{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:22:05Z","timestamp":1770596525810,"version":"3.49.0"},"reference-count":44,"publisher":"American Veterinary Medical Association (AVMA)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To characterize amphotericin B\u2013induced lameness in cattle and to ascertain the analgesic effects of flunixin meglumine by use of multimodal assessment.<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u201410 healthy Holstein steers free from musculoskeletal disease.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Steers were randomly allocated to a treatment or negative control group. Amphotericin B was injected into the distal interphalangeal joint of the lateral claw of the left hind limb of all steers. Treatment steers received flunixin meglumine at the time of synovitis-arthritis induction and at 12 hours after induction. Control steers received no medication. Multimodal analysis included vital parameters, visual lameness score, behavioral monitoring with accelerometers, pressure mat analysis, and plasma cortisol determination before and after induction. Data were analyzed by use of linear mixed models with treatment and time designated as fixed effects, accounting for repeated measures on individual calves.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Amphotericin B injection induced moderate, transient lameness. Control steers were more than twice as likely to be lame as treatment steers (mean \u00b1 SD lameness score, 92.2 \u00b1 8.1 % vs 40.7 \u00b1 2.5%). Treatment steers placed significantly greater force and contact area on the affected foot and greater force, impulse, and contact area on the paired claw, compared with control steers. Furthermore, treatment steers spent considerably less time in recumbency than controls.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Amphotericin B successfully induced synovitis-arthritis in dairy steers that was transient in nature. Flunixin meglumine was efficacious in providing analgesia for these steers.<\/jats:p>","DOI":"10.2460\/ajvr.72.11.1431","type":"journal-article","created":{"date-parts":[[2011,10,24]],"date-time":"2011-10-24T18:06:08Z","timestamp":1319479568000},"page":"1431-1438","source":"Crossref","is-referenced-by-count":48,"title":["Effect of flunixin meglumine on the amelioration of lameness in dairy steers with amphotericin B-induced transient synovitis-arthritis"],"prefix":"10.2460","volume":"72","author":[{"given":"Kara L.","family":"Schulz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johann F.","family":"Coetzee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brad 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example","volume":"91","author":"Bicalho","year":"2008"},{"key":"ref7","series-title":"J Dairy Sci","doi-asserted-by":"publisher","first-page":"4586","DOI":"10.3168\/jds.2007-0297","article-title":"Visual locomotion scoring in the first seventy days in milk: impact on pregnancy and survival","volume":"90","author":"Bicalho","year":"2007"},{"key":"ref8","series-title":"J Dairy Sci","doi-asserted-by":"publisher","first-page":"4115","DOI":"10.3168\/jds.S0022-0302(04)73554-7","article-title":"Effect of lameness on culling in dairy cows","volume":"87","author":"Booth","year":"2004"},{"key":"ref9","series-title":"J Dairy Sci","doi-asserted-by":"publisher","first-page":"1969","DOI":"10.3168\/jds.S0022-0302(01)74639-5","article-title":"A longitudinal field trial of the effect of biotin on lameness in dairy cows","volume":"84","author":"Hedges","year":"2001"},{"key":"ref10","series-title":"Vet Rec","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1136\/vr.153.7.197","article-title":"Assessment of the welfare of dairy cattle using animal-based measurements: direct observations and investigation of farm records","volume":"153","author":"Whay","year":"2003"},{"key":"ref11","series-title":"Cattle Pract","first-page":"69","article-title":"Non-steroidal anti-inflammatory drugs in cattle\u2014should we use them more?","volume":"12","author":"Barrett","year":"2004"},{"key":"ref12","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"528","DOI":"10.2460\/javma.235.5.528","article-title":"Update on drugs prohibited from extralabel use in food animals","volume":"235","author":"Davis","year":"2009"},{"key":"ref13","series-title":"Vet