{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:43:12Z","timestamp":1770219792796,"version":"3.49.0"},"reference-count":37,"publisher":"American Veterinary Medical Association (AVMA)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To assess in vivo effects of short-term administration of NSAIDs with varied cyclooxygenase (COX)-2 selectivity on pyloric and duodenal mucosa.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u20148 healthy dogs.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Each dog received deracoxib (2 mg\/kg, PO, q 24 h for 3 days), firocoxib (5 mg\/kg, PO, q 24 h for 3 days), meloxicam (0.2 mg\/kg, PO, q 24 h for 1 day followed by 0.1 mg\/kg, PO, q 24 h for 2 days), or placebo orally for 3 days; there was a 4-week interval between successive treatments. Prior to and on day 3 of drug administration, pyloric and duodenal mucosae were assessed endoscopically and biopsy specimens obtained for histologic examination. Cyclooxygenase-1 and -2 protein expressions were assessed (western blotting) and prostanoid concentrations measured (ELISAs). Data were analyzed by use of an ANOVA.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Drug administration did not significantly affect endoscopic mucosal scores, histologic scores, or COX-1 or -2 protein expression. The COX-1 protein expression was significantly higher in the pylorus than in the duodenum. Total prostaglandin and thromboxane B<jats:sub>2<\/jats:sub> (TXB<jats:sub>2<\/jats:sub>) concentrations were significantly greater in pyloric than in duodenal mucosa. Drug administration had no effect on prostaglandin or TXB<jats:sub>2<\/jats:sub> concentrations.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Prostanoid concentrations in gastric and duodenal tissues, and gross and histologic appearances, were not significantly affected by drugs with varied COX-2 selectivity. These findings suggested that, for these experimental conditions, there were no differences among the preferential and selective COX-2 inhibitors with regard to adverse effects on the gastric and duodenal portions of the gastrointestinal tract of dogs.<\/jats:p>","DOI":"10.2460\/ajvr.70.10.1243","type":"journal-article","created":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T16:04:41Z","timestamp":1254413081000},"page":"1243-1249","source":"Crossref","is-referenced-by-count":22,"title":["Effect of nonsteroidal anti-inflammatory drugs with varied cyclooxygenase-2 selectivity on cyclooxygenase protein and prostanoid concentrations in pyloric and duodenal mucosa of dogs"],"prefix":"10.2460","volume":"70","author":[{"given":"Jenna G.","family":"Wooten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony T.","family":"Blikslager","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve L.","family":"Marks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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Retrospective study of 43 cases and literature review","volume":"3","author":"Stanton","year":"1989"},{"key":"ref3","series-title":"Kirk's current veterinary therapy XII: small animal practice","first-page":"706","article-title":"Gastrointestinal ulcer therapy","author":"Matz","year":"1995"},{"key":"ref4","series-title":"N Engl J Med","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1056\/NEJM199906173402407","article-title":"Gastrointestinal toxicity of nonsteroidal antiinflammatory drugs","volume":"340","author":"Wolfe","year":"1999"},{"key":"ref5","series-title":"J Vet Intern Med","first-page":"259","article-title":"NSAID-induced gastrointestinal adverse effects in dogs\u2014can we avoid them?","volume":"17","author":"Neiger","year":"2003"},{"key":"ref6","series-title":"J Small Anim Pract","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1111\/j.1748-5827.1998.tb03748.x","article-title":"Endoscopy of the gastroduodenal mucosa after carprofen, meloxicam and ketoprofen administration in dogs","volume":"39","author":"Forsyth","year":"1998"},{"key":"ref7","series-title":"Gastroenterology","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1053\/gast.1996.v111.pm8690211","article-title":"Characterization of prostaglandin G\/H synthase 1 and 2 in rat, dog, monkey, and