{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T22:36:02Z","timestamp":1787697362577,"version":"build-2784847793"},"reference-count":23,"publisher":"American Veterinary Medical Association (AVMA)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine erythrocyte survival time in Greyhounds.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u20146 Greyhounds used as blood donors and 3 privately owned non-Greyhound dogs.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014In vivo biotinylation of erythrocytes was performed by infusion of biotin\u2014Nhydroxysuccinimide into each dog via a jugular vein catheter. Blood samples were collected 12 hours later and then at weekly intervals and were used to determine the percentage of biotin-labeled erythrocytes at each time point. Erythrocytes were washed, incubated with avidin\u2014fluorescein isothiocyanate, and washed again before the percentage of biotinylated erythrocytes was measured by use of flow cytometry. Survival curves for the percentage of biotinylated erythrocytes were generated, and erythrocyte survival time was defined as the x-intercept of a least squares best-fit line for the linear portion of each curve.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014The R<jats:sup>2<\/jats:sup> for survival curves ranged from 0.93 to 0.99 during the first 10 weeks after infusion of erythrocytes. Erythrocyte survival time for the 3 non-Greyhound dogs was 94, 98, and 116 days, respectively, which was consistent with previously reported values. Erythrocyte survival time for the 6 Greyhounds ranged from 83 to 110 days (mean, 93 days; median, 88 days). As determined by use of in vivo biotinylation, erythrocyte survival times in Greyhounds were similar to those determined for non-Greyhound dogs and did not differ significantly from erythrocyte survival times reported previously for non-Greyhound dogs.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Erythrocyte survival time was similar in Greyhounds and non-Greyhound dogs. Greyhounds can be used as erythrocyte donors without concerns about inherently shorter erythrocyte survival time. <jats:italic>(Am J Vet Res<\/jats:italic> 2010;71:1033\u20131038)<\/jats:p>","DOI":"10.2460\/ajvr.71.9.1033","type":"journal-article","created":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T07:12:57Z","timestamp":1283325177000},"page":"1033-1038","source":"Crossref","is-referenced-by-count":7,"title":["Erythrocyte survival time in Greyhounds as assessed by use of in vivo biotinylation"],"prefix":"10.2460","volume":"71","author":[{"given":"Catherine L.","family":"Garon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah A.","family":"Cohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1272","reference":[{"key":"ref1","series-title":"J Am Anim Hosp Assoc","doi-asserted-by":"publisher","first-page":"303","DOI":"10.5326\/15473317-32-4-303","article-title":"Canine red blood cell transfusion practice","volume":"32","author":"Callan","year":"1996"},{"key":"ref2","series-title":"J Am Vet Med Assoc","first-page":"1495","article-title":"Packed red cell transfusions in dogs: 131 cases (1989)","volume":"202","author":"Kerl","year":"1993"},{"key":"ref3","series-title":"J Am Vet Med Assoc","first-page":"402","article-title":"Greyhound blood donors","volume":"205","author":"Dodds","year":"1994"},{"key":"ref4","series-title":"J Am Vet Med Assoc","first-page":"1295","article-title":"Banking on Greyhounds","volume":"204","author":"Kahler","year":"1994"},{"key":"ref5","series-title":"J Am Vet Med Assoc","first-page":"1689","article-title":"Veterinarians probe Greyhound idiosyncrasies","volume":"206","author":"Smith","year":"1995"},{"key":"ref6","series-title":"J Am Vet Med Assoc","first-page":"1603","article-title":"Hematologic values in mongrel and Greyhound dogs being screened for research use","volume":"159","author":"Porter","year":"1971"},{"key":"ref7","series-title":"J Am Vet Med Assoc","first-page":"838","article-title":"Platelet concentration and hemoglobin function in Greyhounds","volume":"205","author":"Sullivan","year":"1994"},{"key":"ref8","series-title":"Am J Vet Res","first-page":"739","article-title":"Determination of the lifespan of erythrocytes from Greyhounds, using an in vitro biotinylation technique","volume":"57","author":"Novinger","year":"1996"},{"key":"ref9","series-title":"Blood","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1182\/blood.V93.1.376","article-title":"Evaluation of biochemical changes during in vivo erythrocyte senescence in the dog","volume":"93","author":"Rettig","year":"1999"},{"key":"ref10","series-title":"Cell Physiol Biochem","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1159\/000089839","article-title":"Band 3\/complement-mediated recognition and removal of normally senescent and pathological human erythrocytes","volume":"16","author":"Arese","year":"2005"},{"key":"ref11","series-title":"Blood","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.1182\/blood.V8.12.1110.1110","article-title":"Red blood cell survival studies","volume":"8","author":"Eadie","year":"1953"},{"key":"ref12","series-title":"Red blood cells of domestic mammals","first-page":"67","article-title":"Red cell life span","author":"Vacha","year":"1983"},{"key":"ref13","series-title":"Blood","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1182\/blood.V19.6.715.715","article-title":"Red blood cell life span using DFP as a cohort label","volume":"19","author":"Cline","year":"1962"},{"key":"ref14","series-title":"Nucl Med Biol","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1016\/0969-8051(93)90146-L","article-title":"Radioisotopic labels for blood cell survival studies: a review","volume":"20","author":"de Vries","year":"1993"},{"key":"ref15","series-title":"Blood","doi-asserted-by":"publisher","first-page":"2172","DOI":"10.1182\/blood.V74.6.2172.2172","article-title":"Fluorescent in vivo tracking of hematopoietic cells. 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