{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T04:56:54Z","timestamp":1778043414513,"version":"3.51.4"},"reference-count":55,"publisher":"American Veterinary Medical Association (AVMA)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To analyze the expression of versican and hyaluronan in melanocytomas and malignant melanomas of dogs, to correlate their expression with expression of the hyaluronan receptor CD44, and to identify enzymes responsible for the synthesis and degradation of hyaluronan in canine dermal fibroblasts and canine melanoma cell lines.<\/jats:p>\n<jats:p><jats:bold>Sample Population<\/jats:bold>\u201435 biopsy specimens from melanocytic tumors of dogs, canine primary dermal fibroblasts, and 3 canine melanoma cell lines.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Versican, hyaluronan, and CD44 were detected in tumor samples by use of histochemical or immunohistochemical methods. Expression of hyaluronan-metabolizing enzymes was analyzed with a reverse transcriptase\u2013PCR assay.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Versican was found only in some hair follicles and around some blood vessels in normal canine skin, whereas hyaluronan was primarily found within the dermis. Hyaluronan was found in connective tissue of the oral mucosa. Versican and, to a lesser extent, hyaluronan were significantly overexpressed in malignant melanomas, compared with expression in melanocytomas. No significant difference was found between malignant tumors from oral or cutaneous origin. The expression of both molecules was correlated, but hyaluronan had a more extensive distribution than versican. Versican and hyaluronan were mainly associated with tumor stroma. Canine fibroblasts and melanoma cell lines expressed hyaluronan synthase 2 and 3 (but not 1) and hyaluronidase 1 and 2.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Versican may be useful as a diagnostic marker for melanocytic tumors in dogs. Knowledge of the enzymes involved in hyaluronan metabolism could reveal new potential therapeutic targets.<\/jats:p>","DOI":"10.2460\/ajvr.68.12.1376","type":"journal-article","created":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T14:16:45Z","timestamp":1196518605000},"page":"1376-1385","source":"Crossref","is-referenced-by-count":9,"title":["Altered expression of versican and hyaluronan in melanocytic tumors of dogs"],"prefix":"10.2460","volume":"68","author":[{"given":"Mar\u00eda-Jos\u00e9","family":"Docampo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa M.","family":"Rabanal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laia","family":"Miquel-Serra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clelia","family":"Domenzain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bassols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Vet Pathol","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1354\/vp.39-6-651","article-title":"A comparative review of melanocytic neoplasms","volume":"39","author":"Smith","year":"2002"},{"key":"ref2","series-title":"Vet Pathol","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1354\/vp.43-2-136","article-title":"The histologic and epidemiologic bases for prognostic considerations in canine melanocytic neoplasia","volume":"43","author":"Spangler","year":"2006"},{"key":"ref3","series-title":"Annu Rev Pathol Mech Dis","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1146\/annurev.pathol.1.110304.100224","article-title":"Tumor stroma and regulation of cancer development","volume":"1","author":"Tlsty","year":"2006"},{"key":"ref4","series-title":"Crit Rev Oncol Hematol","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.critrevonc.2003.10.007","article-title":"Stromal reaction in cutaneous melanoma","volume":"49","author":"Labrousse","year":"2004"},{"key":"ref5","series-title":"Cell Mol Life Sci","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1007\/PL00000690","article-title":"Lecticans: organizers of the brain extracellular matrix","volume":"57","author":"Yamaguchi","year":"2000"},{"key":"ref6","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"5435","DOI":"10.1074\/jbc.M411297200","article-title":"Expression and purification of functionally active hyaluronan-binding domains from human cartilage link protein, aggrecan and versican: formation of ternary complexes with defined hyaluronan oligosaccharides","volume":"280","author":"Seyfried","year":"2005"},{"key":"ref7","series-title":"Glycobiology","doi-asserted-by":"publisher","first-page":"37R","DOI":"10.1093\/glycob\/12.3.37R","article-title":"Hyaluronan promotes the malignant phenotype","volume":"12","author":"Toole","year":"2002"},{"key":"ref8","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"4593","DOI":"10.1074\/jbc.R100039200","article-title":"Hyaluronan-cell interactions in cancer and vascular disease","volume":"277","author":"Toole","year":"2002"},{"key":"ref9","series-title":"Am J Pathol","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1016\/S0002-9440(10)64232-0","article-title":"Hyaluronan and tumor growth","volume":"161","author":"Toole","year":"2002"},{"key":"ref10","series-title":"Proteoglycans: structure, biology and molecular interactions","first-page":"327","article-title":"Versican","author":"Zimmermann","year":"2000"},{"key":"ref11","series-title":"Curr Opin Cell Biol","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/S0955-0674(02)00375-7","article-title":"Versican: a versatile extracellular matrix proteoglycan in cell biology","volume":"14","author":"Wight","year":"2002"},{"key":"ref12","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"10003","DOI":"10.1016\/S0021-9258(19)50191-0","article-title":"Hyaluronate binding properties of versican","volume":"267","author":"LeBaron","year":"1992"},{"key":"ref13","series-title":"Am J Pathol","first-page":"1721","article-title":"Tumor-associated hyaluronan. 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