{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T12:06:07Z","timestamp":1784030767227,"version":"3.55.0"},"reference-count":74,"publisher":"American Veterinary Medical Association (AVMA)","issue":"12","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2024,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"sc_a1\">\n<jats:title>OBJECTIVE<\/jats:title>\n<jats:p>The objective of this study was to investigate the differential expression of genes associated with coagulation in bovine monocyte-derived macrophages (MoM\u03a6) exposed to lipopolysaccharide (LPS) in vitro. We hypothesized that MoM\u03a6 stimulated with LPS would have upregulation of procoagulant genes and downregulation of genes protecting against coagulation.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a2\">\n<jats:title>METHODS<\/jats:title>\n<jats:p>MoM\u03a6 were isolated from Holstein steers and exposed to <jats:italic>Escherichia coli<\/jats:italic>\u2013derived LPS or a control for 3 hours. We used transcriptomics (RNA sequencing) to characterize the differential expression of genes associated with coagulation in the LPS-exposed MoM\u03a6 relative to the control group.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a3\">\n<jats:title>RESULTS<\/jats:title>\n<jats:p>1,602 genes were upregulated and 1,209 genes downregulated 3 hours after exposure to LPS. Monocyte-derived macrophages exposed to LPS displayed statistically significant upregulation of 4 proinflammatory genes, 2 anti-inflammatory genes, 8 genes involved in promoting coagulation, and 5 genes considered protective against coagulation. There was significant downregulation of 1 gene involved in the promotion of coagulation.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a4\">\n<jats:title>CONCLUSIONS<\/jats:title>\n<jats:p>Our results showed increased expression of most genes investigated promoting and protecting against coagulation and increased expression of proinflammatory and anti-inflammatory genes. These findings suggest that MoM\u03a6 exhibit a multifaceted response in the early response to LPS, promoting and protecting against excessive coagulation. This multifaceted response highlights the interplay between different pathways involved in early sepsis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a5\">\n<jats:title>CLINICAL RELEVANCE<\/jats:title>\n<jats:p>Our data demonstrate the utility of using MoM\u03a6 as a model system to investigate sepsis-associated coagulopathies. These insights into the early transcriptomic changes in response to LPS may help guide future research on the development of treatment modalities or diagnostic tests for patients with sepsis.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.24.04.0116","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T13:16:14Z","timestamp":1727702174000},"source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomic identification of genes associated with thrombosis and coagulation in lipopolysaccharide-exposed bovine monocyte-derived macrophages"],"prefix":"10.2460","volume":"85","author":[{"given":"Hannah R.","family":"Cohen","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle A.","family":"DeCourcey","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, University of Missouri, Columbia, MO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleverson","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"6917","key":"B1","series-title":"Nature","first-page":"885","article-title":"The immunopathogenesis of sepsis","volume":"420","author":"Cohen J","year":"2002","unstructured":"Cohen J. 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