFA using serum from dogs with coccidioidomycosis. Both LFAs tested have similar sensitivity to ID IgG results. The CTS1 LFA can be read after 10 minutes and is semiquantitative, while the s\u014dna LFA is read after 30 minutes, and the results are subject to interpretation. Accurate and fast detection of anti-coccidioidal antibodies allows clinicians to initiate appropriate treatment without diagnostic delay.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.23.12.0272","type":"journal-article","created":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T12:14:52Z","timestamp":1711455292000},"source":"Crossref","is-referenced-by-count":2,"title":["Two lateral flow assays for detection of anti-coccidioidal antibodies show similar performance to immunodiffusion in dogs with coccidioidomycosis"],"prefix":"10.2460","volume":"85","author":[{"given":"Francisca J.","family":"Grill","sequence":"first","affiliation":[{"name":"Cactus Bio, LLC, Phoenix, AZ"},{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic, Phoenix, AZ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared A.","family":"Jaffey","sequence":"additional","affiliation":[{"name":"Department of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Grys","sequence":"additional","affiliation":[{"name":"Cactus Bio, LLC, Phoenix, AZ"},{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic, Phoenix, AZ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura H.","family":"Rayhel","sequence":"additional","affiliation":[{"name":"Department of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, AZ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas F.","family":"Lake","sequence":"additional","affiliation":[{"name":"Cactus Bio, LLC, Phoenix, AZ"},{"name":"School of Life Sciences, Arizona State University, Tempe, AZ"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"issue":"1","key":"B1","series-title":"PLoS Med","first-page":"e2","article-title":"Coccidioidomycosis\u2014a fungal disease of the Americas","volume":"2","author":"Hector RF","year":"2005","unstructured":"Hector RF, Laniado-Laborin R. Coccidioidomycosis\u2014a fungal disease of the Americas. PLoS Med. 2005;2(1):e2."},{"issue":"3","key":"B2","series-title":"Clin Microbiol Rev","first-page":"505","article-title":"Recent advances in our understanding of the environmental, epidemiological, immunological, and clinical dimensions of coccidioidomycosis","volume":"26","author":"Nguyen C","year":"2013","unstructured":"Nguyen C, Barker BM, Hoover S, et al. Recent advances in our understanding of the environmental, epidemiological, immunological, and clinical dimensions of coccidioidomycosis. Clin Microbiol Rev. 2013;26(3):505\u2013525."},{"issue":"5","key":"B3","series-title":"Mycopathologia","first-page":"843","article-title":"Re-drawing the maps for endemic mycoses","volume":"185","author":"Ashraf N","year":"2020","unstructured":"Ashraf N, Kubat RC, Poplin V, et al. Re-drawing the maps for endemic mycoses. Mycopathologia. 2020;185(5):843\u2013865. doi:10.1007\/s11046-020-00431-2"},{"issue":"7","key":"B4","series-title":"J Fungi","first-page":"666","article-title":"Update on the epidemiology, diagnosis, and treatment of coccidioidomycosis","volume":"8","author":"Williams SL","year":"2022","unstructured":"Williams SL, Chiller T. Update on the epidemiology, diagnosis, and treatment of coccidioidomycosis. J Fungi. 2022;8(7):666."},{"issue":"11","key":"B5","series-title":"J Am Vet Med Assoc","first-page":"1846","article-title":"Incidence of Coccidioides infection among dogs residing in a region in which the organism is endemic","volume":"226","author":"Shubitz LE","year":"2005","unstructured":"Shubitz LE, Butkiewicz CD, Dial SM, Lindan CP. Incidence of Coccidioides infection among dogs residing in a region in which the organism is endemic. J Am Vet Med Assoc. 2005;226(11):1846\u20131850. doi:10.2460\/javma.2005.226.1846"},{"key":"B6","series-title":"Med Mycol","first-page":"S67","article-title":"Selected clinical features of coccidioidomycosis in dogs","volume":"57","author":"Davidson AP","year":"2019","unstructured":"Davidson AP, Shubitz LF, Alcott CJ, Sykes JE. Selected clinical features of coccidioidomycosis in dogs. Med Mycol. 2019;57(suppl 1):S67\u2013S75. doi:10.1093\/mmy\/myy113"},{"issue":"1","key":"B7","series-title":"J Am Vet Med Assoc","first-page":"79","article-title":"Cytologic analysis of tracheal wash specimens and bronchoalveolar lavage fluid in the diagnosis of mycotic infections in dogs","volume":"197","author":"Hawkins EC","year":"1990","unstructured":"Hawkins EC, DeNicola DB. Cytologic analysis of tracheal wash specimens and bronchoalveolar lavage fluid in the diagnosis of mycotic infections in dogs. J Am Vet Med Assoc. 