{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:00:10Z","timestamp":1790190010144,"version":"4.1.0"},"reference-count":36,"publisher":"American Veterinary Medical Association (AVMA)","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2026,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"sc_a1\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To characterize the timing and between-cow consistency of iron-handling transcript changes after lipopolysaccharide (LPS) exposure in primary bovine monocyte-derived macrophages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a2\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Macrophages from 5 adult Holstein donor cows were exposed to\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      LPS or PBS and profiled by RNA sequencing at 30 minutes and 6 hours in a paired design. The LPS-versus-PBS effects were estimated with cow-blocked negative-binomial models. Per-cow direction was examined, and 4 focal transcripts were compared directionally with a separate published cohort of 8 cattle sampled 48 hours after LPS exposure.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a3\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A treatment-by-time interaction was detected for 5,461 genes. At 6 hours,\n                      <jats:italic>transferrin receptor, STEAP3 metalloreductase<\/jats:italic>\n                      , and\n                      <jats:italic>solute carrier 11A2<\/jats:italic>\n                      transcripts increased by 2.61, 1.46, and 2.47 log\n                      <jats:sub>2<\/jats:sub>\n                      fold, respectively, whereas\n                      <jats:italic>solute carrier 40A1<\/jats:italic>\n                      decreased by 2.24 log\n                      <jats:sub>2<\/jats:sub>\n                      fold. All 4 changes had the same direction in each of the 5 cows. The separate cohort showed the same direction for all 4 transcripts (\n                      <jats:italic>transferrin receptor<\/jats:italic>\n                      in 7 of 8 animals; the other genes in 8 of 8); its different protocol precluded kinetic replication.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a4\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>LPS exposure was associated with a later, cow-consistent pattern of increased iron acquisition\u2013associated transcripts and decreased ferroportin transcript abundance. The study does not establish protein abundance, cellular iron movement, or hepcidin dependence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sc_a5\">\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>These transcript-level measures provide an ex vivo framework for studying variation in bovine macrophage iron-handling responses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2460\/ajvr.26.07.0324","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:07:32Z","timestamp":1790186852000},"page":"1-8","source":"Crossref","is-referenced-by-count":0,"title":["Lipopolysaccharide induces a delayed iron-handling transcript pattern in primary bovine macrophages"],"prefix":"10.2460","author":[{"given":"Kevin","family":"Hall","sequence":"first","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah","family":"Dunston","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1645-0036","authenticated-orcid":true,"given":"Jong Hyuk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL"},{"name":"University of Florida Health Cancer Institute, University of Florida, Gainesville, FL"},{"name":"Center for Inflammation and Mucosal Immunology, University of Florida, Gainesville, FL"},{"name":"Artificial Intelligence Academic Initiative Center, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Harvey","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2062-6978","authenticated-orcid":true,"given":"Cleverson","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, FL"},{"name":"University of Florida Health Cancer Institute, University of Florida, Gainesville, FL"},{"name":"Artificial Intelligence Academic Initiative Center, University of Florida, Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"issue":"8","key":"B1","series-title":"Nat Rev Microbiol","first-page":"525","article-title":"Nutritional immunity: transition metals at the pathogen-host interface","volume":"10","author":"Hood MI","year":"2012","unstructured":"Hood MI, Skaar EP. Nutritional immunity: transition metals at the pathogen-host interface. Nat Rev Microbiol. 2012;10(8):525\u2013537. doi:10.1038\/nrmicro2836"},{"key":"B2","series-title":"Mol Aspects Med","first-page":"100864","article-title":"Iron in infection and immunity","author":"Nairz M","year":"2020","unstructured":"Nairz M, Weiss G. Iron in infection and immunity. Mol Aspects Med. 2020;75:100864. doi:10.1016\/j.mam.2020.100864"},{"issue":"8","key":"B3","series-title":"Nat Rev Immunol","first-page":"500","article-title":"Iron homeostasis in host defence and inflammation","volume":"15","author":"Ganz T","year":"2015","unstructured":"Ganz T, Nemeth E. Iron homeostasis in host defence and inflammation. Nat Rev Immunol. 2015;15(8):500\u2013510. doi:10.1038\/nri3863"},{"issue":"3","key":"B4","series-title":"Immunity","first-page":"492","article-title":"Macrophages and iron metabolism","volume":"44","author":"Soares MP","year":"2016","unstructured":"Soares MP, Hamza I. Macrophages and iron metabolism. Immunity. 