{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:23:49Z","timestamp":1788942229356,"version":"build-2803163510"},"reference-count":47,"publisher":"American Veterinary Medical Association (AVMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To determine the effects of once-daily oral administration of N-acetyl-d-glucosamine (NAG) on plasma and urine glycosaminoglycan (GAG) concentrations in cats with idiopathic cystitis (IC).<\/jats:p>\n<jats:p><jats:bold>Animals<\/jats:bold>\u201419 cats with IC and 10 clinically normal cats.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014Cats with IC were randomly assigned to receive 250 mg of NAG in capsule form orally once daily for 28 days (n = 12) or a placebo (capsule containing cellulose) orally once daily for the same period (7). In cats with IC, plasma and urine GAG concentrations and urine creatinine concentration were measured on days 0 (immediately before first dose), 7, 14, 21, 28, and 56. For purposes of comparison, those variables were measured in 10 clinically normal cats on day 0.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014Mean \u00b1 SEM urine GAG-to-creatinine concentration ratios (day 0 data) for cats with IC and clinically normal cats differed significantly (3.11 \u00b1 0.62 \u03bcg\/mL and 14.23 \u00b1 3.47 \u03bcg\/mL, respectively). For cats with IC, mean plasma GAG concentration in NAG-treated cats (39.96 \u00b1 5.34 \u03bcg\/mL) was higher than that in placebo-treated cats (24.20 \u00b1 3.35 \u03bcg\/mL) on day 21. In the NAG-treated cats, plasma GAG concentration on days 21 (39.96 \u00b1 5.34 \u03bcg\/mL) and 28 (39.91 \u00b1 6.74 \u03bcg\/mL) differed significantly from the day 0 concentration (27.46 \u00b1 3.90\u03bcg\/mL).<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Cats with IC have lower urinary GAG-to-creatinine concentration ratios than did clinically normal cats. Administration of NAG (250 mg, PO, q 24 h) significantly increased plasma GAG concentrations in cats with IC after 21 days of treatment.<\/jats:p>","DOI":"10.2460\/ajvr.72.6.843","type":"journal-article","created":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T07:51:55Z","timestamp":1306914715000},"page":"843-850","source":"Crossref","is-referenced-by-count":20,"title":["Effects of oral administration of N-acetyl-d-glucosamine on plasma and urine concentrations of glycosaminoglycans in cats with idiopathic 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disease","volume":"35","author":"Hostutler","year":"2005"},{"key":"ref3","series-title":"J Am Vet Med Assoc","first-page":"46","article-title":"Clinical evaluation of cats with nonobstructive urinary tract diseases","volume":"210","author":"Buffington","year":"1997"},{"key":"ref4","series-title":"J Am Vet Med Assoc","first-page":"211","article-title":"Clinical evaluation of cats with lower urinary tract disease","volume":"199","author":"Kruger","year":"1991"},{"key":"ref5","series-title":"J Feline Med Surg","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S1098-612X(02)00129-8","article-title":"Feline lower urinary tract disease","volume":"5","author":"Gunn-Moore","year":"2003"},{"key":"ref6","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/S0195-5616(96)50212-3","article-title":"Interstitial cystitis in cats","volume":"26","author":"Buffington","year":"1996"},{"key":"ref7","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1016\/j.cvsm.2004.03.002","article-title":"Feline idiopathic cystitis: current understanding of pathophysiology and management","volume":"34","author":"Westropp","year":"2004"},{"key":"ref8","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.cvsm.2007.01.009","article-title":"Evidence-based management of feline lower urinary tract disease","volume":"37","author":"Dru Forrester","year":"2007"},{"key":"ref9","series-title":"J Pharmacol Exp Ther","first-page":"818","article-title":"Effect of interstitial cystitis on drug absorption from urinary bladder","volume":"271","author":"Gao","year":"1994"},{"key":"ref10","series-title":"Physiol Genomics","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1152\/physiolgenomics.00055.2003","article-title":"Changes in human bladder epithelial cell gene expression associated with interstitial cystitis or antiproliferative factor treatment","volume":"14","author":"Keay","year":"2003"},{"key":"ref11","series-title":"J Feline Med Surg","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/j.jfms.2006.02.002","article-title":"Clinical evaluation of multimodal environmental modification (MEMO) in the management of cats with idiopathic cystitis","volume":"8","author":"Buffington","year":"2006"},{"key":"ref12","series-title":"Science","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1126\/science.6154316","article-title":"Bladder-surface glycosaminoglycans: an efficient mechanism of environmental adaptation","volume":"208","author":"Parsons","year":"1980"},{"key":"ref13","series-title":"J Urol","doi-asserted-by":"publisher","first-page":"1801","DOI":"10.1016\/S0022-5347(01)66201-3","article-title":"Decreased