{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:02:37Z","timestamp":1785513757921,"version":"3.56.0"},"reference-count":77,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,8,1]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n \n Campylobacter jejuni<\/jats:italic>\n is a leading zoonotic pathogen, with broiler chickens as a primary reservoir and source of human campylobacteriosis. This study investigated prevalence, phenotypic and genotypic antimicrobial resistance, and virulence genes in\n C jejuni<\/jats:italic>\n from broiler chicken samples at Egyptian slaughterhouses.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n 100 samples were randomly taken from slaughterhouses, comprising drippings (n = 50), neck skin (25), and cecal swabs (25).\n Campylobacter<\/jats:italic>\n isolates were identified by 23S rRNA and\n map<\/jats:italic>\n A gene.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n C jejuni<\/jats:italic>\n was isolated from 18 of 100 samples (18%). The\n C jejuni<\/jats:italic>\n isolates exhibited resistance against tetracycline (TE; 100%), amoxicillin (AMX; 83.3%), sulphamethoxazole-trimethoprim (SXT; 72.2%), and erythromycin (E; 66.7%). Meanwhile, the recovered isolates were found to be sensitive to meropenem (100%). There were 5 phenotypic resistance profiles: AMX, SXT, E, and TE (44.4%); AMX, E, and TE (22.2%); AMX, TE, and SXT (16.7%); TE and SXT (11.1%); and TE (5.6%).\n C jejuni<\/jats:italic>\n strains harbored the\n tet<\/jats:italic>\n (O) (100%) and\n bla<\/jats:italic>\n OXA-61<\/jats:sub>\n (83.3%) genes. There were 2 genotypic resistance profiles,\n bla<\/jats:italic>\n OXA-61<\/jats:sub>\n and\n tet<\/jats:italic>\n (O) (83.3%) and\n tet<\/jats:italic>\n O (16.7%).\n C jejuni<\/jats:italic>\n harbored\n cad<\/jats:italic>\n F,\n dna<\/jats:italic>\n J,\n fla<\/jats:italic>\n A, and\n cdt<\/jats:italic>\n B virulence genes with a prevalence of 100%, 100%, 44.4%, and 22.2%, respectively. There were 3 virulence profiles:\n cad<\/jats:italic>\n F\n +<\/jats:sup>\n ,\n dna<\/jats:italic>\n J\n +<\/jats:sup>\n ,\n fla<\/jats:italic>\n A\n +<\/jats:sup>\n , and\n cdt<\/jats:italic>\n B\n +<\/jats:sup>\n (22.2%);\n cad<\/jats:italic>\n F\n +<\/jats:sup>\n ,\n dna<\/jats:italic>\n J\n +<\/jats:sup>\n ,\n fla<\/jats:italic>\n A\n +<\/jats:sup>\n , and\n cdt<\/jats:italic>\n B\n \u2212<\/jats:sup>\n (27.8%); and\n cad<\/jats:italic>\n F\n +<\/jats:sup>\n ,\n dna<\/jats:italic>\n J\n +<\/jats:sup>\n ,\n fla<\/jats:italic>\n A\n \u2212<\/jats:sup>\n , and\n cdt<\/jats:italic>\n B\n \u2212<\/jats:sup>\n (50%).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our findings demonstrate that multidrug-resistant\n C jejuni<\/jats:italic>\n harboring key virulence\/resistance genes in broilers is a significant concern for both public health and poultry production.\n <\/jats:p>\n <\/jats:sec>\n \n Clinical Relevance<\/jats:title>\n \n The detection of multidrug-resistant, virulent\n C jejuni<\/jats:italic>\n underscores a substantial risk of carcass contamination and foodborne campylobacteriosis in humans.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.2460\/ajvr.26.02.0056","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T15:13:33Z","timestamp":1779117213000},"source":"Crossref","is-referenced-by-count":0,"title":["Molecular profiling of virulent, multidrug-resistant Campylobacter jejuni isolates from broiler flocks in Ismailia, Egypt"],"prefix":"10.2460","volume":"87","author":[{"given":"Reham M.","family":"El-Tarabili","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam S. E.","family":"Mustafa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Animal Health Research Institute, Ismailia, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Youssef","sequence":"additional","affiliation":[{"name":"Department of Pathology, Animal Health Research Institute, Ismailia, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherief M.","family":"Abdel-Raheem","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Veterinary Medicine, King Faisal University, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M. A.","family":"Meligy","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Central Lab, College of Veterinary Medicine, King Faisal University, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Enany","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"2","key":"B1","series-title":"J World Poult Res","first-page":"168","article-title":"Campylobacteriosis in poultry: a review","author":"Sadek SA","year":"2023","unstructured":"Sadek SA, Shaapan RM, Barakat AM. Campylobacteriosis in poultry: a review. 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