{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T22:51:24Z","timestamp":1780354284995,"version":"3.54.1"},"reference-count":33,"publisher":"American Veterinary Medical Association (AVMA)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine the effect of feline herpesvirus type 1 (FHV-1) on tear film breakup time (TFBUT) and Schirmer tear test (STT) values in cats with primary experimental infection and to determine the relationship betweenTFBUT and STT values and conjunctival goblet cell density (GCD).<\/jats:p>\n<jats:p><jats:bold>Sample Population<\/jats:bold>\u20149 specific-pathogen\u2013free cats of approximately 6 months of age.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u20146 cats were inoculated with FHV-1; 3 control cats were sham inoculated. Clinical and histologic evidence of conjunctivitis and TFBUT, GCD, and STT values were assessed at multiple times until postinoculation day (PID) 29.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014In infected cats, mean clinical and histologic conjunctivitis scores peaked at PID 7 and remained above baseline at PID 29. In control cats, these 2 variables did not change from baseline throughout the study. MeanTFBUT declined rapidly in infected cats up to PID 15 and at PID 29 remained less than baseline, less than for control cats, and below refer-ence range values. Mean STT value for infected cats at PID 29 was increased from baseline but was within the reference range and not different from the value for control cats. Mean GCD in infected cats declined precipitously by PID 7 and remained below reference range values at PID 29. Mean GCD in control cats remained unchanged for the duration of the study period.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014FHV-1 induced qualitative tear film abnormalities in experimentally infected cats, as measured by TFBUT and GCD. Assessment of TFBUT provided a reasonable clinical estimate of GCD.<\/jats:p>","DOI":"10.2460\/ajvr.70.3.394","type":"journal-article","created":{"date-parts":[[2009,3,2]],"date-time":"2009-03-02T13:54:42Z","timestamp":1236002082000},"page":"394-403","source":"Crossref","is-referenced-by-count":49,"title":["Effects of feline herpesvirus type 1 on tear film break-up time, Schirmer tear test results, and conjunctival goblet cell density in experimentally infected cats"],"prefix":"10.2460","volume":"70","author":[{"given":"Christine C.","family":"Lim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Reilly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara M.","family":"Thomasy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip 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anesthesia","volume":"8","author":"Cullen","year":"2005"},{"key":"ref4","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1016\/S0195-5616(90)50056-X","article-title":"Feline herpesvirus ocular disease","volume":"20","author":"Nasisse","year":"1990"},{"key":"ref5","series-title":"Aust Vet J","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1111\/j.1751-0813.1981.tb00471.x","article-title":"Feline respiratory virus carriers in clinically healthy cats","volume":"57","author":"Ellis","year":"1981"},{"key":"ref6","series-title":"Surv Ophthalmol","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/0039-6257(76)90102-8","article-title":"Feline herpes virus infection","volume":"21","author":"Bodle","year":"1976"},{"key":"ref7","series-title":"Invest Ophthalmol Vis Sci","first-page":"1758","article-title":"Experimental ocular herpesvirus infection in the cat","volume":"30","author":"Nasisse","year":"1989"},{"key":"ref8","series-title":"Am J Vet Res","first-page":"51","article-title":"Immunologic, histologic, and virologic features of herpesvirus-induced stromal keratitis in cats","volume":"56","author":"Nasisse","year":"1995"},{"key":"ref9","series-title":"J Am Vet Med Assoc","first-page":"834","article-title":"Clinical and laboratory findings in chronic conjunctivitis in cats: 91 cases (1983\u20131991)","volume":"203","author":"Nasisse","year":"1993"},{"key":"ref10","series-title":"Vet Ophthalmol","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1111\/j.1463-5224.2005.00403.x","article-title":"Schirmer tear test values and tear film break-up times in cats with conjunctivitis","volume":"8","author":"Lim","year":"2005"},{"key":"ref11","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"37","DOI":"10.2460\/ajvr.2003.64.37","article-title":"Efficacy of oral supplementation with L-lysine in cats latently infected with feline herpesvirus","volume":"64","author":"Maggs","year":"2003"},{"key":"ref12","series-title":"Graefes Arch Clin Exp Ophthalmol","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1007\/s00417-002-0429-3","article-title":"Morphologic evidence of M cells in healthy canine conjunctiva-associated lymphoid tissue","volume":"240","author":"Giuliano","year":"2002"},{"key":"ref13","series-title":"Mod Vet Pract","first-page":"48","article-title":"The Schirmer tear test\u2014in cats","volume":"51","author":"Veith","year":"1970"},{"key":"ref14","series-title":"Vet