1990;197(1):79\u201383. doi:10.2460\/javma.1990.197.01.79"},{"issue":"5","key":"B8","series-title":"J Am Anim Hosp Assoc","first-page":"226","article-title":"Coccidioidomycosis in dogs and cats: a review","volume":"44","author":"Graupmann-Kuzma A","year":"2008","unstructured":"Graupmann-Kuzma A, Valentine BA, Shubitz LF, Dial SM, Watrous B, Tornquist SJ. Coccidioidomycosis in dogs and cats: a review. J Am Anim Hosp Assoc. 2008;44(5):226\u2013235. doi:10.5326\/0440226"},{"key":"B9","series-title":"Top Companion Anim Med","first-page":"100754","article-title":"Diagnostic contribution of bronchoalveolar lavage sampling and fungal culture in a dog with pulmonary coccidioidomycosis","volume":"52","author":"Siler AD","year":"2023","unstructured":"Siler AD, Jaffey JA, Jacobs C, Shumway K. Diagnostic contribution of bronchoalveolar lavage sampling and fungal culture in a dog with pulmonary coccidioidomycosis. 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J Clin Microbiol. 2004;42(5):1982\u20131985. doi:10.1128\/JCM.42.5.1982-1985.2004"},{"issue":"1\u20132","key":"B12","series-title":"Mycopathologia","first-page":"151","article-title":"Coccidioidomycosis in biopsies with presumptive diagnosis of malignancy in dogs: report of three cases and comparative discussion of published reports","volume":"181","author":"Ram\u00edrez-Romero R","year":"2016","unstructured":"Ram\u00edrez-Romero R, Silva-P\u00e9rez RA, Lara-Arias J, et al. Coccidioidomycosis in biopsies with presumptive diagnosis of malignancy in dogs: report of three cases and comparative discussion of published reports. Mycopathologia. 2016;181(1\u20132):151\u2013157. doi:10.1007\/s11046-015-9948-4"},{"issue":"7","key":"B13","series-title":"Infect Immun","first-page":"2588","article-title":"The coccidioidal complement fixation and immunodiffusion-complement fixation antigen is a chitinase","volume":"60","author":"Johnson SM","year":"1992","unstructured":"Johnson SM, Pappagianis D. The coccidioidal complement fixation and immunodiffusion-complement fixation antigen is a chitinase. Infect Immun. 1992;60(7):2588\u20132592. doi:10.1128\/iai.60.7.2588-2592.1992"},{"issue":"6","key":"B14","series-title":"Infect Immun","first-page":"1992","article-title":"Molecular cloning and characterization of the Coccidioides immitis complement fixation\/chitinase antigen","volume":"64","author":"Yang C","year":"1996","unstructured":"Yang C, Zhu Y, Magee DM, Cox RA. Molecular cloning and characterization of the Coccidioides immitis complement fixation\/chitinase antigen. Infect Immun. 1996;64(6):1992\u20131997. doi:10.1128\/iai.64.6.1992-1997.1996"},{"issue":"12","key":"B15","series-title":"Infect Immun","first-page":"4967","article-title":"Cloning and expression of the complement fixation antigen-chitinase of Coccidioides immitis","volume":"64","author":"Zimmermann CR","year":"1996","unstructured":"Zimmermann CR, Johnson SM, Martens GW, White AG, Pappagianis D. 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J Vet Intern Med. 2021;35(5):2222\u20132231. doi:10.1111\/jvim.16243"},{"issue":"1","key":"B20","series-title":"J Vet Intern Med","first-page":"150","article-title":"Serum 25-hydroxyvitamin D concentrations in dogs with coccidioidomycosis and variables associated with extent of clinically evident disease","volume":"37","author":"Jaffey JA","year":"2023","unstructured":"Jaffey JA, Kreisler R, Shubitz LF, et al. Serum 25-hydroxyvitamin D concentrations in dogs with coccidioidomycosis and variables associated with extent of clinically evident disease. J Vet Intern Med. 2023;37(1):150\u2013160. doi:10.1111\/jvim.16629"},{"issue":"4","key":"B21","series-title":"PLoS One","first-page":"1","article-title":"Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species","volume":"12","author":"Chow NA","year":"2017","unstructured":"Chow NA, Lindsley MD, McCotter OZ, et al. 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Comparison of immunodiagnostic assays for the rapid diagnosis of coccidioidomycosis in dogs. J Fungi. 2022;8(7):728."},{"issue":"3","key":"B24","series-title":"Med Mycol","first-page":"328","article-title":"Evaluation of a commercially available, point-of-care Coccidioides antibody lateral flow assay to aid in rapid diagnosis of coccidioidomycosis in dogs","volume":"58","author":"Schlacks S","year":"2020","unstructured":"Schlacks S, Vishkautsan P, Butkiewicz C, Shubitz L. Evaluation of a commercially available, point-of-care Coccidioides antibody lateral flow assay to aid in rapid diagnosis of coccidioidomycosis in dogs. Med Mycol. 2020;58(3):328\u2013332. doi:10.1093\/mmy\/myz067"},{"issue":"2","key":"B25","series-title":"J Vet Intern Med","first-page":"1","article-title":"Clinical performance of a point-of-care Coccidioides antibody test in dogs","author":"Reagan KL","year":"2021","unstructured":"Reagan KL, McHardy I, Thompson GR 3rd, Sykes JE. 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