2016;44(3):492\u2013504. doi:10.1016\/j.immuni.2016.02.016"},{"issue":"9","key":"B5","series-title":"Cell Microbiol","first-page":"2126","article-title":"The co-ordinated regulation of iron homeostasis in murine macrophages limits the availability of iron for intracellular Salmonella typhimurium","volume":"9","author":"Nairz M","year":"2007","unstructured":"Nairz M, Theurl I, Ludwiczek S, et al. The co-ordinated regulation of iron homeostasis in murine macrophages limits the availability of iron for intracellular Salmonella typhimurium. Cell Microbiol. 2007;9(9):2126\u20132140. doi:10.1111\/j.1462-5822.2007.00942.x"},{"issue":"22","key":"B6","series-title":"Eur J Biochem","first-page":"6586","article-title":"Interferon-gamma and lipopolysaccharide regulate the expression of Nramp2 and increase the uptake of iron from low relative molecular mass complexes by macrophages","volume":"267","author":"Wardrop SL","year":"2000","unstructured":"Wardrop SL, Richardson DR. Interferon-gamma and lipopolysaccharide regulate the expression of Nramp2 and increase the uptake of iron from low relative molecular mass complexes by macrophages. Eur J Biochem. 2000;267(22):6586\u20136593. doi:10.1046\/j.1432-1327.2000.01752.x"},{"issue":"9","key":"B7","series-title":"J Biol Chem","first-page":"6220","article-title":"Effects of interferon-gamma and lipopolysaccharide on macrophage iron metabolism are mediated by nitric oxide-induced degradation of iron regulatory protein 2","volume":"275","author":"Kim S","year":"2000","unstructured":"Kim S, Ponka P. Effects of interferon-gamma and lipopolysaccharide on macrophage iron metabolism are mediated by nitric oxide-induced degradation of iron regulatory protein 2. J Biol Chem. 2000;275(9):6220\u20136226. doi:10.1074\/jbc.275.9.6220"},{"issue":"11","key":"B8","series-title":"Nat Genet","first-page":"1264","article-title":"Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells","volume":"37","author":"Ohgami RS","year":"2005","unstructured":"Ohgami RS, Campagna DR, Greer EL, et al. Identification of a ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Nat Genet. 2005;37(11):1264\u20131269. doi:10.1038\/ng1658"},{"issue":"1","key":"B9","series-title":"PLoS One","first-page":"e0169531","article-title":"Zinc modulates endotoxin-induced human macrophage inflammation through ZIP8 induction and C\/EBP\u03b2 inhibition","volume":"12","author":"Pyle CJ","year":"2017","unstructured":"Pyle CJ, Akhter S, Bao S, Dodd CE, Schlesinger LS, Knoell DL. Zinc modulates endotoxin-induced human macrophage inflammation through ZIP8 induction and C\/EBP\u03b2 inhibition. 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Int J Toxicol. 2014;33(3):246\u2013258. doi:10.1177\/1091581814529310"},{"issue":"30","key":"B11","series-title":"J Biol Chem","first-page":"20674","article-title":"Role of HIF-1 and NF-kappaB transcription factors in the modulation of transferrin receptor by inflammatory and anti-inflammatory signals","volume":"283","author":"Tacchini L","year":"2008","unstructured":"Tacchini L, Gammella E, De Ponti C, Recalcati S, Cairo G. Role of HIF-1 and NF-kappaB transcription factors in the modulation of transferrin receptor by inflammatory and anti-inflammatory signals. J Biol Chem. 2008;283(30):20674\u201320686. doi:10.1074\/jbc.M800365200"},{"issue":"3","key":"B12","series-title":"Eur J Immunol","first-page":"824","article-title":"Differential regulation of iron homeostasis during human macrophage polarized activation","volume":"40","author":"Recalcati S","year":"2010","unstructured":"Recalcati S, Locati M, Marini A, et al. 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J R Stat Soc Series B Stat Methodol. 1995;57(1):289\u2013300. doi:10.1111\/j.2517-6161.1995.tb02031.x"},{"issue":"11","key":"B27","series-title":"PLoS Comput Biol","first-page":"e1000217","article-title":"Inferring pathway activity toward precise disease classification","volume":"4","author":"Lee E","year":"2008","unstructured":"Lee E, Chuang HY, Kim JW, Ideker T, Lee D. Inferring pathway activity toward precise disease classification. PLoS Comput Biol. 2008;4(11):e1000217. doi:10.1371\/journal.pcbi.1000217"},{"key":"B28","series-title":"bioRxiv","article-title":"Fast gene set enrichment analysis","author":"Korotkevich G","year":"2021","unstructured":"Korotkevich G, Sukhov V, Budin N, Shpak B, Artyomov MN, Sergushichev A. Fast gene set enrichment analysis. Posted online February 1, 2021. bioRxiv. doi:10.1101\/060012"},{"issue":"43","key":"B29","series-title":"Proc Natl Acad Sci U S A","first-page":"15545","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian A","year":"2005","unstructured":"Subramanian A, Tamayo P, Mootha VK, et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci U S A. 2005;102(43):15545\u201315550. doi:10.1073\/pnas.0506580102"},{"issue":"7","key":"B30","series-title":"J Immunol","first-page":"4408","article-title":"Lipopolysaccharide-induced heme oxygenase-1 expression in human monocytic cells is mediated via Nrf2 and protein kinase C","volume":"175","author":"Rushworth SA","year":"2005","unstructured":"Rushworth SA, Chen XL, Mackman N, Ogborne RM, O\u2019Connell MA. 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