human gastrointestinal tracts","volume":"111","author":"Kargman","year":"1996"},{"key":"ref8","series-title":"Dig Dis Sci","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1007\/BF01297219","article-title":"Relationship of gastric mucosal damage induced in pigs by antiinflammatory drugs to their effects on prostaglandin production","volume":"27","author":"Rainsford","year":"1982"},{"key":"ref9","series-title":"JAMA","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1001\/jama.284.10.1247","article-title":"Gastrointestinal toxicity with celecoxib vs nonsteroidal anti-inflammatory drugs for osteoarthritis and rheumatoid arthritis: the CLASS study: a randomized controlled trial. Celecoxib Long-term Arthritis Safety Study","volume":"284","author":"Silverstein","year":"2000"},{"key":"ref10","series-title":"N Engl J Med","doi-asserted-by":"publisher","first-page":"1520","DOI":"10.1056\/NEJM200011233432103","article-title":"Comparison of upper gastrointestinal toxicity of rofecoxib and naproxen in patients with rheumatoid arthritis. VIGOR Study Group","volume":"343","author":"Bombardier","year":"2000"},{"key":"ref11","series-title":"FEBS Lett","first-page":"156","article-title":"Expression of mRNA for cyclooxygenase-1 and cyclooxygenase-2 in human tissues","volume":"330","author":"O'Neill","year":"1993"},{"key":"ref12","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"457","DOI":"10.2460\/ajvr.69.4.457","article-title":"Cyclooxygenase expression and prostanoid production in pyloric and duodenal mucosae in dogs after administration of nonsteroidal anti-inflammatory drugs","volume":"69","author":"Wooten","year":"2008"},{"key":"ref13","series-title":"Am J Physiol","first-page":"G1137","article-title":"Localization of cyclooxygenase-2 and regulation of its mRNA expression in gastric ulcers in rats","volume":"275","author":"Takahashi","year":"1998"},{"key":"ref14","series-title":"Gastroenterology","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1053\/gast.1997.v112.pm9024292","article-title":"Induction of cyclooxygenase 2 in gastric mucosal lesions and its inhibition by the specific antagonist delays healing in mice","volume":"112","author":"Mizuno","year":"1997"},{"issue":"suppl 5","key":"ref15","series-title":"J Physiol Pharmacol","first-page":"33","article-title":"Role of prostaglandins in gastroprotection and gastric adaptation","volume":"56","author":"Brzozowski","year":"2005"},{"key":"ref16","series-title":"Gut","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1136\/gut.49.3.443","article-title":"Cyclooxygenase 2-implications on maintenance of gastric mucosal integrity and ulcer healing: controversial issues and perspectives","volume":"49","author":"Halter","year":"2001"},{"key":"ref17","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"76","DOI":"10.2460\/ajvr.68.1.76","article-title":"Expression of cyclooxygenase1 and -2 in naturally occurring squamous cell carcinomas in horses","volume":"68","author":"Elce","year":"2007"},{"key":"ref18","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"802","DOI":"10.2460\/ajvr.2000.61.802","article-title":"In vitro effects of nonsteroidal anti-inflammatory drugs on cyclooxygenase activity in dogs","volume":"61","author":"Kay-Mugford","year":"2000"},{"key":"ref19","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"91","DOI":"10.2460\/AJVR.2002.63.91","article-title":"Cyclooxygenase selectivity of nonsteroidal anti-inflammatory drugs in canine blood","volume":"63","author":"Streppa","year":"2002"},{"key":"ref20","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"503","DOI":"10.2460\/ajvr.2004.65.503","article-title":"In vitro effects and in vivo efficacy of a novel cyclooxygenase-2 inhibitor in dogs with experimentally induced synovitis","volume":"65","author":"McCann","year":"2004"},{"key":"ref21","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1016\/j.cvsm.2006.07.001","article-title":"The clinical pharmacology of cyclooxygenase-2-selective and dual inhibitors","volume":"36","author":"Clark","year":"2006"},{"key":"ref22","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"812","DOI":"10.2460\/ajvr.2005.66.812","article-title":"In vivo