{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:10:39Z","timestamp":1762917039020,"version":"3.38.0"},"reference-count":33,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine the immunogenicity and efficacy of <jats:italic>Brucella abortus<\/jats:italic> strain RB51 (SRB51) as a vaccine in domestic pigs.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u2014Sixty-eight 6-week-old crossbred domestic pigs and twenty-four 4-month-old gilts.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014In experiment 1, pigs were vaccinated IM (n = 51) with 2 \u00d7 10<jats:sup>10<\/jats:sup> CFUs of SRB51 or sham inoculated (17). Periodic blood samples were obtained to perform blood cultures, serologic evaluations, and cell-mediated immunity assays. Necropsies were performed at selected times between weeks 1 and 23 after vaccination to determine vaccine clearance. In experiment 2, gilts were similarly vaccinated (n = 18) or sham inoculated (8) and similar samples were obtained after vaccination. Gilts were bred and challenged conjunctivally with 5.0 \u00d7 10<jats:sup>7<\/jats:sup> CFUs of virulent <jats:italic>Brucella suis<\/jats:italic> strain 3B. Necropsies were performed on gilts and on fetuses or neonates after abortion or parturition, respectively. Bacterial cultures and serologic evaluations were performed on samples obtained at necropsy to determine vaccine efficacy.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Humoral and cell-mediated immune responses did not differ between vaccinates and controls. After vaccination, SRB51 was not isolated from blood cultures of either group and was isolated from lymphoid tissues of 3 pigs at 2 weeks (n = 2) and 4 weeks (1) after vaccination. No differences were found in isolation of B suis or in seroconversion between vaccinated and control gilts and between their neonates or aborted fetuses.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Parenteral vaccination with SRB51 does not induce humoral or cell-mediated immune responses. Vaccination with SRB51 did not protect gilts or their neonates and fetuses from virulent challenge with <jats:italic>B suis<\/jats:italic>.<\/jats:p>","DOI":"10.2460\/ajvr.67.10.1802","type":"journal-article","created":{"date-parts":[[2006,10,2]],"date-time":"2006-10-02T13:49:29Z","timestamp":1159796969000},"page":"1802-1808","source":"Crossref","is-referenced-by-count":18,"title":["Parenteral vaccination of domestic pigs with Brucella abortus strain RB51"],"prefix":"10.2460","volume":"67","author":[{"given":"William C.","family":"Stoffregen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven C.","family":"Olsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betsy J.","family":"Bricker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Diseases of swine","first-page":"603","article-title":"Brucellosis","author":"MacMillan","year":"2006"},{"key":"ref2","series-title":"J Am Vet Med Assoc","first-page":"640","article-title":"Immunology and public health significance of swine brucellosis","volume":"160","author":"Deyoe","year":"1972"},{"key":"ref3","series-title":"Proc Annu Meet U S Anim Health Assoc","first-page":"102","article-title":"Sites of localization of Brucella suis in swine","volume":"71","author":"Deyoe","year":"1967"},{"key":"ref4","series-title":"J S C Med Assoc","first-page":"113","article-title":"Brucellosis in two hunt club members in South Carolina","volume":"100","author":"Starnes","year":"2004"},{"key":"ref5","series-title":"J Occup Environ Med","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1097\/00043764-199506000-00011","article-title":"Outbreak of brucellosis at a United States pork packing plant","volume":"37","author":"Trout","year":"1995"},{"series-title":"Veterinary Services. 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