urine glycosaminoglycan excretion in cats with interstitial cystitis","volume":"155","author":"Buffington","year":"1996"},{"key":"ref14","series-title":"Biochim Biophys Acta","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.bbagen.2004.02.002","article-title":"Changes in cat urinary glycosaminoglycans with age and in feline urologic syndrome","volume":"1672","author":"Pereira","year":"2004"},{"key":"ref15","series-title":"Int Urol Nephrol","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1023\/A:1007198708404","article-title":"Glycosaminoglycans excretion in interstitial cystitis","volume":"31","author":"Akcay","year":"1999"},{"key":"ref16","series-title":"J Urol","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1016\/S0022-5347(17)50747-8","article-title":"A methodology for the characterization of urinary glycosaminoglycans","volume":"131","author":"Thorne","year":"1984"},{"key":"ref17","series-title":"Br J Urol","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1046\/j.1464-410X.1997.03384.x","article-title":"A placebo-controlled study of intravesical pentosanpolysulphate for the treatment of interstitial cystitis","volume":"79","author":"Bade","year":"1997"},{"key":"ref18","series-title":"J Urol","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.juro.2007.08.170","article-title":"Safety and efficacy of the use of intravesical and oral pentosan polysulfate sodium for interstitial cystitis: a randomized double-blind clinical trial","volume":"179","author":"Davis","year":"2008"},{"issue":"suppl 4","key":"ref19","series-title":"Eur Urol","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/S1569-9056(03)00034-4","article-title":"The interstitial cystitis syndrome: intravesical and oral treatment","volume":"2","author":"Kurth","year":"2003"},{"key":"ref20","series-title":"Anal Chem","first-page":"464","article-title":"Enzymatic determination of urea","volume":"49","author":"Patton","year":"1977"},{"key":"ref21","series-title":"Clin Chem","doi-asserted-by":"publisher","first-page":"1419","DOI":"10.1093\/clinchem\/18.11.1419","article-title":"Simple, rapid, kinetic method for serum creatinine measurement","volume":"18","author":"Lustgarten","year":"1972"},{"key":"ref22","series-title":"Am J Clin Pathol","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1093\/ajcp\/28.1.56","article-title":"A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases","volume":"28","author":"Reitman","year":"1957"},{"key":"ref23","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/S0021-9258(18)43072-4","article-title":"A method for the rapid determination of alkaline phosphatase with five cubic millimetres of serum","volume":"164","author":"Bessey","year":"1946"},{"key":"ref24","series-title":"Anal Biochem","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976"},{"key":"ref25","series-title":"Clin Chem","doi-asserted-by":"publisher","first-page":"1472","DOI":"10.1093\/clinchem\/35.7.1472","article-title":"Dimethylmethylene blue-based spectrophotometry of glycosaminoglycans in untreated urine: a rapid screening procedure for mucopolysaccharidoses","volume":"35","author":"de Jong","year":"1989"},{"key":"ref26","series-title":"Biochim Biophys Acta","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/0304-4165(86)90306-5","article-title":"Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue","volume":"883","author":"Farndale","year":"1986"},{"key":"ref27","series-title":"Clin Chem","doi-asserted-by":"publisher","first-page":"2073","DOI":"10.1093\/clinchem\/32.11.2073","article-title":"Simple spectrophotometric quantification of urinary excretion of glycosaminoglycan sulfates","volume":"32","author":"Panin","year":"1986"},{"key":"ref28","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"1429","DOI":"10.2460\/javma.2001.218.1429","article-title":"Epidemiologic study of risk factors for lower urinary tract diseases in cats","volume":"218","author":"Lekcharoensuk","year":"2001"},{"key":"ref29","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1016\/S0195-5616(96)50086-0","article-title":"Management of nonobstructive idiopathic feline lower urinary tract disease","volume":"26","author":"Kruger","year":"1996"},{"key":"ref30","series-title":"J Urol","doi-asserted-by":"publisher","first-page":"1242","DOI":"10.1097\/01.ju.0000137953.49304.6c","article-title":"Comorbidity of interstitial cystitis with other unexplained clinical conditions","volume":"172","author":"Buffington","year":"2004"},{"key":"ref31","series-title":"J Urol","doi-asserted-by":"publisher","first-page":"2494","DOI":"10.1097\/01.ju.0000095566.63870.66","article-title":"Small adrenal glands in cats with feline interstitial cystitis","volume":"170","author":"Westropp","year":"2003"},{"key":"ref32","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1016\/j.cvsm.2006.08.006","article-title":"Nutrition and lower urinary tract disease in cats","volume":"36","author":"Bartges","year":"2006"},{"key":"ref33","series-title":"J