{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:16:52Z","timestamp":1770481012625,"version":"3.49.0"},"reference-count":38,"publisher":"American Veterinary Medical Association (AVMA)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2008,1,1]]},"abstract":"Abstract<\/jats:title>\nObjective<\/jats:bold>\u2014To compare biomaterials used in orthopedics with respect to in vitro cell viability and cell retention and to in vivo tissue healing and regeneration.<\/jats:p>\nAnimals<\/jats:bold>\u201465 adult female Sprague-Dawley rats and synovium, tendon, meniscus, and bone marrow specimens obtained from 4 adult canine cadavers.<\/jats:p>\nProcedures<\/jats:bold>\u2014Synovium, tendon, meniscus, and bone marrow specimens were used to obtain synovial fibroblasts, tendon fibroblasts, meniscal fibrochondrocytes, and bone marrow\u2013derived connective tissue progenitor cells for culture on 5 biomaterials as follows: cross-linked porcine small intestine (CLPSI), non\u2013cross-linked human dermis, cross-linked porcine dermis, non\u2013cross-linked porcine small intestine (NCLPSI), and non\u2013cross-linked fetal bovine dermis. After 1 week of culture, samples were evaluated for cell viability, cell density, and extracellular matrix production. Biomaterials were evaluated in a 1-cm2<\/jats:sup> abdominal wall defect in rats. Each biomaterial was subjectively evaluated for handling, suturing, defect fit, and ease of creating the implant at the time of surgery, then grossly and histologically 6 and 12 weeks after surgery.<\/jats:p>\nResults<\/jats:bold>\u2014All biomaterials allowed for retention of viable cells in culture; however, CLPSI and NCLPSI were consistently superior in terms of cell viability and cell retention. Cell infiltration for NCLPSI was superior to other biomaterials. The NCLPSI appeared to be replaced with regenerative tissue most rapidly in vivo and scored highest in all subjective evaluations of ease of use.<\/jats:p>\nConclusions and Clinical Relevance<\/jats:bold>\u2014These data suggested that NCLPSI and CLPSI have favorable properties for further investigation of clinical application in orthopedic tissue engineering.<\/jats:p>","DOI":"10.2460\/ajvr.69.1.148","type":"journal-article","created":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T21:52:34Z","timestamp":1199224354000},"page":"148-156","source":"Crossref","is-referenced-by-count":29,"title":["In vitro and in vivo comparison of five biomaterials used for orthopedic soft tissue augmentation"],"prefix":"10.2460","volume":"69","author":[{"given":"James L.","family":"Cook","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek B.","family":"Fox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiichi","family":"Kuroki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Jayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick G.","family":"De Deyne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Am J Vet Res","first-page":"419","article-title":"Three-dimensional culture of canine articular chondrocytes on multiple transplantable substrates","volume":"58","author":"Cook","year":"1997"},{"key":"ref2","series-title":"J Knee Surg","first-page":"159","article-title":"Evaluation of small intestinal submucosa grafts for meniscal regeneration in a clinicallyrelevant posterior meniscectomy model in dogs","volume":"19","author":"Cook","year":"2006"},{"key":"ref3","series-title":"Am J Sports Med","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1177\/0363546505278702","article-title":"Long-term evaluation of treatment of large meniscal defects using small intestinal submucosa in a dog model","volume":"34","author":"Cook","year":"2006"},{"key":"ref4","series-title":"Clin Orthop Relat Res","first-page":"88","article-title":"The current status of treatment for large meniscal defects","volume":"435","author":"Cook","year":"2005"},{"key":"ref5","series-title":"Tissue Eng","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1089\/107632704322791772","article-title":"Fibrochondrogenesis of free intra-articular small intestinal submucosa scaffolds","volume":"10","author":"Fox","year":"2004"},{"key":"ref6","series-title":"Tissue Eng","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1089\/10763270152044189","article-title":"Kinetic study of the replacement of porcine small intestinal submucosa grafts and the regeneration of meniscal-like tissue in large avascular meniscal defects in dogs","volume":"7","author":"Cook","year":"2001"},{"key":"ref7","series-title":"Am J Sports Med","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1177\/03635465990270051901","article-title":"Induction of meniscal regeneration in dogs using a novel biomaterial","volume":"27","author":"Cook","year":"1999"},{"key":"ref8","series-title":"Clin Orthop","doi-asserted-by":"publisher","first-page":"S281","DOI":"10.1097\/00003086-199910001-00027","article-title":"A clinical study of collagen meniscus implants to restore the injured meniscus","volume":"367","author":"Rodkey","year":"1999"},{"key":"ref9","series-title":"J Bone Joint Surg Am","doi-asserted-by":"publisher","first-page":"1770","DOI":"10.2106\/00004623-199712000-00002","article-title":"Regeneration of meniscal cartilage with use of a collagen scaffold. 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