{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T05:22:48Z","timestamp":1741411368308,"version":"3.38.0"},"reference-count":26,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To assess viability of innervation in bowel segments appearing macroscopically viable from dogs with intussusception.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u20147 dogs without gastrointestinal dysfunction that had been euthanized for reasons unrelated to the study (control dogs) and 13 dogs with intussusception that underwent enterectomy and intestinal anastomosis (affected dogs).<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014A total of 31 samples of intestinal tissue were obtained from the control dogs; 28 samples were obtained from affected dogs during surgery. Samples were histologically and immunohistochemically prepared and subjectively scored for degree of vacuolization and staining, respectively. Other data collected included mean muscle cell density of circular and longitudinal muscular layers, ratio between areas of muscular layers, mean number of myenteric plexuses, mean ganglion cell density of myenteric plexuses, and degree of degeneration in neuronal plexuses as estimated through synaptophysin and neuron-specific enolase (NSE) immunoreactivity.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Mean muscle cell density of longitudinal muscular layers, ratio between areas of muscular layers, and synaptophysin immunoreactivity did not differ significantly between affected and control dogs; values of all other variables did. Correlations were evident between mean ganglion cell density in myenteric plexuses and mean muscle cell density in circular muscular layers, degree of neuronal degeneration in myenteric plexuses and NSE immunoreactivity, and degree of neuronal degeneration in myenteric plexuses and mean ganglion cell density of myenteric plexuses.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Innervation may be impaired in bowel segments that appear macroscopically viable. Therefore, careful evaluation of preserved surgical margins during enterectomy and enteroanastomosis and monitoring of digestive function after surgery are important.<\/jats:p>","DOI":"10.2460\/ajvr.71.6.636","type":"journal-article","created":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T07:06:16Z","timestamp":1275375976000},"page":"636-642","source":"Crossref","is-referenced-by-count":1,"title":["Histologic and immunohistochemical evaluation of intestinal innervation in dogs with and without intussusception"],"prefix":"10.2460","volume":"71","author":[{"given":"Leda M.","family":"Oliveira-Barros","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais A.","family":"Costa-Casagrande","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Cogliati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00edlian R. 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Fetal intestinal obstruction induces alteration of enteric nervous system development in human intestinal atresia","volume":"56","author":"Khen","year":"2004"},{"key":"ref7","series-title":"J Surg Res","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1006\/jsre.1993.1188","article-title":"Determination of small bowel viability using quantitative myoelectric and color analysis","volume":"55","author":"Orland","year":"1993"},{"key":"ref8","series-title":"Tratado de clinica cir\u00fargica do sistema digest\u00f3rio\u2014intestino delgado. S\u00e3o","first-page":"1131","article-title":"Aguda","author":"Rocha","year":"2004"},{"key":"ref9","series-title":"J Am Vet Med Assoc","first-page":"928","article-title":"Morphologic and quantitative evaluation of the myenteric plexuses and neurons in the large colon of horses","volume":"210","author":"Schusser","year":"1997"},{"key":"ref10","series-title":"Pediatr Surg Int","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1007\/s003830050376","article-title":"Qualitative and quantitative analysis of synapses in Hirschsprung's disease","volume":"13","author":"Wiedenmann","year":"1998"},{"key":"ref11","series-title":"Pediatr Dev Pathol","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s100240010115","article-title":"Morphometric assessment of Hirschsprung's disease: associated hypoganglionosis of the colonic myenteric plexus","volume":"4","author":"Meier-Ruge","year":"2001"},{"key":"ref12","series-title":"J Physiol","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1111\/j.1469-7793.2001.0555c.xd","article-title":"Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction","volume":"536","author":"Chang","year":"2001"},{"key":"ref13","series-title":"Neurogastroenterol Motil","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1046\/j.1365-2982.2002.00317.x","article-title":"Immunoreactivity of Hu proteins facilitates identification of myenteric neurones in guinea-pig small intestine","volume":"14","author":"Lin","year":"2002"},{"key":"ref14","series-title":"J Gastrointest Surg","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1016\/S1091-255X(02)00154-3","article-title":"Neuronal adrenergic and muscular cholinergic contractile hypersensitivity in canine jejunum after extrinsic denervation","volume":"7","author":"Balsiger","year":"2003"},{"key":"ref15","series-title":"Equine Vet J","first-page":"474","article-title":"Interstitial cells of Cajal (ICC) in equine colic: an immunohistochemical study of horses with obstructive disorders of the small and large intestines","volume":"36","author":"Fintl","year":"2004"},{"key":"ref16","series-title":"Neuropathology","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1111\/j.1440-1789.2006.00649.x","article-title":"Histopathological and immunohistochemical study of the enteric innervations among various types of aganglionoses including isolated and syndromic Hirschsprung disease","volume":"26","author":"Matsuda","year":"2006"},{"key":"ref17","series-title":"World J Gastroenterol","doi-asserted-by":"publisher","first-page":"192","DOI":"10.3748\/wjg.15.192","article-title":"New techniques in the tissue diagnosis of gastrointestinal neuromuscular diseases","volume":"15","author":"Knowles","year":"2009"},{"key":"ref18","series-title":"Cirurgia de pequenos animais","first-page":"322","article-title":"Cirurgia do intestino delgado","author":"Hedlung","year":"2002"},{"key":"ref19","series-title":"Clin Tech Small Anim Pract","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1053\/svms.2002.36606","article-title":"Gastrointestinal obstruction","volume":"17","author":"Macphail","year":"2002"},{"key":"ref20","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.2460\/javma.2001.219.1415","article-title":"Complications of enteroplication for the prevention of intussusception recurrence in dogs: 35 cases (1989\u20131999)","volume":"219","author":"Applewhite","year":"2001"},{"key":"ref21","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1016\/S0195-5616(00)50039-4","article-title":"Gastrointestinal emergencies","volume":"30","author":"Aronson","year":"2000"},{"key":"ref22","series-title":"World J Surg","doi-asserted-by":"publisher","first-page":"1512","DOI":"10.1007\/s00268-007-9070-y","article-title":"Reduced neuronal innervation in the distal end of the proximal esophageal atretic segment in cases of esophageal atresia with distal tracheoesophageal fistula","volume":"31","author":"Boleken","year":"2007"},{"key":"ref23","series-title":"J Pediatr Gastroenterol Nutr","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1097\/01.mpg.0000218696.58487.5b","article-title":"Enteric nervous system disease and recovery, plasticity and regeneration","volume":"42","author":"Belkind-Gerson","year":"2006"},{"key":"ref24","series-title":"J Pediatr Surg","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1053\/jpsu.2002.32267","article-title":"Experimental small bowel obstruction in chick embryos: effects on the developing enteric nervous system","volume":"37","author":"Schoenberg","year":"2002"},{"key":"ref25","series-title":"J Histochem Cytochem","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1177\/002215549904701107","article-title":"Quantitative comparison of growth-associated protein GAP-43, Neuron-specific Enolase, and Protein Gene Product 9.5 as neuronal markers in mature human intestine","volume":"47","author":"Vento","year":"1999"},{"key":"ref26","series-title":"J Vet Med A Physiol Pathol Clin Med","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1111\/j.1439-0442.2004.00681.x","article-title":"Caecal impaction and chronic intestinal pseudo-obstruction in a dog","volume":"52","author":"Eastwood","year":"2005"}],"container-title":["American Journal of Veterinary 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