{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T22:12:54Z","timestamp":1775167974726,"version":"3.50.1"},"reference-count":52,"publisher":"American Veterinary Medical Association (AVMA)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,1,1]]},"abstract":"Abstract<\/jats:title>\nObjective\u2014<\/jats:bold>To investigate the distribution of mRNA coding for 7 subtypes of 5-hydroxytryptamine receptors (5-HTRs) in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation (CDD).<\/jats:p>\nSample Population\u2014<\/jats:bold>Full-thickness intestinal wall biopsy specimens were obtained from the ileum, cecum, proximal loop of the ascending colon, and external loop of the spiral colon (ELSC) of 15 cows with CDD (group 1) and 15 healthy dairy cows allocated to 2 control groups (specimens collected during routine laparotomy [group 2] or after cows were slaughtered [group 3]).<\/jats:p>\nProcedure\u2014<\/jats:bold>Amounts of mRNA coding for 7 subtypes of 5-HTRs (5-HT1A<\/jats:sub>, 5-HT1B<\/jats:sub>, 5-HT1D<\/jats:sub>, 5-HT1F<\/jats:sub>, 5-HT2A<\/jats:sub>, 5-HT2B<\/jats:sub>, and 5-HT4<\/jats:sub>) were measured by quantitative real-time reverse transcriptase\u2013PCR assay. Results were expressed as the percentage of mRNA expression of a housekeeping gene.<\/jats:p>\nResults\u2014<\/jats:bold>Expression of mRNA coding for 5-HTR1B<\/jats:sub>, 5-HTR2B<\/jats:sub>, and 5-HTR4<\/jats:sub> was significantly lower in cows with CDD than in healthy cows. For 5-HTR2B<\/jats:sub> and 5-HTR4<\/jats:sub>, significant differences between cows with CDD and control cows were most pronounced for the ELSC. Expression of mRNA for 5-HTR1D<\/jats:sub>, 5-HTR1F<\/jats:sub>, and 5-HTR2A<\/jats:sub> was extremely low in all groups, and mRNA for 5-HTR1A<\/jats:sub> was not detected.<\/jats:p>\nConclusions and Clinical Relevance\u2014<\/jats:bold>Relative concentrations of mRNA coding for 5-HTR1B<\/jats:sub>, 5-HT2B<\/jats:sub>, and 5-HTR4<\/jats:sub> were significantly lower in the intestines of cows with CDD than in the intestines of healthy dairy cows, especially for 5-HT2B<\/jats:sub> and 5-HTR4<\/jats:sub> in the ELSC. This supports the hypothesis that serotonergic mechanisms, primarily in the spiral colon, are implicated in the pathogenesis of CDD.<\/jats:p>","DOI":"10.2460\/ajvr.67.1.95","type":"journal-article","created":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T22:24:51Z","timestamp":1137795891000},"page":"95-101","source":"Crossref","is-referenced-by-count":13,"title":["Distribution of mRNA coding for 5-hydroxytryptamine receptor subtypes in the intestines of healthy dairy cows and dairy cows with cecal dilatation-dislocation"],"prefix":"10.2460","volume":"67","author":[{"given":"Ladina","family":"Engel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Kobel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar C.","family":"Ontsouka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans U.","family":"Graber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg W.","family":"Blum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Steiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mireille","family":"Meylan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Schweizer Archiv Tierheilkd","first-page":"423","article-title":"Epidemiologie und Risikofaktoren der Blinddarmdilatation und Labmagenverlagerung bei der Milchkuh","volume":"141","author":"Eicher","year":"1999"},{"key":"ref2","series-title":"Vet Rec","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1136\/vr.125.10.265","article-title":"Clinical findings in cattle with dilatation and torsion of the 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