{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:03:48Z","timestamp":1790168628721,"version":"4.1.0"},"reference-count":31,"publisher":"American Veterinary Medical Association (AVMA)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ajvr"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:bold>Objective<\/jats:bold>\u2014To isolate and characterize bacteriophages with strong in vitro lytic activity against various pathogenic <jats:italic>Pseudomonas aeruginosa<\/jats:italic> strains isolated from dogs with ocular infections.<\/jats:p>\n<jats:p><jats:bold>Sample<\/jats:bold>\u201426 genetically distinct <jats:italic>P aeruginosa<\/jats:italic> isolates.<\/jats:p>\n<jats:p><jats:bold>Procedures<\/jats:bold>\u2014<jats:italic>P aeruginosa<\/jats:italic> strains were derived from dogs with naturally acquired ulcerative keratitis. From a large-scale screening for bacteriophages with potential therapeutic benefit against canine ocular infections, 2 bacteriophages (P2S2 and P5U5) were selected; host ranges were determined, and phage nucleic acid type and genetic profile were identified via enzymatic digestion. Electron microscopy was used to characterize bacteriophage ultrastructure. Bacteriophage temperature and pH stabilities were assessed by use of double-layer agar overlay titration. A cocultivation assay was used to evaluate the effect of the bacteriophages on bacterial host growth.<\/jats:p>\n<jats:p><jats:bold>Results<\/jats:bold>\u2014P5U5 was active against all <jats:italic>P aeruginosa<\/jats:italic> isolates, whereas P2S2 formed lytic plaques on plates of 21 (80.8%) isolates. For each bacteriophage, the genomic nucleic acid was DNA; each was genetically distinct. Ultrastructurally, P2S2 and P5U5 appeared likely to belong to the <jats:italic>Podoviridae<\/jats:italic> and <jats:italic>Siphoviridae<\/jats:italic> families, respectively. The bacteriophages were stable within a pH range of 4 to 12; however, titers of both bacteriophages decreased following heating for 10 to 50 minutes at 45\u00b0 or 60\u00b0C. Growth of each <jats:italic>P aeruginosa<\/jats:italic> isolate was significantly inhibited in coculture with P2S2 or P5U5; the dose response was related to the plaque-forming unit-to-CFU ratios.<\/jats:p>\n<jats:p><jats:bold>Conclusions and Clinical Relevance<\/jats:bold>\u2014Bacteriophages P2S2 and P5U5 appear to be good candidates for phage treatment of infection caused by pathogenic <jats:italic>P aeruginosa<\/jats:italic> in dogs.<\/jats:p>","DOI":"10.2460\/ajvr.72.8.1079","type":"journal-article","created":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T07:09:30Z","timestamp":1312182570000},"page":"1079-1086","source":"Crossref","is-referenced-by-count":19,"title":["Isolation and characterization of two bacteriophages with strong in vitro antimicrobial activity against Pseudomonas aeruginosa isolated from dogs with ocular infections"],"prefix":"10.2460","volume":"72","author":[{"given":"Thiago M. 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