{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T05:23:55Z","timestamp":1741411435058,"version":"3.38.0"},"reference-count":31,"publisher":"American Veterinary Medical Association (AVMA)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To measure effects of carprofen on conductance and permeability to mannitol and histologic appearance in canine colonic mucosa.<\/jats:p>\n<jats:p><jats:bold>Sample Population<\/jats:bold>\u2014Colonic mucosa from 13 mature mixed-breed dogs.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Sections of mucosa from the transverse colon and proximal and distal portions of the descending colon were obtained immediately after dogs were euthanized. Sections were mounted in Ussing chambers. Carprofen (400 \u03bcg\/mL) was added to the bathing solution for treated sections. Conductance was calculated at 15-minute intervals for 240 minutes. Flux of mannitol was calculated for three 1-hour periods. Histologic examination of sections was performed after experiments concluded. Conductance was graphed against time for each chamber, and area under each curve was calculated. Conductance \u00d7 time, flux of mannitol, and frequency distribution of histologic findings were analyzed for an effect of region and carprofen.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Carprofen significantly increased mean conductance \u00d7 time, compared with values for control (untreated) sections for all regions of colon. Carprofen significantly increased mean flux of mannitol from period 1 to period 2 and from period 2 to period 3 for all regions of colon. Carprofen caused a significant proportion of sections to have severe sloughing of cells and erosions involving \u2265 10% of the epithelium, compared with control sections.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Carprofen increased in vitro conductance and permeability to mannitol in canine colonic mucosa. Carprofen resulted in sloughing of cells and erosion of the colonic mucosa. These findings suggested that carprofen can compromise the integrity and barrier function of the colonic mucosa of dogs.<\/jats:p>","DOI":"10.2460\/ajvr.69.2.174","type":"journal-article","created":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T22:34:31Z","timestamp":1201905271000},"page":"174-181","source":"Crossref","is-referenced-by-count":7,"title":["Effects of carprofen on the integrity and barrier function of canine colonic mucosa"],"prefix":"10.2460","volume":"69","author":[{"given":"Catherine A.","family":"Briere","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giselle","family":"Hosgood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy W.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl 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