{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:57:16Z","timestamp":1793041036784,"version":"4.5.0"},"reference-count":34,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"sc_a1\">\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>To clarify the anti-inflammatory effect of platelet lysate (PL) on equine persistent synovitis by using a model of synovitis induced by monoiodoacetic acid (MIA).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a2\">\n<jats:title>METHODS<\/jats:title>\n<jats:p>Nonseptic synovitis was induced by administering MIA into both antebrachiocarpal joints of 6 clinically healthy horses on day 0. On days 23, 30, and 37, carpal circumference measurement and synovial fluid collection for assays (leucocytes, LDH, tumor necrosis factor-\u03b1, and TGF-\u03b21) were performed, after which PL was injected into 1 antebrachiocarpal joint and saline into the contralateral joint. Synovium and synovial fluid were obtained on day 44 for histological analysis and quantification of inflammation-related genes (<jats:italic>matrix metalloproteinase-13<\/jats:italic>, <jats:italic>a disintegrin and metalloproteinase with thrombospondin motifs 4<\/jats:italic>, <jats:italic>receptor activator of nuclear factor \u03ba-\u0392 ligand<\/jats:italic>, and <jats:italic>collagen type I \u03b12 chain<\/jats:italic>) and the abovementioned proteins.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a3\">\n<jats:title>RESULTS<\/jats:title>\n<jats:p>The LDH level on day 44 was significantly lower in the PL-injected joint than in the saline-treated one. However, no significant differences were found in the other indices quantified, including osteoclast counts on the synovium.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a4\">\n<jats:title>CONCLUSIONS<\/jats:title>\n<jats:p>Multiple IA administration of PL does not exert anti-inflammatory effects on the equine persistent synovitis induced by MIA.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a5\">\n<jats:title>CLINICAL RELEVANCE<\/jats:title>\n<jats:p>Intra-articular PL administration did not alter many inflammatory biomarkers, suggesting that PL does not have a direct anti-inflammatory effect. However, the reduction in synovial LDH levels suggests that PL promoted joint tissue repair and may consequently alleviate inflammation at the site of administration.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.24.03.0090","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T12:27:08Z","timestamp":1723811228000},"source":"Crossref","is-referenced-by-count":4,"title":["Platelet lysate does not have an anti-inflammatory effect on monoiodoacetic acid\u2013induced equine persistent synovitis"],"prefix":"10.2460","volume":"85","author":[{"given":"Kentaro","family":"Fukuda","sequence":"first","affiliation":[{"name":"Clinical Veterinary Medicine Division, Japan Racing Association, Equine Research Institute, Shimotsuke, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Mita","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine Division, Japan Racing Association, Equine Research Institute, Shimotsuke, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisuke","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine Division, Japan Racing Association, Equine Research Institute, Shimotsuke, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihisa","family":"Tamura","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine Division, Japan Racing Association, Equine Research Institute, Shimotsuke, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsutoshi","family":"Kuwano","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine Division, Japan Racing Association, Equine Research Institute, Shimotsuke, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumio","family":"Sato","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine Division, Japan Racing Association, Equine Research Institute, Shimotsuke, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Clinical Veterinary Medicine Division, Japan Racing Association, Equine Research Institute, Shimotsuke, Tochigi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"11","key":"B1","series-title":"Bone Joint Res","first-page":"297","article-title":"The horse as a model of naturally occurring osteoarthritis","volume":"1","author":"McIlwraith CW","year":"2012","unstructured":"McIlwraith CW, Frisbie DD, Kawcak CE. 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