{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T11:53:12Z","timestamp":1793274792263,"version":"4.5.0"},"reference-count":26,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To evaluate the use of technetium Tc 99m\u2013labeled EDTA-biotin monomer <jats:sup>(99m<\/jats:sup>Tc-EB1) as a scintigraphic imaging agent for soft tissue inflammatory lesions in horses.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u20146 healthy adult horses.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014First (phase 1), the agent's safety and blood-tissue clearance and an appropriate imaging protocol were determined in 6 horses. Each horse was injected with <jats:sup>99m<\/jats:sup>Tc-EB1 (1.1 GBq, IV, once); images were acquired at intervals during the following 24-hour period. Subsequently (phase 2), inflammation was induced via injection of 200 mg (10 mL) of mepivacaine (0.4 mg\/kg) into the right neck musculature and perineurally in the proximal palmar metacarpal region of the right forelimb of 2 horses. Six hours after mepivacaine injection, <jats:sup>99m<\/jats:sup>Tc-EB1 (2.2 GBq, IV, once) was administered; 8 hours after injection, comparative soft tissue images were acquired after administration of technetium <jats:sup>99m<\/jats:sup>Tc-hydroxymethylene diphosphonate (<jats:sup>99m<\/jats:sup>Tc-HDP; 7.4 GBq, IV, once).<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014After injections of <jats:sup>99m<\/jats:sup>Tc-EB1, physical examinations, CBCs, and serum biochemical analyses revealed no abnormalities in any horse. Blood clearance of <jats:sup>99m<\/jats:sup>Tc-EB1 was rapid (A phase, 2.2 minutes; \u03b2 phase, 58 minutes). Soft tissue uptake of <jats:sup>99m<\/jats:sup>Tc-EB1 was immediate and persisted for as long as 4 hours after injection. At 6 hours after IM and perineural mepivacaine injections, mepivacaine-induced inflammation was detectable by use of <jats:sub>99m<\/jats:sub>Tc-EB1.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Results indicated that <jats:sup>99m<\/jats:sup>Tc-EB1 is safe for use in horses and can identify soft tissue inflammation without concurrent uptake in bone. Compared with <jats:sup>99m<\/jats:sup>Tc-HDP administration, use of <jats:sup>99m<\/jats:sup>Tc-EB1 extended the duration of soft tissue scintigraphic image acquisition.<\/jats:p>","DOI":"10.2460\/ajvr.69.5.639","type":"journal-article","created":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T11:55:00Z","timestamp":1209642900000},"page":"639-646","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of technetium Tc 99m\u2013labeled biotin for scintigraphic detection of soft tissue inflammation in horses"],"prefix":"10.2460","volume":"69","author":[{"given":"Lauren G.","family":"Kleine","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Solano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Rusckowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen E.","family":"Hunt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen L.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl A.","family":"Kirker-Head","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Am Vet Radiol Soc","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1111\/j.1740-8261.1977.tb01127.x","article-title":"Bone and joint imaging with 99mTc\u2013labeled phosphates as a new aid in veterinary orthopedics","volume":"18","author":"Ueltschi","year":"1977"},{"key":"ref2","series-title":"J Am Vet Med Assoc","first-page":"318","article-title":"Techniques and applications of nuclear medicine in the diagnosis of equine lameness","volume":"184","author":"Devous","year":"1984"},{"key":"ref3","series-title":"J Am Vet Med Assoc","first-page":"792","article-title":"Nuclear medicine for diagnosis of lameness in horses","volume":"206","author":"Chambers","year":"1995"},{"key":"ref4","series-title":"J Am Vet Med Assoc","first-page":"390","article-title":"Scintigraphic appearance of stress-induced trauma of the dorsal cortex of the third metacarpal bone in racing Thoroughbred horses: 121 cases (1978\u20131986)","volume":"192","author":"Koblik","year":"1988"},{"key":"ref5","series-title":"Vet Radiol","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1111\/j.1740-8261.1988.tb01740.x","article-title":"Scintigraphic evaluation of skeletal