{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:06:26Z","timestamp":1790697986870,"version":"4.1.0"},"reference-count":32,"publisher":"American Veterinary Medical Association (AVMA)","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"sc_a1\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>African swine fever is a devastating swine disease lacking effective vaccines or antiviral drugs. The African swine fever virus (ASFV) pE165R deoxyuridine triphosphatase (dUTPase), critical for viral replication, is a promising antiviral target. Therefore, identifying inhibitors that target the pE165R protein represents a potential antiviral strategy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a2\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The study computationally screened FDA-approved drugs against pE165R\u2019s crystal structure. Molecular dynamics simulations elucidated inhibitor-pE165R interactions, and in vitro enzymatic assays quantitatively assessed inhibitory effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a3\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>4 candidates predominantly targeted pE165R\u2019s active pocket (Serine-72, Arginine-71, and Serine-73), suggesting competitive inhibition, with strong binding free energies (up to \u221240.5 kcal\/mol) observed by molecular mechanics\/Poisson-Boltzmann surface area.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a4\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In vitro assays confirmed dideoxyinosine triphosphate (77.9%) potently inhibited pE165R activity at 0.1mM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a5\">\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>Effective drugs against ASFV remain lacking. Based on a drug-repurposing strategy, this study screened the FDA-approved drug library for compounds targeting pE165R, a key protein involved in ASFV replication. Subsequent pharmacodynamic evaluation is expected to facilitate the identification of affordable and effective anti-ASFV therapeutics for clinical application, thereby providing a theoretical foundation for the effective prevention and control of African swine fever.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/ajvr.26.07.0289","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:36:58Z","timestamp":1790696218000},"page":"1-11","source":"Crossref","is-referenced-by-count":0,"title":["Identification and evaluation of repurposing Food and Drug Administration\u2013approved drugs targeting the pE165R protein of African swine fever virus"],"prefix":"10.2460","author":[{"given":"Weiqi","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China"},{"name":"Contributed equally to this work."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqi","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Contributed equally to this work."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuhui","family":"Chang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Contributed equally to this work."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Geng","sequence":"additional","affiliation":[{"name":"Shenyang Customs Technology Center, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouhua","family":"Feng","sequence":"additional","affiliation":[{"name":"Wens Farming and Animal Husbandry Co Ltd, Chaoyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gen","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Ou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyue","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Wei","sequence":"additional","affiliation":[{"name":"Liaoning Agricultural Development Service Center, The Preventive and Control Center of Animal Disease of Liaoning Province, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuofeng","family":"Yang","sequence":"additional","affiliation":[{"name":"Liaoning Agricultural Development Service Center, The Preventive and Control Center of Animal Disease of Liaoning Province, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinling","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Livestock Infectious Diseases, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"},{"name":"Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"5","key":"B1","series-title":"Viruses","first-page":"103","article-title":"African swine fever virus: a review","volume":"9","author":"Galindo I","year":"2017","unstructured":"Galindo I, Alonso C. 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