{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T08:58:17Z","timestamp":1780736297393,"version":"3.54.1"},"reference-count":48,"publisher":"American Veterinary Medical Association (AVMA)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine whether particular antimicrobial susceptibility profiles of bovine mastitis-causing <jats:italic>Staphylococcus aureus<\/jats:italic> isolates were associated with specific <jats:italic>S aureus<\/jats:italic> genotypes.<\/jats:p>\n<jats:p><jats:bold>Sample Population<\/jats:bold>\u2014357 <jats:italic>S aureus<\/jats:italic> isolates recovered from milk samples submitted for diagnostic bacteriologic testing from 24 dairy herds.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Antimicrobial susceptibility of <jats:italic>S aureus<\/jats:italic> isolates was assessed by determining minimum inhibitory concentrations (MICs) to 14 antimicrobial agents. After digestion of <jats:italic>S aureus<\/jats:italic> genomic DNA by <jats:italic>Sma<\/jats:italic>I, electrophoretic patterns were obtained via pulsed-field gel electrophoresis (PFGE) and used to classify isolates into types. Gels were analyzed, and data were used to prepare dendrograms.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014308 of 357 (86%) <jats:italic>S aureus<\/jats:italic> isolates were susceptible to all antimicrobials evaluated. Forty-nine <jats:italic>S aureus<\/jats:italic> isolates were resistant to 1 or more antimicrobials; of these isolates, 37 were resistant only to penicillin, 9 were resistant to penicillin and erythromycin, 2 were resistant to tetracycline, and 1 was resistant to erythromycin. Isolates were assigned to 7 PFGE types. An association was found between PFGE type and antimicrobial susceptibility profile. Organisms with resistance to at least one of the tested antimicrobial agents were identified in only 4 of the 7 types of <jats:italic>S aureus<\/jats:italic>.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Antimicrobial resistance was uncommon among the mastitis-causing <jats:italic>S aureus<\/jats:italic> isolates identified in the milk samples. A limited number of genotypes were associated with mastitis in these herds. Antimicrobial resistance phenotypes were associated with particular <jats:italic>S aureus<\/jats:italic> PFGE types; this association may have implications for future treatment and control of <jats:italic>S aureus<\/jats:italic>\u2013associated mastitis in cattle.<\/jats:p>","DOI":"10.2460\/ajvr.67.7.1185","type":"journal-article","created":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T16:45:30Z","timestamp":1152204330000},"page":"1185-1191","source":"Crossref","is-referenced-by-count":41,"title":["Genetic diversity and antimicrobial susceptibility profiles among mastitis-causing Staphylococcus aureus isolated from bovine milk samples"],"prefix":"10.2460","volume":"67","author":[{"given":"Kevin L.","family":"Anderson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta L.","family":"Lyman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonya M.","family":"Bodeis-Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Staphylococcus aureus infection and disease","first-page":"271","article-title":"Staphylococcus aureus mastitis","author":"Fox","year":"2001"},{"key":"ref2"},{"key":"ref3","series-title":"Vet Res","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1051\/vetres:2001129","article-title":"Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus and canine Staphylococcus intermedius","volume":"32","author":"Werckenthin","year":"2001"},{"key":"ref4","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.2460\/javma.2003.222.1582","article-title":"Antimicrobial resistance of bacteria isolated from dairy cow milk samples submitted for bacterial culture: 8,905 samples (1994\u20132001)","volume":"222","author":"Makovec","year":"2003"},{"key":"ref5","series-title":"J Dairy Sci","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.3168\/jds.S0022-0302(02)74172-6","article-title":"Trends in antibacterial susceptibility of mastitis pathogens during a seven-year period","volume":"85","author":"Erskine","year":"2002"},{"key":"ref6","series-title":"43rd Annu Meet Natl Mast Counc","first-page":"400","article-title":"Bovine mastitis pathogens and trends in resistance to antibacterial drugs. 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