Rec","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1136\/vr.157.23.729","article-title":"Role of ketoprofen in the modulation of hyperalgesia associated with lameness in dairy cattle","volume":"157","author":"Whay","year":"2005"},{"key":"ref14","series-title":"Cattle behavior and welfare","author":"Phillips","year":"2002"},{"key":"ref15","series-title":"Anim Welf","first-page":"31","article-title":"Assessing pain in animals","volume":"11","author":"Rutherford","year":"2002"},{"key":"ref16","series-title":"Vet J","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/S1090-0233(98)80058-0","article-title":"The influence of lesion type on the duration of hyperalgesia associated with hind limb lameness in dairy cattle","volume":"156","author":"Whay","year":"1998"},{"key":"ref17","series-title":"J Vet Med A Physiol Pathol Clin Med","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1111\/j.1439-0442.2006.00837.x","article-title":"Changes in MMP-2 and -9 activity and MMP-8 reactivity after amphotericin B induced synovitis and treatment with bufexamac","volume":"53","author":"Marttinen","year":"2006"},{"key":"ref18","series-title":"Am J Vet Res","first-page":"1467","article-title":"Effects of intra-articular injections of bufexamac suspension on amphotericin B\u2013induced aseptic arthritis in horses","volume":"60","author":"Suominen","year":"1999"},{"key":"ref19","series-title":"Am J Vet Res","first-page":"1527","article-title":"Effects of hylan on amphotericin-induced carpal lameness in equids","volume":"54","author":"Peloso","year":"1993"},{"key":"ref20","series-title":"Am J Vet Res","first-page":"1535","article-title":"Computer-assisted three-dimensional gait analysis of amphotericin-induced carpal lameness in horses","volume":"54","author":"Peloso","year":"1993"},{"key":"ref21","series-title":"Can J Vet Res","first-page":"76","article-title":"Pulsed radio frequency therapy of experimentally induced arthritis in ponies","volume":"55","author":"Crawford","year":"1991"},{"key":"ref22","series-title":"Am J Vet Res","first-page":"51","article-title":"Thermographic evaluation of corticosteroid efficacy in amphotericin B-induced arthritis in ponies","volume":"44","author":"Bowman","year":"1983"},{"key":"ref23","series-title":"Vet Surg","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1111\/j.1532-950X.1996.tb01452.x","article-title":"Efficacy of an epidural combination of morphine and detomidine in alleviating experimental induced hind limb lameness in horses","volume":"25","author":"Sysel","year":"1996"},{"key":"ref24","series-title":"Vet Med J Giza","first-page":"305","article-title":"Clinical, biochemical and histopathological studies on arthritis in equines","volume":"42","author":"Fahmy","year":"1994"},{"key":"ref25","series-title":"Vet Med J Giza","first-page":"175","article-title":"Comparison of corticosteroids and dimethylsulfoxide treatment in chemically induced arthritis in horses","volume":"42","author":"Fahmy","year":"1994"},{"key":"ref26","series-title":"Vet Med J Giza","first-page":"221","article-title":"Evaluation of the effects of intra-articular injection of dimethylsulfoxide on chemically induced arthritis in equines","volume":"42","author":"Hegazy","year":"1994"},{"key":"ref27","series-title":"Am J Vet Res","first-page":"11","article-title":"Experimentally induced arthritis of the equine carpus: clinical determinations","volume":"40","author":"McIlwraith","year":"1979"},{"key":"ref28","series-title":"J Daily Sci","doi-asserted-by":"publisher","first-page":"3731","DOI":"10.3168\/jds.2009-2058","article-title":"Analgesic efficacy of sodium salicylate in an amphotericin B-induced bovine synovitis-arthritis model","volume":"92","author":"Kotschwar","year":"2009"},{"key":"ref29","series-title":"Vet Clin North Am Food Anim Pract","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S0749-0720(15)30053-0","article-title":"Lameness examination in cattle","volume":"17","author":"Desrochers","year":"2001"},{"key":"ref30","series-title":"Comput Electron Agric","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.compag.2009.03.002","article-title":"Evaluation of three-dimensional accelerometers to monitor and classify behavior in cattle","volume":"67","author":"Robert","year":"2009"},{"key":"ref31","series-title":"J