{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:05:17Z","timestamp":1790874317164,"version":"4.1.0"},"reference-count":24,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"sc_a1\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To evaluate the onset of immunity 7 days after administration of a live-attenuated\n                      <jats:italic>Bordetella bronchiseptica<\/jats:italic>\n                      (Bb) vaccine (Vanguard B Oral) in dogs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a2\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In a randomized, blinded, controlled study conducted at an animal research facility in 2022, forty 8-week-old Beagle puppies were randomly assigned to 2 groups (n = 20\/group) and administered a single oral dose of either placebo or vaccine on day 0. On day 7 after vaccination, all dogs were challenged with aerosolized virulent Bb. Clinical observations were conducted twice on days 0 and 6 through 20 and once daily on days 1 through 5 and 21. Tympanic temperatures were measured twice on day 0 and once daily from days 1 through 21. Microplate agglutination titers were collected from all animals on days \u221212, 0, 6, and 21.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a3\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Coughing on 2 consecutive days occurred in 17 of 20 control dogs (85%) versus 4 of 20 vaccinates (20%). Vaccinated dogs also exhibited fewer coughing observation periods (3.2% vs 15.8%) and a shorter coughing duration of 2.4 days (95% CI, 1.0 to 3.7) than controls (5.5 days; 95% CI, 4.1 to 6.8). Total clinical scores were significantly lower in vaccinated (median, 15.0) dogs than in controls (median, 27.0) and remained significant when ocular and nasal discharge were excluded.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a4\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The vaccine was considered safe, with only sporadic mild clinical signs. After challenge, vaccinated dogs demonstrated a significant reduction in coughing and other clinical signs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a5\">\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>Vanguard B Oral elicited a rapid onset of protective immunity against virulent Bb challenge as early as 7 days after vaccination.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/ajvr.26.03.0100","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:42:00Z","timestamp":1782837720000},"source":"Crossref","is-referenced-by-count":0,"title":["Oral administration of a live-attenuated Bordetella bronchiseptica vaccine (Vanguard B Oral) induces protective immunity against challenge 7 days after vaccination in dogs"],"prefix":"10.2460","volume":"87","author":[{"given":"Sharon","family":"Wappel","sequence":"first","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Inc, Kalamazoo, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sridhar","family":"Velineni","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Inc, Kalamazoo, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vickie","family":"King","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Inc, Kalamazoo, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eline","family":"Abma","sequence":"additional","affiliation":[{"name":"VMRD Regulatory Affairs, Zoetis Inc, Zaventem, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacquelien","family":"Hoevers","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Inc, Kalamazoo, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Martorell","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Manufacturing and Research, Girona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheyenne","family":"Houser","sequence":"additional","affiliation":[{"name":"VMRD Animal Research Support, Zoetis Inc, Richland, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Tague","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Inc, Kalamazoo, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Wilmes","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Inc, Lincoln, NE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Millership","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Research and Development, Zoetis Inc, Kalamazoo, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"2","key":"B1","series-title":"Am J Vet Res","first-page":"266","article-title":"Canine parainfluenza\u2013Bordetella bronchiseptica vaccine immunogenicity","volume":"42","author":"Chladek DW","year":"1981","unstructured":"Chladek DW, Williams JM, Gerber DL, Harris LL, Murdock FM. 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