{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T03:04:04Z","timestamp":1770433444967,"version":"3.49.0"},"reference-count":36,"publisher":"American Veterinary Medical Association (AVMA)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To characterize the expression of the cyclooxygenase (COX)-1 and COX-2 isoforms in naturally occurring ulcers of the nonglandular portion of the stomach in horses.<\/jats:p>\n<jats:p><jats:bold>Specimen Population<\/jats:bold>\u201438 specimens from ulcerated stomachs and 10 specimens from healthy stomachs.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Specimens were collected at an abbatoir; for each specimen of squamous gastric mucosa, 1 portion was fixed in neutral-buffered 10% formalin for immunohistochemical analysis and another was frozen at \u221270\u00b0C for immunoblotting analysis. Immunoreactivity to 2 antibodies, MF241 (selective for COX-1) and MF243 (selective for COX-2), was evaluated by a veterinary pathologist using a scoring system. Expression of COX-1 and COX-2 was confirmed by use of immunoblotting analyses.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014All specimens from healthy stomachs strongly expressed COX-1, whereas only 2 of 10 expressed COX-2. The expression of both isoforms varied greatly in the ulcerated mucosal specimens. Expression of COX-1 was significantly lower and expression of COX-2 was significantly higher in ulcerated versus healthy specimens.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Increased expression of COX-2 in gastric ulcers of the squamous portion of the stomach in horses suggested a role for this enzyme in gastric ulcer healing.<\/jats:p>","DOI":"10.2460\/ajvr.71.5.592","type":"journal-article","created":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T07:05:35Z","timestamp":1272697535000},"page":"592-596","source":"Crossref","is-referenced-by-count":8,"title":["Expression of cyclooxygenase isoforms in ulcerated tissues of the nonglandular portion of the stomach in horses"],"prefix":"10.2460","volume":"71","author":[{"given":"Nat\u00e1lia L. F.","family":"Rodrigues","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique","family":"Dor\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mich\u00e8le Y.","family":"Doucet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"Proceedings","first-page":"256","article-title":"Prevalence of gastric ulcers in elite, heavy use western performance horses","author":"Bertone","year":"2000"},{"key":"ref2","series-title":"J Vet Intern Med","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1111\/j.1939-1676.2003.tb02437.x","article-title":"Gastric ulcers in standardbred racehorses: prevalence, lesion description, and risk factors","volume":"17","author":"Dionne","year":"2003"},{"issue":"suppl 1","key":"ref3","series-title":"Vet Res 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