{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:18:19Z","timestamp":1783009099437,"version":"3.54.5"},"reference-count":36,"publisher":"American Veterinary Medical Association (AVMA)","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n To evaluate the feasibility of biotin labeling of caprine RBCs and determine the posttransfusion survival of allogenic fresh and stored RBCs in healthy goats.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Fifteen 13- to 15-month-old nonlactating goats were randomly assigned to 3 groups (5\/group). Each goat served as a donor and recipient and was administered a whole-blood transfusion from a randomly assigned herd mate. The RBCs in whole blood were biotin labeled. Group 1 goats were transfused with fresh biotin-labeled whole blood within 6 hours of collection. Group 2 and 3 goats were transfused with biotin-labeled whole blood stored at 4\u2009\u00b0C for 14 and 28 days, respectively. Blood samples were collected at 15 minutes and 1, 2, 3, 5, 7, 14, 21, and 28 days after transfusion. Biotin-labeled RBCs were detected using flow cytometry, and their half-lives were determined using nonlinear regression. The study was conducted from June 10 through September 2, 2025.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Biotin-labeled RBCs were distinctly detected by flow cytometry. The half-lives of biotin-labeled RBCs in goats in groups 1, 2, and 3 were 4.6, 4.3, and 2.5 days, respectively. The half-lives of biotin-labeled RBCs in groups 1 and 2 were longer than in group 3 (Akaike information criterion difference, > 2). There was no difference in biotin-labeled RBC half-lives between groups 1 and 2 (Akaike information criterion difference, < 2).<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Biotin can be used to label caprine RBCs. The half-life of RBCs is affected by storage.<\/jats:p>\n <\/jats:sec>\n \n Clinical Relevance<\/jats:title>\n Blood storage for 28 days significantly reduces the half-life of biotin-labeled RBCs after transfusion.<\/jats:p>\n <\/jats:sec>","DOI":"10.2460\/ajvr.26.01.0019","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:02:39Z","timestamp":1773925359000},"source":"Crossref","is-referenced-by-count":0,"title":["Storage affects allogenic posttransfusion survival of red blood cells in healthy goats"],"prefix":"10.2460","volume":"87","author":[{"given":"Charissa Y.","family":"Tseng","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julyssa","family":"Castellanos","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meera C.","family":"Heller","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cileah M.","family":"Kretsch-York","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley S.","family":"Skebba","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anushka","family":"Panjwani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie W.","family":"Burges","sequence":"additional","affiliation":[{"name":"William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California-Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6841-2448","authenticated-orcid":true,"given":"Munashe","family":"Chigerwe","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, Davis, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"8","key":"B1","series-title":"Am J Vet Res","first-page":"960","article-title":"Use of an in vitro biotinylation technique for determination of posttransfusion survival of fresh and stored autologous red blood cells in Thoroughbreds","author":"Owens SD","year":"2010","unstructured":"Owens SD, Johns JL, Walker NJ, et al. Use of an in vitro biotinylation technique for determination of posttransfusion survival of fresh and stored autologous red blood cells in Thoroughbreds. Am J Vet Res. 2010;71(8):960\u2013966. doi:10.2460\/ajvr.71.8.960"},{"issue":"12","key":"B2","series-title":"J Am Vet Med Assoc","first-page":"1437","article-title":"Survival of erythrocytes after autologous and allogeneic transfusion in cats","author":"Marion RS","year":"1983","unstructured":"Marion RS, Smith JE. Survival of erythrocytes after autologous and allogeneic transfusion in cats. J Am Vet Med Assoc. 1983;183(12):1437\u20131439. doi:10.2460\/javma.1983.183.12.1437"},{"issue":"1","key":"B3","series-title":"Vet Clin Pathol","first-page":"56","article-title":"Post-transfusion survival of biotin-labeled allogeneic RBCs in adult horses","author":"Mudge MC","year":"2012","unstructured":"Mudge MC, Walker NJ, Borjesson DL, Librach F, Johns JL, Owens SD. Post-transfusion survival of biotin-labeled allogeneic RBCs in adult horses. Vet Clin Pathol. 2012;41(1):56\u201362. doi:10.1111\/j.1939-165X.2011.00384.x"},{"issue":"6","key":"B4","series-title":"J Vet Intern Med","first-page":"1683","article-title":"Survival time of cross-match incompatible red blood cells in adult horses","author":"Tomlinson JE","year":"2015","unstructured":"Tomlinson JE, Taberner E, Boston RC, Owens SD, Nolen-Walston RD. Survival time of cross-match incompatible red blood cells in adult horses. J Vet Intern Med. 2015;29(6):1683\u20131688. doi:10.1111\/jvim.13627"},{"issue":"3","key":"B5","series-title":"J Am Vet Med Assoc","first-page":"391","article-title":"Caprine whole blood appears sufficiently stable after 21 days in storage to be suitable for transfusion","author":"Neill HM","year":"2024","unstructured":"Neill HM, Scully CM, Liu CC, Baker RE. Caprine whole blood appears sufficiently stable after 21 days in storage to be suitable for transfusion. 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