{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T15:32:52Z","timestamp":1756308772067,"version":"3.38.0"},"reference-count":27,"publisher":"American Veterinary Medical Association (AVMA)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine the suitability and estimate the sensitivity of an immunohistochemical (IHC) test for disease-associated prion protein (PrP<jats:sup>Sc<\/jats:sup>) in biopsy specimens of rectoanal mucosa\u2013associated lymphoid tissue (RAMALT) for diagnosis of scrapie in sheep.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u2014762 sheep at high risk for having scrapie and indemnified by the National Scrapie Eradication Program.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014The IHC test for PrP<jats:sup>Sc<\/jats:sup> was applied to 2 RAMALT and 2 third-eyelid biopsy specimens and a postmortem RAMALT specimen from each sheep. Results were compared with those of a reference test in which results for tissues from obex and retropharyngeal lymph nodes, tonsil, or both were considered in parallel.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014The reference test identified 139 sheep as having scrapie. Biopsy-related complications occurred in 3 sheep. Sensitivity of the IHC test in RAMALT ranged from 85.3% to 89.4%, depending on the anatomic location from which RAMALT was obtained. Results for the test applied to 1 RAMALT specimen were similar to results interpreted in parallel for 2 third-eyelid specimens (sensitivity, 87.0%). The proportion of inconclusive test results attributable to insufficient lymphoid follicles in biopsy specimens was lower when considering results for 2 RAMALT specimens in parallel (10.1%) than when considering results for 2 third-eyelid specimens in parallel (23.7%). Specimens of RAMALT that were inappropriately collected from an area caudal to the rectoanal interface yielded a high proportion of inconclusive results (33.3% to 50.0%).<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014The IHC test for PrP<jats:sup>Sc<\/jats:sup> in RAMALT was an effective means of detecting subclinical scrapie in live, high-risk sheep.<\/jats:p>","DOI":"10.2460\/ajvr.70.1.63","type":"journal-article","created":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T21:46:38Z","timestamp":1230932798000},"page":"63-72","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation of immunohistochemical detection of prion protein in rectoanal mucosa\u2013associated lymphoid tissue for diagnosis of scrapie in sheep"],"prefix":"10.2460","volume":"70","author":[{"given":"Michelle M.","family":"Dennis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce V.","family":"Thomsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine L.","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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