{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T03:16:47Z","timestamp":1770175007191,"version":"3.49.0"},"reference-count":130,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"sc_a1\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study evaluated the relationship between postthaw A-kinase anchoring protein 4 (AKAP4) fluorescence intensity and spermatological parameters in cryopreserved goat semen.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a2\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Semen was collected weekly by electroejaculation during the nonbreeding season, diluted, and cryopreserved using an automated freezing system. Postthaw sperm were assessed for total motility, progressive motility, and kinematics using computer-assisted semen analysis. Flow cytometry evaluated plasma membrane and acrosome integrity, high mitochondrial membrane potential, viability, and oxidative stress. The AKAP4 localization was determined by immunofluorescence, and intensity (X10\n                      <jats:sup>3<\/jats:sup>\n                      arbitrary units) was measured in 300 sperm\/sample. Cluster analysis examined categorical effects of AKAP4 intensity (low, medium, and high), and correlations assessed continuous effects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a3\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Ninety ejaculates from 18 goats (10 Boer and 8 Anglo-Nubian) were analyzed. A-kinase anchoring protein 4 localized to the acrosomal region. Low AKAP4 fluorescence intensity was linked to reduced total motility (45.3%; 95% CI, 41.1 to 49.5; partial \u03b7\n                      <jats:sup>2<\/jats:sup>\n                      = 0.128), progressive motility (14.3%; 95% CI, 12.5 to 16.1; partial \u03b7\n                      <jats:sup>2<\/jats:sup>\n                      = 0.131), and average path velocity, straight-line velocity, and beat cross frequency (partial \u03b7\n                      <jats:sup>2<\/jats:sup>\n                      = 0.105 to 0.114), while curvilinear velocity showed a moderate effect (partial \u03b7\n                      <jats:sup>2<\/jats:sup>\n                      = 0.060). The amplitude of lateral head displacement, straightness, linearity, wobble, plasma membrane, and acrosome integrity, viability, and reactive oxygen species were similar across groups (partial \u03b7\n                      <jats:sup>2<\/jats:sup>\n                      = 0.002 to 0.042). Mitochondrial membrane potential was lower in the low group (52.3%; 95% CI, 48.0 to 56.6; partial \u03b7\n                      <jats:sup>2<\/jats:sup>\n                      = 0.071) than in the medium group (62.2%; 95% CI, 58.3 to 66.1). No correlations were observed between AKAP4 and sperm parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a4\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A-kinase anchoring protein 4 intensity in frozen-thawed goat sperm affects energy metabolism and motility in a nonlinear, threshold-dependent manner, serving as a categorical sperm quality indicator.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a5\">\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>A-kinase anchoring protein 4 may serve as a biomarker for goat semen quality, particularly motility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/ajvr.25.10.0379","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T18:55:41Z","timestamp":1765997741000},"source":"Crossref","is-referenced-by-count":0,"title":["Postthaw A-kinase anchoring protein 4 fluorescence intensity is associated with sperm motility, key kinematic parameters, and mitochondrial activity in goat spermatozoa"],"prefix":"10.2460","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1326-2678","authenticated-orcid":true,"given":"Ahmet","family":"Eser","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Siirt University, Siirt, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3365-8960","authenticated-orcid":true,"given":"Asl\u0131han \u00c7ak\u0131r","family":"Cihangiro\u011flu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary 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T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2236-2526","authenticated-orcid":true,"given":"Ramazan","family":"Ar\u0131c\u0131","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Istanbul University-Cerrahpa\u015fa, Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9355-498X","authenticated-orcid":true,"given":"Selin","family":"Ya\u011fc\u0131o\u011flu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Istanbul University-Cerrahpa\u015fa, Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0786-6906","authenticated-orcid":true,"given":"\u0130kra","family":"Karaa\u011fa\u00e7","sequence":"additional","affiliation":[{"name":"Graduate Education Institute, Department of Reproduction and Artificial Insemination, Istanbul University-Cerrahpa\u015fa, Istanbul, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7354-0655","authenticated-orcid":true,"given":"\u00d6zg\u00fcr","family":"\u00d6z\u00f6ner","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Pathology, Siirt University, Siirt, T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5378-2726","authenticated-orcid":true,"given":"Merve Pekince","family":"\u00d6z\u00f6ner","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department 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Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview. Cell Tissue Bank. 2020;21(1):1\u201315. doi:10.1007\/s10561-019-09797-0"},{"issue":"1","key":"B2","series-title":"Biotechnol Anim Husb","first-page":"31","article-title":"Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams","volume":"38","author":"Ceki\u0107 B","year":"2022","unstructured":"Ceki\u0107 B, Kanzova H, Petrov G, Maksimovic N, \u0106osi\u0107 I, Milovanovi\u0107 A. Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams. 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Animals. 2024;14(18):2745. doi:10.3390\/ani14182745"},{"issue":"2","key":"B26","series-title":"J Urol","first-page":"414","article-title":"Proteomic signatures of sperm mitochondria in varicocele: clinical use as biomarkers of varicocele-associated infertility","volume":"200","author":"Samanta L","year":"2018","unstructured":"Samanta L, Agarwal A, Swain N, et al. Proteomic signatures of sperm mitochondria in varicocele: clinical use as biomarkers of varicocele-associated infertility. J Urol. 2018;200(2):414\u2013422. doi:10.1016\/j.juro.2018.03.009"},{"key":"B27","series-title":"Biochem Biophys Rep","first-page":"100916","article-title":"A simple method for quantitating confocal fluorescent images","volume":"25","author":"Shihan MH","year":"2021","unstructured":"Shihan MH, Novo SG, Le Marchand SJ, Wang Y, Duncan MK. A simple method for quantitating confocal fluorescent images. 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