Vet Pharmacol Ther","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1111\/j.1365-2885.2007.00869.x","article-title":"Attenuation of acute plasma Cortisol response in calves following intravenous sodium salicylate administration prior to castration","volume":"30","author":"Coetzee","year":"2007"},{"key":"ref32","series-title":"Dutch J Vet Med","first-page":"1237","article-title":"Determination of weight bearing by the cow's foot","volume":"5","author":"Toussaint-Raven","year":"1973"},{"key":"ref33","series-title":"Zentralbl Veterinarmed A","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1111\/j.1439-0442.1987.tb00283.x","article-title":"Distribution of load between the lateral and medial hoof of the bovine hind limb","volume":"34","author":"Ossent","year":"1987"},{"key":"ref34","series-title":"J Dairy Sci","doi-asserted-by":"publisher","first-page":"1512","DOI":"10.3168\/jds.2008-1578","article-title":"Effects of local anesthetic and a nonsteroidal anti-inflammatory drug on pain responses of dairy calves to hot iron dehorning","volume":"92","author":"Stewart","year":"2009"},{"key":"ref35","series-title":"J Dairy Sci","doi-asserted-by":"publisher","first-page":"720","DOI":"10.3168\/jds.S0022-0302(99)75289-6","article-title":"Fear of people by cows and effects on milk yield, behavior, and heart rate at milking","volume":"82","author":"Rushen","year":"1999"},{"key":"ref36","series-title":"Vet Clin North Am Food Anim Pract","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.cvfa.2004.12.008","article-title":"Pain management in ruminants","volume":"21","author":"Anderson","year":"2005"},{"key":"ref37","series-title":"J Vet Pharmacol Ther","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1111\/j.1365-2885.1995.tb00589.x","article-title":"Pharmacokinetics of flunixin and its effect on prostaglandin F2 alpha metabolite concentrations after oral and intravenous administration in heifers","volume":"18","author":"Odensvik","year":"1995"},{"key":"ref38","series-title":"J Dairy Sci","doi-asserted-by":"publisher","first-page":"3010","DOI":"10.3168\/jds.2007-0968","article-title":"Analgesics improve the gait of lame dairy cattle","volume":"91","author":"Flower","year":"2008"},{"key":"ref39","series-title":"Br Vet J","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/0007-1935(89)90005-5","article-title":"Changes in limb loading with lameness for a number of Friesian cattle","volume":"145","author":"Scott","year":"1989"},{"key":"ref40","series-title":"Vet Rec","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1136\/vr.163.20.601","article-title":"Locomotion scoring of cattle using a lameness-speed index on different types of track","volume":"163","author":"Berry","year":"2008"},{"key":"ref41","series-title":"Lab Anim Sci","first-page":"571","article-title":"Animal well-being II. Stress and distress","volume":"47","author":"Clark","year":"1997"},{"key":"ref42","series-title":"Vet J","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.tvjl.2004.02.005","article-title":"Dehorning and disbudding distress and its alleviation in calves","volume":"169","author":"Stafford","year":"2005"},{"key":"ref43","series-title":"J Am Vet Med Assoc","first-page":"1212","article-title":"Physiologic basis and consequences of distress in animals","volume":"191","author":"Breazile","year":"1987"},{"key":"ref44","series-title":"Am J Vet Res","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.2460\/ajvr.69.8.1005","article-title":"Evaluation of two-dimensional accelerometers to monitor behavior of beef calves after castration","volume":"69","author":"White","year":"2008"}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/72\/11\/ajvr.72.11.1431.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/72\/11\/ajvr.72.11.1431.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/72\/11\/ajvr.72.11.1431.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T12:26:36Z","timestamp":1741350396000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/72\/11\/ajvr.72.11.1431.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":44,"journal-issue":{"issue":"11"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.72.11.1431","relation":{},"ISSN":["0002-9645"],"issn-type":[{"value":"0002-9645","type":"print"}],"subject":[],"published":{"date-parts":[[2011,11]]}}}