effects of carprofen, deracoxib, and etodolac on prostanoid production in blood, gastric mucosa, and synovial fluid in dogs with chronic osteoarthritis","volume":"66","author":"Sessions","year":"2005"},{"key":"ref23","series-title":"Vet Ther","first-page":"41","article-title":"Firocoxib efficacy preventing urate-induced synovitis, pain, and inflammation in dogs","volume":"8","author":"Drag","year":"2007"},{"key":"ref24","series-title":"J Vet Emerg Crit Care","first-page":"34","article-title":"Gastrointestinal perforation in five dogs associated with the administration of meloxicam","volume":"16","author":"Enberg","year":"2006"},{"key":"ref25","series-title":"Compend Contin Educ Pract Vet","first-page":"688","article-title":"Helicobacter infection in humans and animals","volume":"24","author":"Flatland","year":"2002"},{"key":"ref26","series-title":"J Clin Microbiol","doi-asserted-by":"publisher","first-page":"3165","DOI":"10.1128\/jcm.34.12.3165-3170.1996","article-title":"Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: animal and public health implications","volume":"34","author":"Eaton","year":"1996"},{"key":"ref27","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"810","DOI":"10.2460\/ajvr.2004.65.810","article-title":"Determination of expression of cyclooxygenase-1 and -2 isozymes in canine tissues and their differential sensitivity to nonsteroidal anti-inflammatory drugs","volume":"65","author":"Wilson","year":"2004"},{"key":"ref28","series-title":"Nat Med","doi-asserted-by":"publisher","first-page":"1418","DOI":"10.1038\/70995","article-title":"Inhibition of angiogenesis by nonsteroidal anti-inflammatory drugs: insight into mechanisms and implications for cancer growth and ulcer healing","volume":"5","author":"Jones","year":"1999"},{"key":"ref29","series-title":"Proc Natl Acad Sci U S A","doi-asserted-by":"publisher","first-page":"13243","DOI":"10.1073\/pnas.202392199","article-title":"Divergent effects of new cyclooxygenase inhibitors on gastric ulcer healing: shifting the angiogenic balance","volume":"99","author":"Ma","year":"2002"},{"key":"ref30","series-title":"Am J Physiol Gastrointest Liver Physiol","doi-asserted-by":"publisher","first-page":"G747","DOI":"10.1152\/ajpgi.00416.2005","article-title":"Role of the different isoforms of cyclooxygenase and nitric oxide synthase during gastric ulcer healing in cyclooxygenase-1 and -2 knockout mice","volume":"290","author":"Schmassmann","year":"2006"},{"key":"ref31","series-title":"Cell","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/0092-8674(95)90126-4","article-title":"Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration","volume":"83","author":"Langenbach","year":"1995"},{"key":"ref32","series-title":"Aliment Pharmacol Ther","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1046\/j.1365-2036.1997.00247.x","article-title":"Aspirin causes rapid up-regulation of cyclo-oxygenase-2 expression in the stomach of rats","volume":"11","author":"Davies","year":"1997"},{"key":"ref33","series-title":"Gastroenterology","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/S0016-5085(98)70370-1","article-title":"Cyclooxygenase 1 contributes to inflammatory responses in rats and mice: implications for gastrointestinal toxicity","volume":"115","author":"Wallace","year":"1998"},{"key":"ref34","series-title":"Nat Med","doi-asserted-by":"publisher","first-page":"698","DOI":"10.1038\/9550","article-title":"Inducible cyclooxygenase may have anti-inflammatory properties","volume":"5","author":"Gilroy","year":"1999"},{"key":"ref35","series-title":"Eur J Pharmacol","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/S0014-2999(98)00508-1","article-title":"Differential effects of inhibitors of cyclooxygenase (cyclooxygenase 1 and cyclooxygenase 2) in acute inflammation","volume":"355","author":"Gilroy","year":"1998"},{"key":"ref36","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"251","DOI":"10.2460\/ajvr.68.3.251","article-title":"Changes in platelet function, hemostasis, and prostaglandin expression after treatment with nonsteroidal anti-inflammatory drugs with various cyclooxygenase selectivities in 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