{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T03:07:31Z","timestamp":1770174451714,"version":"3.49.0"},"reference-count":98,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"sc_a1\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To identify a panel of circulating microRNAs (miRNAs) that accurately discriminates dogs with splenic hemangiosarcoma from those with noncancerous splenic masses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a2\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serum samples from 10 dogs with hemangiosarcoma and 20 dogs with noncancerous splenic masses were included. Quantitative reverse transcription PCR was performed for 8 candidate miRNAs (miRNA [miR]-126-5p, miR-150-5p, miR-203a-3p, miR-214-3p, miR-452-5p, miR-494-3p, miR-497-5p, and miR-543) and 2 reference genes (miR-16-5p and miR-93-5p). Multivariable logistic regression was performed to identify a panel of miRNAs diagnostic for hemangiosarcoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a3\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The combination of miR-126-5p, miR-150-5p, miR-452-5p, and miR-543 had an area under the curve of 0.930, a sensitivity of 80%, a specificity of 90%, and a Youden\n                      <jats:italic>J<\/jats:italic>\n                      of 0.700 for discriminating dogs with hemangiosarcoma from those with noncancerous splenic masses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a4\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This panel of 4 miRNAs can be used to discriminate noncancerous splenic masses from hemangiosarcoma in dogs with splenic masses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a5\">\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>The ability to determine the nature of a splenic mass prior to surgery will provide veterinarians and their clients with important knowledge prior to surgery. If a mass is not hemangiosarcoma, owners may be more likely to proceed with surgery rather than euthanasia. Knowing that a mass is hemangiosarcoma will provide information to owners on the need for adjuvant therapy following surgery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/ajvr.25.07.0258","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T14:13:34Z","timestamp":1763388814000},"source":"Crossref","is-referenced-by-count":0,"title":["A panel of 4 circulating microRNAs (126-5p, 150-5p, 452-5p, and 543) discriminates dogs with splenic hemangiosarcoma from those with noncancerous splenic masses"],"prefix":"10.2460","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4554-8013","authenticated-orcid":true,"given":"Janet A.","family":"Grimes","sequence":"first","affiliation":[{"name":"Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer M.","family":"Schmiedt","sequence":"additional","affiliation":[{"name":"Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"issue":"4","key":"B1","series-title":"Vet Radiol Ultrasound","first-page":"289","article-title":"Comparison between malignant and nonmalignant splenic masses in dogs using contrast-enhanced computed tomography","volume":"45","author":"Fife WD","year":"2004","unstructured":"Fife WD, Samii VF, Drost WT, Mattoon JS, Hoshaw-Woodard S. 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