{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:14:57Z","timestamp":1788268497997,"version":"build-2803163510"},"reference-count":39,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The objective of this study was to compare 4 different fecal diagnostic methods for detection and recovery of\n                    <jats:italic>Echinococcus multilocularis<\/jats:italic>\n                    eggs. Four methods were compared: passive fecal float, centrifugal fecal float, mini-FLOTAC, and the KeyScreen GI PCR Parasite panel (Antech Diagnostics). Domestic dog feces were spiked with 8 egg concentrations (0, 10, 25, 50, 60, 100, 200, and 500 eggs\/g). Each concentration had 10 replicates. Detection frequency (ability to detect \u2265 1 egg) and mean egg recovery were assessed. The centrifugal method had the lowest detection limit, detecting eggs at 10 eggs\/g in 20% of replicates, though mean recovery was low. The mini-FLOTAC demonstrated the highest mean egg recovery and overall accuracy (9.01%) among quantitative methods. Passive float and KeyScreen detected eggs consistently at \u2265 60 eggs\/g, with KeyScreen showing higher detection frequency than passive floats. Importantly, KeyScreen is the only method that can distinguish\n                    <jats:italic>Echinococcus<\/jats:italic>\n                    species from the more common\n                    <jats:italic>Taenia<\/jats:italic>\n                    species. The mini-FLOTAC is practical for veterinary offices, particularly where centrifugation is unavailable, offering efficient\n                    <jats:italic>Echinococcus<\/jats:italic>\n                    egg recovery. KeyScreen provides species-level confirmation while detecting low-concentration infections. This is particularly important in areas where\n                    <jats:italic>Echinococcus<\/jats:italic>\n                    species are emerging and zoonotic risk is a concern. Accurate diagnostic methods are essential for veterinary practice and One Health surveillance, and all dogs should be regularly tested and treated. The companion Currents in One Health by Garrett et al,\n                    <jats:italic>JAVMA<\/jats:italic>\n                    , November 2026, addresses the natural history of the 2 most common\n                    <jats:italic>Echinococcus<\/jats:italic>\n                    species of concern in North America,\n                    <jats:italic>E multilocularis<\/jats:italic>\n                    and\n                    <jats:italic>Echinococcus canadensis<\/jats:italic>\n                    (G8 and G10).\n                  <\/jats:p>","DOI":"10.2460\/ajvr.26.04.0177","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:57:20Z","timestamp":1783954640000},"source":"Crossref","is-referenced-by-count":0,"title":["Fecal floatation and KeyScreen polymerase chain reaction optimize Echinococcus multilocularis detection in canine feces for veterinary practice and zoonotic surveillance"],"prefix":"10.2460","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7893-6157","authenticated-orcid":true,"given":"Kayla B.","family":"Garrett","sequence":"first","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"Warnell School of Forestry and Natural Resources, University of Georgia, Athens GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2452-5015","authenticated-orcid":true,"given":"Michael J.","family":"Yabsley","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"Warnell School of Forestry and Natural Resources, University of Georgia, Athens GA"},{"name":"Center for Ecology of Infectious Diseases, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sierra R.","family":"Felty","sequence":"additional","affiliation":[{"name":"REU Site Program in the Population Biology of Infectious Diseases, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Braxton T.","family":"Gise","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5596-7380","authenticated-orcid":true,"given":"Pablo","family":"Jimenez Castro","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics, Antech Diagnostics, Loveland, CO"},{"name":"Grupo De Parasitolog\u00eda Veterinaria, Universidad Nacional De Colombia, Bogot\u00e1, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Leutenegger","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics, Antech Diagnostics, Loveland, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Loo","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics, Antech Diagnostics, Loveland, CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Newton","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA"},{"name":"College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5437-4011","authenticated-orcid":true,"given":"Christopher A.","family":"Cleveland","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"2","key":"B1","series-title":"Emerg Infect Dis","first-page":"230","article-title":"Echinococcus spp. tapeworms in North America","volume":"24","author":"Cerda JR","year":"2018","unstructured":"Cerda JR, Buttke DE, Ballweber LR. 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