Ophthalmol","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1111\/j.1463-5224.2005.00362.x","article-title":"Qualitative tear film and conjunctival goblet cell assessment of cats with corneal sequestra","volume":"8","author":"Grahn","year":"2005"},{"key":"ref15","series-title":"Surv Ophthalmol","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/0039-6257(77)90087-X","article-title":"Tear physiology and dry eyes","volume":"22","author":"Holly","year":"1977"},{"key":"ref16","series-title":"Prog Retin Eye Res","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1016\/S1350-9462(96)00039-0","article-title":"Ocular mucins: purification, metabolism and functions","volume":"16","author":"Corfield","year":"1997"},{"key":"ref17","series-title":"Cornea","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1097\/01.ico.0000154378.67495.40","article-title":"The effect of instilled fluorescein solution volume on the values and repeatability of TBUT measurements","volume":"24","author":"Johnson","year":"2005"},{"key":"ref18","series-title":"Am J Vet Res","first-page":"820","article-title":"Effects of inflammation and aqueous tear film deficiency on conjunctival morphology and ocular mucus composition in cats","volume":"51","author":"Johnson","year":"1990"},{"key":"ref19","series-title":"Vet Ophthalmol","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1046\/j.1463-5224.1999.00082.x","article-title":"Ulcerative keratitis associated with qualitative tear film abnormalities in cats","volume":"2","author":"Cullen","year":"1999"},{"key":"ref20","series-title":"Exp Eye Res","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S0014-4835(61)80006-7","article-title":"The oily layer of the tear film and evaporation from the corneal surface","volume":"1","author":"Mishima","year":"1961"},{"key":"ref21","series-title":"J Am Vet Med Assoc","first-page":"511","article-title":"Inconsistencies in the absorptive capacities of Schirmer tear test strips","volume":"188","author":"Hawkins","year":"1986"},{"key":"ref22","series-title":"J Am Vet Med Assoc","first-page":"1286","article-title":"Effect of topical pilocarpine treatment on tear production in dogs","volume":"205","author":"Smith","year":"1994"},{"key":"ref23","series-title":"Eur J Pharmacol","first-page":"327","article-title":"In vivo effects of indomethacin and flurbiprofen on the locomotion of neutrophils elicited by immune and non-immune inflammation in the rat","volume":"106","author":"Perianin","year":"1985"},{"key":"ref24","series-title":"Invest Ophthalmol Vis Sci","first-page":"653","article-title":"Effect of cyclosporine on conjunctival mucin in a canine keratoconjunctivitis sicca model","volume":"42","author":"Moore","year":"2001"},{"key":"ref25","series-title":"Exp Eye Res","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S0014-4835(87)80151-3","article-title":"Effects of cyclosporine A on the corneal inflammatory response in herpes simplex virus keratitis","volume":"45","author":"Meyers-Elliott","year":"1987"},{"key":"ref26","series-title":"Arch Ophthalmol","doi-asserted-by":"publisher","first-page":"1804","DOI":"10.1001\/archopht.1984.01040031462023","article-title":"Prophylactic topical cyclosporine in experimental herpetic stromal keratitis","volume":"102","author":"Boisjoly","year":"1984"},{"key":"ref27","series-title":"Am J Ophthalmol","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1016\/j.ajo.2005.11.042","article-title":"Treatment of herpes simplex virus stromal keratitis unresponsive to topical prednisolone 1% with topical cyclosporine 0.05%","volume":"141","author":"Rao","year":"2006"},{"key":"ref28","series-title":"Cornea","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1097\/00003226-199207000-00003","article-title":"Ocular surface residence times of artificial tear solutions","volume":"11","author":"Snibson","year":"1992"},{"key":"ref29","series-title":"Jpn J Ophthalmol","first-page":"62","article-title":"Sodium hyaluronate eyedrops enhance tear film stability","volume":"40","author":"Hamano","year":"1996"},{"key":"ref30","series-title":"Br J Ophthalmol","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1136\/bjo.70.6.442","article-title":"Effect of sodium hyaluronate (0.1%) on break-up time (NIBUT) in patients with dry eyes","volume":"70","author":"Mengher","year":"1986"},{"key":"ref31","series-title":"Graefes Arch Clin Exp Ophthalmol","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1007\/s00417-005-0028-1","article-title":"Effectiveness of sodium hyaluronate eyedrops in the treatment of dry eye","volume":"244","author":"Johnson","year":"2006"},{"key":"ref32","series-title":"Invest Ophthalmol Vis Sci","first-page":"308","article-title":"Preservative cytotoxic threshold for benzalkonium chloride and chlorhexidine digluconate in cat and rabbit corneas","volume":"19","author":"Burstein","year":"1980"},{"key":"ref33","series-title":"Mol Vis","first-page":"415","article-title":"Impact of short-term exposure of commercial eyedrops preserved with benzalkonium chloride on precorneal mucin","volume":"12","author":"Chung","year":"2006"}],"container-title":["American Journal of Veterinary 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