{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T04:19:12Z","timestamp":1743567552926,"version":"3.40.3"},"reference-count":22,"publisher":"American Veterinary Medical Association (AVMA)","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2025,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:sec id=\"sc_a1\">\n<jats:title>Objective<\/jats:title>\n<jats:p>Pituitary pars intermedia dysfunction (PPID) is diagnosed by measuring the ACTH concentrations. Due to the reported instability of ACTH, it is recommended to transfer centrifuged plasma into cryovials; however, in practice, cryovials are infrequently used, and serum (red-top) tubes are used instead. This study investigated whether this procedure affects ACTH concentrations and the diagnosis of PPID.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a2\">\n<jats:title>Methods<\/jats:title>\n<jats:p>This was a cohort study. Blood was collected into EDTA tubes from 9 horses with PPID and 7 controls. After centrifugation, plasma was either aliquoted into a cryovial or into a serum tube. Samples were stored at 4 and 20 \u00b0C with ACTH concentrations measured using a chemiluminescent assay at 2, 24, and 48 hours after collection. Data were analyzed using a 2-way repeated measures ANOVA with <jats:italic>P<\/jats:italic> &lt; .05 considered significant.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a3\">\n<jats:title>Results<\/jats:title>\n<jats:p>There was a significant effect of time on ACTH concentrations in horses with PPID at 4 and 20 \u00b0C and in all horses at 20 \u00b0C with decreasing ACTH concentrations over time; however, no significant effect of storing plasma in a serum tube was detected. Three horses diagnosed with PPID would have had a different classification if the samples had been kept at 20 \u00b0C for 24 or 48 hours.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a4\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Time but not storage of plasma in serum tubes decreases the measured ACTH concentration in horses with PPID.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sc_a5\">\n<jats:title>Clinical Relevance<\/jats:title>\n<jats:p>Transferring plasma into serum tubes does not significantly alter the measured ACTH concentrations; however, time between collection and analysis might lead to erroneous diagnoses.<\/jats:p>\n<\/jats:sec>","DOI":"10.2460\/ajvr.24.11.0362","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T20:38:37Z","timestamp":1738874317000},"source":"Crossref","is-referenced-by-count":0,"title":["Time but not storage of equine plasma in silicate-coated tubes affects adrenocorticotropic hormone stability"],"prefix":"10.2460","volume":"86","author":[{"given":"Taylah J.","family":"Johnston","sequence":"first","affiliation":[{"name":"School of Veterinary Science, The University of Queensland, Gatton, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison J.","family":"Stewart","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, The University of Queensland, Gatton, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth L.","family":"Dryburgh","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health USA Inc, Duluth, GA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2820-8431","authenticated-orcid":true,"given":"Fran\u00e7ois-Ren\u00e9","family":"Bertin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Purdue University, West Lafayette, IN"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"issue":"2","key":"B1","series-title":"Vet Pathol","first-page":"390","article-title":"What's new in old horses? 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