{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:23:55Z","timestamp":1762079035389,"version":"3.38.0"},"reference-count":33,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To evaluate whether biochemical or genetic alterations in AMP-activated protein kinase (AMPK) play a role in the development of polysaccharide storage myopathy (PSSM) in Quarter Horses.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u201430 PSSM-affected and 30 unaffected (control) Quarter Horses.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014By use of an established peptide phosphotransfer assay, basal and maximal AMPK activities were measured in muscle biopsy samples obtained from 6 PSSM-affected and 6 control horses. In 24 PSSM-affected and 24 control horses, microsatellite markers identified from the chromosomal locations of all 7 AMPK subunit genes were genotyped with a fluorescent DNA fragment analyzer. Alleles of 2 of the AMPK \u03b3 subunit genes were genotyped via DNA sequencing. Allele frequencies of DNA markers in or near the AMPK subunit genes were measured in isolated genomic DNA.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014No differences in basal or maximal muscle AMPK enzyme activities between PSSM-affected and control horses were detected. There were also no differences in allele frequencies for microsatellite markers near any of the 7 AMPK subunit genes between the 2 groups. Furthermore, previously known and newly identified alleles of 2 equine AMPK \u03b3 subunit genes were also not associated with PSSM.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014These results have provided no evidence to indicate that AMPK plays a causative role in PSSM in American Quarter Horses.<\/jats:p>","DOI":"10.2460\/ajvr.68.10.1079","type":"journal-article","created":{"date-parts":[[2008,3,21]],"date-time":"2008-03-21T17:39:46Z","timestamp":1206121186000},"page":"1079-1084","source":"Crossref","is-referenced-by-count":7,"title":["Biochemical and genetic evaluation of the role of AMP-activated protein kinase in polysaccharide storage myopathy in Quarter Horses"],"prefix":"10.2460","volume":"68","author":[{"given":"Patricia K.","family":"Dranchak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiona C.","family":"Leiper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie J.","family":"Valberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Piercy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Carling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly E.","family":"McCue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James R.","family":"Mickelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Neuromuscul Disord","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/S0960-8966(06)80006-4","article-title":"Polysaccharide storage myopathy associated with recurrent exertional rhabdomyolysis in horses","volume":"2","author":"Valberg","year":"1992"},{"key":"ref2","series-title":"Compend Contin Educ Pract Vet","first-page":"1077","article-title":"Exertional rhabdomyolysis and polysaccharide storage myopathy in horses","volume":"19","author":"Valberg","year":"1997"},{"key":"ref3","series-title":"Neuromuscul Disord","first-page":"666","article-title":"Insulin sensitivity and skeletal muscle glucose transport in horses with equine polysaccharide storage myopathy","volume":"10","author":"Annadale","year":"2004"},{"key":"ref4","series-title":"Am J Vet Res","first-page":"286","article-title":"Familial basis of exertional rhabdomyolysis in quarter horse-related breeds","volume":"57","author":"Valberg","year":"1996"},{"key":"ref5","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1111\/j.1939-1676.2002.tb02391.x","article-title":"Developmental onset of polysaccharide storage myopathy in 4 Quarter Horse foals","volume":"16","author":"De La Corte","year":"2002"},{"key":"ref6","series-title":"Muscle Nerve","first-page":"S10","article-title":"Biochemistry and molecular genetics of human glycogenoses: an overview","volume":"3","author":"DiMauro","year":"1995"},{"key":"ref7","series-title":"Am J Vet Res","first-page":"782","article-title":"Skeletal muscle glycolytic capacity and phosphofructokinase regulation in horses with polysaccharide storage myopathy","volume":"59","author":"Valberg","year":"1998"},{"key":"ref8","series-title":"Equine Vet J","first-page":"43","article-title":"Skeletal muscle metabolic response to exercise in horses with \u2018tying-up\u2019 due to polysaccharide storage myopathy","volume":"1","author":"Valberg","year":"1999"},{"key":"ref9","series-title":"Am J Vet Res","first-page":"458","article-title":"Glucose uptake in horses with polysaccharide storage myopathy","volume":"60","author":"De La Corte","year":"1999"},{"key":"ref10","series-title":"Equine Vet J","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1111\/j.2042-3306.1997.tb01672.x","article-title":"Severe polysaccharide storage myopathy in Belgian and Percheron draught horses","volume":"29","author":"Valentine","year":"1997"},{"key":"ref11","series-title":"J Vet Diagn Invest","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1177\/104063870101300112","article-title":"Incidence of polysaccharide storage myopathy in draft horse-related breeds: a necropsy study of 37 horses and a mule","volume":"13","author":"Valentine","year":"2001"},{"key":"ref12","series-title":"Vet Pathol","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1354\/vp.42-6-823","article-title":"Incidence of polysaccharide storage myopathy: necropsy study of 225 horses","volume":"42","author":"Valentine","year":"2005"},{"key":"ref13","series-title":"Equine Vet J Suppl","first-page":"340","article-title":"Prevalence of polysaccharide storage myopathy in horses with neuromuscular disorders","volume":"36","author":"McCue","year":"2006"},{"key":"ref14","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1958","DOI":"10.2460\/javma.2005.227.1958","article-title":"Prevalences and clinical signs of polysaccharide storage myopathy and shivers in Belgian draft horses","volume":"227","author":"Firshman","year":"2005"},{"key":"ref15","series-title":"Vet