{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:26:03Z","timestamp":1785522363764,"version":"3.56.0"},"reference-count":29,"publisher":"American Veterinary Medical Association (AVMA)","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"sc_a1\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To characterize the total and inorganic serum iodine concentrations of canines with varying thyroid disease status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a2\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Leftover serum samples submitted to the Michigan State University Veterinary Diagnostic Laboratory for diagnostic thyroid panel screening between January and June 2023 were utilized for this retrospective study. Thyroglobulin autoantibody was used as a marker for autoimmune thyroiditis. Inorganic and total iodine were measured via an inductively coupled plasma\u2013mass spectrometer. An estimated reference interval was calculated for inorganic and total iodine using results from euthyroid dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a3\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Iodine concentrations were measured in dogs with autoimmune thyroiditis (n = 101) and hypothyroidism (97) and euthyroid dogs (86). Serum iodine concentrations ranged widely but were not increased in dogs with autoimmune thyroiditis or hypothyroidism in comparison to euthyroid animals. Concentrations of both total and inorganic iodine were statistically different between dogs suffering from autoimmune thyroiditis and those with hypothyroidism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a4\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Elevated serum iodine is not associated with autoimmune thyroiditis or hypothyroidism in dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a5\">\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>The concentration of iodine alone in canine serum is not a marker for thyroid disease in dogs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/ajvr.26.05.0204","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T17:14:27Z","timestamp":1785518067000},"page":"1-5","source":"Crossref","is-referenced-by-count":0,"title":["Serum iodine concentrations in euthyroid dogs and dogs with autoimmune thyroiditis and hypothyroidism"],"prefix":"10.2460","author":[{"given":"Rebecca J.","family":"Egbert","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology and Diagnostic Information, College of Veterinary Medicine, Michigan State University, Lansing, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Buchweitz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology and Diagnostic Information, College of Veterinary Medicine, Michigan State University, Lansing, MI"},{"name":"Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University, Lansing, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison A.","family":"Godfrey","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology and Diagnostic Information, College of Veterinary Medicine, Michigan State University, Lansing, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian K.","family":"Petroff","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology and Diagnostic Information, College of Veterinary Medicine, Michigan State University, Lansing, MI"},{"name":"Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Michigan State University, Lansing, MI"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"B1","series-title":"Endotext","article-title":"Chapter 2: thyroid hormone synthesis and secretion","author":"Miot F","year":"2000","unstructured":"Miot F, Dupuy C, Dumont J, Rousset B. 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Immunol Res. 2021;69:129\u2013138. doi:10.1007\/s12026-021-09192-6"},{"issue":"3","key":"B6","series-title":"J Clin Endocrinol Metab","first-page":"1031","article-title":"The effect of voluntary iodine prophylaxis in a small rural community: the pescopagano survey 15 years later","volume":"98","author":"Aghini Lombardi F","year":"2013","unstructured":"Aghini Lombardi F, Fiore E, Tonacchera M, et al. The effect of voluntary iodine prophylaxis in a small rural community: the pescopagano survey 15 years later. J Clin Endocrinol Metab. 2013;98(3):1031\u20131039. doi:10.1210\/jc.2012-2960"},{"issue":"1","key":"B7","series-title":"Thyroid","first-page":"118","article-title":"Impact of nutritional iodine optimization on rates of thyroid hypoechogenicity and autoimmune thyroiditis: a cross-sectional, comparative study","volume":"25","author":"Miranda DM","year":"2015","unstructured":"Miranda DM, Massom JN, Catarino RM, et al. 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