disease and its application to the horse","volume":"29","author":"Lamb","year":"1988"},{"key":"ref6","series-title":"J Am Vet Med Assoc","first-page":"73","article-title":"Soft tissue- and bone-phase scintigraphy for diagnosis of navicular disease in horses","volume":"198","author":"Trout","year":"1991"},{"key":"ref7","series-title":"Vet Clin North Am Equine Pract","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S0749-0739(17)30075-5","article-title":"Equine nuclear scintigraphy. Indications, uses, and techniques","volume":"17","author":"Hoskinson","year":"2001"},{"key":"ref8","series-title":"Textbook of veterinary nuclear medicine. 2nd ed","first-page":"165","article-title":"Equine skeletal scintigraphy","author":"Graham","year":"2006"},{"key":"ref9","series-title":"Vet Clin North Am Equine Pract","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/S0749-0739(17)30498-4","article-title":"The role of scintigraphy in the lameness evaluation","volume":"7","author":"Steckel","year":"1991"},{"key":"ref10","series-title":"Textbook of veterinary nuclear medicine. 2nd ed","first-page":"363","article-title":"Scintigraphic imaging of inflammation and infection","author":"Tucker","year":"2006"},{"key":"ref11","series-title":"J Am Vet Med Assoc","first-page":"1319","article-title":"Use of 111In-labeled autologous leukocytes to image an abdominal abscess in a horse","volume":"186","author":"Koblik","year":"1985"},{"key":"ref12","series-title":"J Nucl Med","first-page":"1294","article-title":"Investigations of avidin and biotin for imaging applications","volume":"28","author":"Hnatowich","year":"1987"},{"key":"ref13","series-title":"J Nucl Med","first-page":"1810","article-title":"Localization of infection using streptavidin and biotin: an alternative to nonspecific polyclonal immunoglobulin","volume":"33","author":"Rusckowski","year":"1992"},{"key":"ref14","series-title":"Nucl Med Biol","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/0969-8051(93)90114-A","article-title":"Improved tumor localization with (strept) avidin and labeled biotin as a substitute for antibody","volume":"20","author":"Hnatowich","year":"1993"},{"key":"ref15","series-title":"Nucl Med Commun","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1097\/00006231-199501000-00008","article-title":"Influence of endogenous biotin on the biodistribution of labeled biotin derivatives in mice","volume":"16","author":"Rusckowski","year":"1995"},{"key":"ref16","series-title":"J Nucl Med","first-page":"1655","article-title":"Imaging osteomyelitis with strepavidin and indium-111-labeled biotin","volume":"37","author":"Rusckowski","year":"1996"},{"key":"ref17","series-title":"Vet Radiol","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1111\/j.1740-8261.1990.tb00795.x","article-title":"Non-skeletal distribution of bone seeking radiopharmaceuticals","volume":"31","author":"Lamb","year":"1990"},{"key":"ref18","series-title":"Equine scintigraphy","first-page":"25","article-title":"Radiopharmacy","author":"Driver","year":"2003"},{"key":"ref19","series-title":"Nuclear medicine technology and techniques. 4th ed","first-page":"160","article-title":"Radiochemistry and radiopharmacy","author":"Schwarz","year":"1997"},{"key":"ref20","series-title":"Vet Radiol","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1111\/j.1740-8261.1987.tb00049.x","article-title":"Uptake of 99mTc-MDP in muscle associated with a peripheral nerve block","volume":"28","author":"Allhands","year":"1987"},{"key":"ref21","series-title":"Exp Neurol","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/0014-4886(72)90173-2","article-title":"Some effects of local anesthetic agents on skeletal muscle","volume":"34","author":"Benoit","year":"1972"},{"key":"ref22","series-title":"Vet Radiol","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1111\/j.1740-8261.1991.tb00098.x","article-title":"The effects of regional perineural anesthesia on soft tissue and bone phase scintigraphy in the horse","volume":"32","author":"Trout","year":"1991"},{"key":"ref23","series-title":"Vet Surg","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1111\/j.1532-950X.1990.tb01154.x","article-title":"Local anesthetics and nuclear medical bone images of 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