Pathol","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1354\/vp.43-3-257","article-title":"Comparison of histopathologic criteria and skeletal muscle fixation techniques for the diagnosis of polysaccharide storage myopathy in horses","volume":"43","author":"Firshman","year":"2006"},{"key":"ref16","series-title":"Endocrinology","doi-asserted-by":"publisher","first-page":"5179","DOI":"10.1210\/en.2003-0982","article-title":"Minireview: the AMP-activated protein kinase cascade: the key sensor of cellular energy status","volume":"144","author":"Hardie","year":"2003"},{"key":"ref17","series-title":"Proc Natl Acad Sci U S A","doi-asserted-by":"publisher","first-page":"8839","DOI":"10.1073\/pnas.1533136100","article-title":"Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases","volume":"100","author":"Hong","year":"2003"},{"key":"ref18","series-title":"Cell Metab","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.cmet.2005.06.005","article-title":"Ca2+\/calmodulindependent protein kinase kinase-beta acts upstream of AMP-activated protein kinase in mammalian cells","volume":"2","author":"Woods","year":"2005"},{"key":"ref19","series-title":"Am J Physiol Endocrinol Metab","doi-asserted-by":"publisher","first-page":"E194","DOI":"10.1152\/ajpendo.00147.2003","article-title":"Expression profiling of the gamma-subunit isoforms of AMP-activated protein kinase suggests a major role for gamma3 in white skeletal muscle","volume":"286","author":"Mahlapuu","year":"2004"},{"key":"ref20","series-title":"J Clin Invest","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1172\/JCI0214571","article-title":"Constitutively active AMP kinase mutations cause glycogen storage disease mimicking hypertrophic cardiomyopathy","volume":"109","author":"Arad","year":"2002"},{"key":"ref21","series-title":"Science","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1126\/science.288.5469.1248","article-title":"A mutation in PRKAG3 associated with excess glycogen content in pig skeletal muscle","volume":"288","author":"Milan","year":"2000"},{"key":"ref22","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"27879","DOI":"10.1074\/jbc.271.44.27879","article-title":"Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase","volume":"271","author":"Hawley","year":"1996"},{"key":"ref23","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"10282","DOI":"10.1074\/jbc.271.17.10282","article-title":"Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro","volume":"271","author":"Woods","year":"1996"},{"key":"ref24","series-title":"Eur J Biochem","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1111\/j.1432-1033.1989.tb15185.x","article-title":"Tissue distribution of the AMP-activated protein kinase, and lack of activation by cyclic-AMP-dependent protein kinase, studied using a specific and sensitive peptide assay","volume":"186","author":"Davies","year":"1989"},{"key":"ref25","series-title":"Anim Genet","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1111\/j.1365-2052.2006.01431.x","article-title":"Chromosomal assignments for the equine AMPK family genes","volume":"37","author":"Dranchak","year":"2006"},{"key":"ref26","series-title":"Am J Hum Genet","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1086\/316944","article-title":"Extent and distribution of linkage disequilibrium in three genomic regions","volume":"68","author":"Abecasis","year":"2001"},{"key":"ref27","series-title":"Stroke","doi-asserted-by":"publisher","first-page":"2064","DOI":"10.1161\/01.STR.0000139380.50649.5c","article-title":"Association of polymorphisms and haplotypes in the elastin gene in Dutch patients with sporadic aneurysmal subarachnoid hemorrhage","volume":"35","author":"Ruigrok","year":"2004"},{"key":"ref28","series-title":"Genome Res","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1101\/gr.917503","article-title":"The first generation whole genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes","volume":"13","author":"Chowdhary","year":"2003"},{"key":"ref29","series-title":"Genomics","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ygeno.2005.09.001","article-title":"Single linkage group per chromosome genetic linkage map for the horse, based on two, three-generation, full-sibling, cross-bred horse reference families","volume":"87","author":"Swinburne","year":"2006"},{"key":"ref30","series-title":"Cytogenet Genome Res","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1159\/000075751","article-title":"Molecular characterization and mutational screening of the PRKAG3 gene in the horse","volume":"102","author":"Park","year":"2003"},{"key":"ref31","series-title":"Biochem Soc Trans","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1042\/bst0310232","article-title":"Identification and characterization of AMPK gamma 3 mutations in the pig","volume":"31","author":"Andersson","year":"2003"},{"key":"ref32","series-title":"Eur J Biochem","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1111\/j.1432-1033.1995.tb20255.x","article-title":"The AMP-activated protein kinase gene is highly expressed in rat skeletal muscle. Alternative splicing and tissue distribution of the mRNA","volume":"228","author":"Verhoeven","year":"1995"},{"key":"ref33","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"12443","DOI":"10.1074\/jbc.273.20.12443","article-title":"Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle","volume":"273","author":"Thornton","year":"1998"}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/68\/10\/ajvr.68.10.1079.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/68\/10\/ajvr.68.10.1079.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/68\/10\/ajvr.68.10.1079.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T12:16:10Z","timestamp":1741349770000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/68\/10\/ajvr.68.10.1079.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":33,"journal-issue":{"issue":"10"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.68.10.1079","relation":{},"ISSN":["0002-9645"],"issn-type":[{"type":"print","value":"0002-9645"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}