Feline Med Surg","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.jfms.2003.09.007","article-title":"Oral glucosamine and the management of feline idiopathic cystitis","volume":"6","author":"Gunn-Moore","year":"2004"},{"key":"ref34","series-title":"J Am Vet Med Assoc","first-page":"361","article-title":"Clinical evaluation of commercially available urinary acidification diets in the management of idiopathic cystitis in cats","volume":"214","author":"Markwell","year":"1999"},{"key":"ref35","series-title":"Food Chem Toxicol","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/j.fct.2003.12.005","article-title":"Subchronic toxicity study of dietary N-acetylglucosamine in F344 rats","volume":"42","author":"Lee","year":"2004"},{"key":"ref36","series-title":"Microbiol Immunol","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1111\/j.1348-0421.1986.tb03004.x","article-title":"Effect of N-acetylchito-oligosaccharides on activation of phagocytes","volume":"30","author":"Suzuki","year":"1986"},{"key":"ref37","series-title":"Vet Clin North Am Small Anim Pract","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/S0195-5616(96)50200-7","article-title":"Feline lower urinary tract disorders. Definition of terms and concepts","volume":"26","author":"Osborne","year":"1996"},{"key":"ref38","series-title":"Carb Polym","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/S0144-8617(98)00019-8","article-title":"Effect of N-acetyl-d-glucosamine and D-glucosamine oligomers on canine polymorphonuclear cells in vitro","volume":"36","author":"Usami","year":"1998"},{"key":"ref39","series-title":"J Feline Med Surg","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1053\/jfms.2001.0118","article-title":"An investigation into the effects of storage on the diagnosis of crystalluria in cats","volume":"3","author":"Sturgess","year":"2001"},{"key":"ref40","series-title":"J Am Vet Med Assoc","doi-asserted-by":"publisher","first-page":"176","DOI":"10.2460\/javma.2003.222.176","article-title":"Effects of storage time and temperature on pH, specific gravity, and crystal formation in urine samples from dogs and cats","volume":"222","author":"Albasan","year":"2003"},{"key":"ref41","series-title":"J Urol","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/S0022-5347(17)40061-9","article-title":"Decreased urinary uronic acid levels in individuals with interstitial cystitis","volume":"143","author":"Parsons","year":"1990"},{"key":"ref42","series-title":"J Urol","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S0022-5347(17)35990-6","article-title":"Urinary glycosaminoglycan excretion as a laboratory marker in the diagnosis of interstitial cystitis","volume":"149","author":"Hurst","year":"1993"},{"key":"ref43","series-title":"Clin Ther","doi-asserted-by":"publisher","first-page":"1184","DOI":"10.1016\/S0149-2918(96)80073-7","article-title":"Pilot study of oral polymeric N-acetyl-d-glucosamine as a potential treatment for patients with osteoarthritis","volume":"18","author":"Talent","year":"1996"},{"key":"ref44","series-title":"J Biol Chem","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/S0021-9258(18)93919-0","article-title":"Metabolism of D-glucosamine and N-acetyl-d-glucosamine in the intact rat","volume":"237","author":"Kohn","year":"1962"},{"key":"ref45","series-title":"Biochem J","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1042\/bj0870354","article-title":"Mammalian glycosidases. 4. The intracellular localization of beta-galactosidase, alpha-mannosidase, beta-N-acetylglucosaminidase and alpha-L-fucosidase in mammalian tissues","volume":"87","author":"Conchie","year":"1963"},{"key":"ref46","series-title":"Adv Exp Med Biol","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/978-1-4615-1267-7_7","article-title":"Galectins and other endogenous carbohydrate-binding proteins of animal bladder","volume":"491","author":"Bhavanandan","year":"2001"},{"key":"ref47","series-title":"Int Cong Ser","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/S0531-5131(01)00433-2","article-title":"Use of glycosaminoglycans in the treatment of interstitial cystitis: a strategy to improve efficacy","volume":"1223","author":"Bhavanandan","year":"2001"}],"container-title":["American Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/72\/6\/ajvr.72.6.843.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/72\/6\/ajvr.72.6.843.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/avmajournals.avma.org\/downloadpdf\/journals\/ajvr\/72\/6\/ajvr.72.6.843.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T12:28:29Z","timestamp":1741350509000},"score":1,"resource":{"primary":{"URL":"https:\/\/avmajournals.avma.org\/view\/journals\/ajvr\/72\/6\/ajvr.72.6.843.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6]]},"references-count":47,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.2460\/ajvr.72.6.843","relation":{},"ISSN":["0002-9645"],"issn-type":[{"value":"0002-9645","type":"print"}],"subject":[],"published":{"date-parts":[[2011,6]]}}}